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• W2003446885 startingPage "343" @default.
• W2003446885 abstract "The fluorescent derivative of the actin-binding toxin phallacidin, 7-nitrobenz-2-oxa-1,3 diazole phallacidin, has been used to cytologically demonstrate the presence of actin in lens epithelium, corneal endothelium, and retinal pigment epithelium. In these noninjured tissues, no stress fibers are observed and fluorescence is confined mainly to an area at or near the cell membrane, although some diffuse cytoplasmic staining can also be seen. However, following injury to either the lens epithelium or corneal endothelium of rats and frogs, stress fibers are detected, but only in those cells that migrate into the wound area. Cells on the periphery of each tissue do not partake in would repair and thus maintain their normal appearance. After the tissue has regenerated, stress fibers disappear, and those cells involved in the injury response return to their normal morphology. When rabbit corneal endothelium is placed in tissue culture, stress fibers are observed as the cells migrate away from the initial explant. Upon reaching confluency, these cells spread out and each is surrounded by thick actin-containing bands. Furthermore, they exhibit some stress cables within their cytoplasm. This is in contrast to their appearance in vivo where stress fibers are absent and fluorescence is limited to a region near the cell membrane." @default.
• W2003446885 created "2016-06-24" @default.
• W2003446885 creator A5012182252 @default.
• W2003446885 creator A5023378323 @default.
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• W2003446885 date "1982-01-01" @default.
• W2003446885 modified "2023-09-30" @default.
• W2003446885 title "In situ demonstration of actin in normal and injured ocular tissues using 7-nitrobenz-2-oxa-1,3-diazole phallacidin" @default.
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• W2003446885 doi "https://doi.org/10.1002/cm.970020404" @default.
• W2003446885 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6983909" @default.
• W2003446885 hasPublicationYear "1982" @default.
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