Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003459185> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2003459185 abstract "The pathogenesis of many protein disorders is characterized by the conversion of a constitutive soluble protein into a less soluble beta-sheet rich conformation. In the case of Alzheimer's disease (AD), assembly of amyloid beta (Abeta) protein into neurotoxic oligomers is reported to be a likely cause of disease. Diagnostic tools that can distinguish between different conformations of the Abeta protein biomarker would be a unique and valuable contribution to the field. We have previously reported the use of small conformationally dynamic peptides (Pronucleon™ peptides), that enable the detection of beta sheet amyloid proteins both in prion disease and in AD. These fluorescently-labeled peptides undergo a sequence-specific conformational rearrangement in the presence of the beta-sheet structure of Abeta aggregates. The rearrangement results in a change in the fluorescence profile that can be monitored using standard laboratory instrumentation. Toward the overall goal of creating an in vitro diagnostic test that directly measures peptide molecular structures implicated in AD, we tested a system of specifically defined amyloid aggregates and spiked these into clinical matrices including cerebrospinal fluid (CSF). We addressed issues of assay design, dose-response, and target availability in this biological matrix. We have created controlled populations of soluble Abeta aggregates prepared from purchased recombinant Abeta. Assay studies were performed using Adlyfe's MPD (“misfolded” protein detection) assay which combines sample and Pronucleon peptide in a 96-well plate and follows the fluorescence transition associated with conformational changes of the Pronucleon peptide in the presence of Abeta aggregates. In previous studies, we demonstrated reactivity with insoluble Abeta “fiber” aggregate. In this work, we extend our assay to soluble Abeta aggregates. We have evaluated variations in the sequence of the Pronucleon peptides, considering structural regions of Abeta folding and aggregation. We demonstrate detection of synthetic aggregate forms and present data on the translation of assay conditions to clinical samples representing cohorts from subjects with AD or mild cognitive impairment versus age-matched controls. The Pronucleon peptide technology demonstrates specific recognition of beta-sheet rich Abeta aggregates and the potential to create a diagnostic test for the peptide biomarkers of AD." @default.
- W2003459185 created "2016-06-24" @default.
- W2003459185 creator A5010188932 @default.
- W2003459185 creator A5022682916 @default.
- W2003459185 creator A5033668962 @default.
- W2003459185 creator A5039535686 @default.
- W2003459185 creator A5040856063 @default.
- W2003459185 creator A5049888868 @default.
- W2003459185 creator A5050980294 @default.
- W2003459185 creator A5060408204 @default.
- W2003459185 creator A5082386681 @default.
- W2003459185 date "2008-07-01" @default.
- W2003459185 modified "2023-09-27" @default.
- W2003459185 title "P3-091: Specific conformational detection of beta-sheet rich amyloid beta proteins using conformationally dynamic Pronucleon™ peptides" @default.
- W2003459185 doi "https://doi.org/10.1016/j.jalz.2008.05.1655" @default.
- W2003459185 hasPublicationYear "2008" @default.
- W2003459185 type Work @default.
- W2003459185 sameAs 2003459185 @default.
- W2003459185 citedByCount "0" @default.
- W2003459185 crossrefType "journal-article" @default.
- W2003459185 hasAuthorship W2003459185A5010188932 @default.
- W2003459185 hasAuthorship W2003459185A5022682916 @default.
- W2003459185 hasAuthorship W2003459185A5033668962 @default.
- W2003459185 hasAuthorship W2003459185A5039535686 @default.
- W2003459185 hasAuthorship W2003459185A5040856063 @default.
- W2003459185 hasAuthorship W2003459185A5049888868 @default.
- W2003459185 hasAuthorship W2003459185A5050980294 @default.
- W2003459185 hasAuthorship W2003459185A5060408204 @default.
- W2003459185 hasAuthorship W2003459185A5082386681 @default.
- W2003459185 hasConcept C104317684 @default.
- W2003459185 hasConcept C12554922 @default.
- W2003459185 hasConcept C136238340 @default.
- W2003459185 hasConcept C142724271 @default.
- W2003459185 hasConcept C159547669 @default.
- W2003459185 hasConcept C179104552 @default.
- W2003459185 hasConcept C185592680 @default.
- W2003459185 hasConcept C202751555 @default.
- W2003459185 hasConcept C2776156579 @default.
- W2003459185 hasConcept C2777339483 @default.
- W2003459185 hasConcept C2777633098 @default.
- W2003459185 hasConcept C2778670691 @default.
- W2003459185 hasConcept C2779134260 @default.
- W2003459185 hasConcept C2779281246 @default.
- W2003459185 hasConcept C40767141 @default.
- W2003459185 hasConcept C502032728 @default.
- W2003459185 hasConcept C55493867 @default.
- W2003459185 hasConcept C71924100 @default.
- W2003459185 hasConcept C86803240 @default.
- W2003459185 hasConceptScore W2003459185C104317684 @default.
- W2003459185 hasConceptScore W2003459185C12554922 @default.
- W2003459185 hasConceptScore W2003459185C136238340 @default.
- W2003459185 hasConceptScore W2003459185C142724271 @default.
- W2003459185 hasConceptScore W2003459185C159547669 @default.
- W2003459185 hasConceptScore W2003459185C179104552 @default.
- W2003459185 hasConceptScore W2003459185C185592680 @default.
- W2003459185 hasConceptScore W2003459185C202751555 @default.
- W2003459185 hasConceptScore W2003459185C2776156579 @default.
- W2003459185 hasConceptScore W2003459185C2777339483 @default.
- W2003459185 hasConceptScore W2003459185C2777633098 @default.
- W2003459185 hasConceptScore W2003459185C2778670691 @default.
- W2003459185 hasConceptScore W2003459185C2779134260 @default.
- W2003459185 hasConceptScore W2003459185C2779281246 @default.
- W2003459185 hasConceptScore W2003459185C40767141 @default.
- W2003459185 hasConceptScore W2003459185C502032728 @default.
- W2003459185 hasConceptScore W2003459185C55493867 @default.
- W2003459185 hasConceptScore W2003459185C71924100 @default.
- W2003459185 hasConceptScore W2003459185C86803240 @default.
- W2003459185 hasIssue "4S_Part_16" @default.
- W2003459185 hasLocation W20034591851 @default.
- W2003459185 hasOpenAccess W2003459185 @default.
- W2003459185 hasPrimaryLocation W20034591851 @default.
- W2003459185 hasRelatedWork W1969967781 @default.
- W2003459185 hasRelatedWork W1972944045 @default.
- W2003459185 hasRelatedWork W1998227221 @default.
- W2003459185 hasRelatedWork W2003459185 @default.
- W2003459185 hasRelatedWork W2027773183 @default.
- W2003459185 hasRelatedWork W2060720180 @default.
- W2003459185 hasRelatedWork W2136860964 @default.
- W2003459185 hasRelatedWork W2529551069 @default.
- W2003459185 hasRelatedWork W2753608750 @default.
- W2003459185 hasRelatedWork W3136352688 @default.
- W2003459185 hasVolume "4" @default.
- W2003459185 isParatext "false" @default.
- W2003459185 isRetracted "false" @default.
- W2003459185 magId "2003459185" @default.
- W2003459185 workType "article" @default.