Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003448134> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2003448134 endingPage "284" @default.
- W2003448134 startingPage "273" @default.
- W2003448134 abstract "The antigenic profiles of three REV prototype strains, CSV, SNV and REV-T and eight Israeli isolates were analysed by SDS-PAGE and immunoblotting with convalescent chicken serum, three mAbs, 11A25, 11C237 and 11C100, a rabbit antiserum to REV-T whole virus (Cui et al., 1986) and a rabbit antiserum to REV-A p30 gag protein (Tsai et al., 1985). Under both reducing (+DTT) and non-reducing conditions of SDS-PAGE, a major immunodominant 75–100 kDa band was shared by all strains examined. In contrast to the chicken serum that recognized both continuous and discontinuous epitopes on the 75–100 kDa band of all the isolates, the mAbs and the two rabbit sera behaved otherwise. Only the DTT-resistant epitopes on the 75–100 kDa band of REV-T were recognized by the rabbit antisera and the mAb 11C237, and only the DTT-labile epitopes of REV-T 75–100 kDa antigen were detected by mAb 11C100. The two mAbs 11A25 and 11C237 detected discontinuous epitopes of all the strains except SNV, while the rabbit antisera recognized the discontinuous epitopes on the 75–100 kDa band of all the 11 strains. The rabbit antisera and mAb 11C237 detected additional lower molecular weight proteins and the mAb 11C237 also detected three proteins of high molecular weight under non-reducing conditions only. The p30 antiserum detected the low molecular weight proteins demonstrating their gag gene-encoded identity. From these results we conclude that the major immunogen of REV is the 75–100 kDa protein that contains both continuous and discontinous epitopes. With this panel of antibodies the eight new isolates appeared to belong antigenically to REV subtype 3 (Chen et al., 1987)." @default.
- W2003448134 created "2016-06-24" @default.
- W2003448134 creator A5018289357 @default.
- W2003448134 creator A5049445988 @default.
- W2003448134 creator A5051908855 @default.
- W2003448134 creator A5053333537 @default.
- W2003448134 date "1996-04-01" @default.
- W2003448134 modified "2023-09-25" @default.
- W2003448134 title "The immunodominant proteins of reticuloendotheliosis virus" @default.
- W2003448134 cites W1523613058 @default.
- W2003448134 cites W1550023838 @default.
- W2003448134 cites W1610462275 @default.
- W2003448134 cites W1970503096 @default.
- W2003448134 cites W1998249443 @default.
- W2003448134 cites W2016760730 @default.
- W2003448134 cites W2023984047 @default.
- W2003448134 cites W2030511750 @default.
- W2003448134 cites W2041875681 @default.
- W2003448134 cites W2062188084 @default.
- W2003448134 cites W2091165044 @default.
- W2003448134 cites W2093815956 @default.
- W2003448134 cites W2100837269 @default.
- W2003448134 cites W2333539307 @default.
- W2003448134 cites W2400597826 @default.
- W2003448134 cites W4231137680 @default.
- W2003448134 doi "https://doi.org/10.1016/0378-1135(95)00183-2" @default.
- W2003448134 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8734645" @default.
- W2003448134 hasPublicationYear "1996" @default.
- W2003448134 type Work @default.
- W2003448134 sameAs 2003448134 @default.
- W2003448134 citedByCount "16" @default.
- W2003448134 countsByYear W20034481342012 @default.
- W2003448134 countsByYear W20034481342013 @default.
- W2003448134 countsByYear W20034481342020 @default.
- W2003448134 crossrefType "journal-article" @default.
- W2003448134 hasAuthorship W2003448134A5018289357 @default.
- W2003448134 hasAuthorship W2003448134A5049445988 @default.
- W2003448134 hasAuthorship W2003448134A5051908855 @default.
- W2003448134 hasAuthorship W2003448134A5053333537 @default.
- W2003448134 hasConcept C147483822 @default.
- W2003448134 hasConcept C153911025 @default.
- W2003448134 hasConcept C159047783 @default.
- W2003448134 hasConcept C159654299 @default.
- W2003448134 hasConcept C195616568 @default.
- W2003448134 hasConcept C203014093 @default.
- W2003448134 hasConcept C2522874641 @default.
- W2003448134 hasConcept C2776525413 @default.
- W2003448134 hasConcept C2779909307 @default.
- W2003448134 hasConcept C542903549 @default.
- W2003448134 hasConcept C86803240 @default.
- W2003448134 hasConcept C91523082 @default.
- W2003448134 hasConceptScore W2003448134C147483822 @default.
- W2003448134 hasConceptScore W2003448134C153911025 @default.
- W2003448134 hasConceptScore W2003448134C159047783 @default.
- W2003448134 hasConceptScore W2003448134C159654299 @default.
- W2003448134 hasConceptScore W2003448134C195616568 @default.
- W2003448134 hasConceptScore W2003448134C203014093 @default.
- W2003448134 hasConceptScore W2003448134C2522874641 @default.
- W2003448134 hasConceptScore W2003448134C2776525413 @default.
- W2003448134 hasConceptScore W2003448134C2779909307 @default.
- W2003448134 hasConceptScore W2003448134C542903549 @default.
- W2003448134 hasConceptScore W2003448134C86803240 @default.
- W2003448134 hasConceptScore W2003448134C91523082 @default.
- W2003448134 hasIssue "3-4" @default.
- W2003448134 hasLocation W20034481341 @default.
- W2003448134 hasLocation W20034481342 @default.
- W2003448134 hasOpenAccess W2003448134 @default.
- W2003448134 hasPrimaryLocation W20034481341 @default.
- W2003448134 hasRelatedWork W1604248381 @default.
- W2003448134 hasRelatedWork W1679554208 @default.
- W2003448134 hasRelatedWork W1973692620 @default.
- W2003448134 hasRelatedWork W1991473484 @default.
- W2003448134 hasRelatedWork W2060284918 @default.
- W2003448134 hasRelatedWork W2070226647 @default.
- W2003448134 hasRelatedWork W2073771134 @default.
- W2003448134 hasRelatedWork W2146940419 @default.
- W2003448134 hasRelatedWork W216175594 @default.
- W2003448134 hasRelatedWork W2373117620 @default.
- W2003448134 hasVolume "49" @default.
- W2003448134 isParatext "false" @default.
- W2003448134 isRetracted "false" @default.
- W2003448134 magId "2003448134" @default.
- W2003448134 workType "article" @default.