Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003489394> ?p ?o ?g. }
- W2003489394 endingPage "382" @default.
- W2003489394 startingPage "377" @default.
- W2003489394 abstract "ATP and other purine nucleotides are important biomarkers for ischemia and may have considerable potential as targets for management of ischemic heart disease and stroke. The main objective of the study is to develop a rapid HPLC assay, which has adequate sensitivity and specificity for measuring concentrations of ATP, ADP, AMP, GTP, GDP and GMP in erythrocytes (RBC). The assays used ion-pair chromatography coupled with ultraviolet detection at 254 nm to separate and detect the purine nucleotides. Using 50-100 microL of RBC lysate as blank biologic matrix, the assay was linear from 100 to 2000 microg/mL for ATP and ADP, and 20-400 microg/mL for AMP, GTP, and GDP with coefficients of determination (r(2)) >0.99. GDP and GMP were not measurable in the study because of low concentrations and interference from endogenous materials, respectively. The intra-assay and inter-assay variations over a period of 1 year were less than 10% and 20%, respectively for most of the nucleotides. The assay was successfully applied to two pilot biomarker studies to measure RBC concentrations of the purine nucleotides in rats under restraining and exercise conditions. Preliminary results showed that the RBC concentrations of ATP and GTP were higher in the spontaneously hypertensive rats (SHR) compared to the Sprague-Dawley (SD) rats, and that exercise increased RBC concentrations of ATP in rats treated with the calcium channel blocker diltiazem." @default.
- W2003489394 created "2016-06-24" @default.
- W2003489394 creator A5066559388 @default.
- W2003489394 creator A5067516344 @default.
- W2003489394 creator A5087594961 @default.
- W2003489394 date "2008-06-01" @default.
- W2003489394 modified "2023-10-16" @default.
- W2003489394 title "HPLC assay with UV detection for determination of RBC purine nucleotide concentrations and application for biomarker study in vivo" @default.
- W2003489394 cites W111823684 @default.
- W2003489394 cites W1562460830 @default.
- W2003489394 cites W1606551511 @default.
- W2003489394 cites W165512516 @default.
- W2003489394 cites W1804853740 @default.
- W2003489394 cites W1970592808 @default.
- W2003489394 cites W1982357371 @default.
- W2003489394 cites W1986626541 @default.
- W2003489394 cites W1988848089 @default.
- W2003489394 cites W1990984872 @default.
- W2003489394 cites W1992061744 @default.
- W2003489394 cites W1993358555 @default.
- W2003489394 cites W1994509727 @default.
- W2003489394 cites W1996221936 @default.
- W2003489394 cites W1996704811 @default.
- W2003489394 cites W1997328050 @default.
- W2003489394 cites W1999086038 @default.
- W2003489394 cites W2003915488 @default.
- W2003489394 cites W2006763427 @default.
- W2003489394 cites W2008456096 @default.
- W2003489394 cites W2014420443 @default.
- W2003489394 cites W2016640448 @default.
- W2003489394 cites W2020718942 @default.
- W2003489394 cites W2022742339 @default.
- W2003489394 cites W2023128306 @default.
- W2003489394 cites W2029408805 @default.
- W2003489394 cites W2040788830 @default.
- W2003489394 cites W2040908316 @default.
- W2003489394 cites W2051836134 @default.
- W2003489394 cites W2058579821 @default.
- W2003489394 cites W2061229586 @default.
- W2003489394 cites W2064499756 @default.
- W2003489394 cites W2065653539 @default.
- W2003489394 cites W2067206754 @default.
- W2003489394 cites W2072019068 @default.
- W2003489394 cites W2072330037 @default.
- W2003489394 cites W2079160255 @default.
- W2003489394 cites W2087888115 @default.
- W2003489394 cites W2088443855 @default.
- W2003489394 cites W2091248843 @default.
- W2003489394 cites W2094074829 @default.
- W2003489394 cites W2100770709 @default.
- W2003489394 cites W2116238347 @default.
- W2003489394 cites W2117926778 @default.
- W2003489394 cites W2126431774 @default.
- W2003489394 cites W2127535589 @default.
- W2003489394 cites W2141012382 @default.
- W2003489394 cites W2143498810 @default.
- W2003489394 cites W2913929550 @default.
- W2003489394 cites W3203976090 @default.
- W2003489394 cites W4230341958 @default.
- W2003489394 cites W4243733514 @default.
- W2003489394 cites W4298091326 @default.
- W2003489394 cites W68731737 @default.
- W2003489394 doi "https://doi.org/10.1016/j.jpba.2008.01.020" @default.
- W2003489394 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18295998" @default.
- W2003489394 hasPublicationYear "2008" @default.
- W2003489394 type Work @default.
- W2003489394 sameAs 2003489394 @default.
- W2003489394 citedByCount "39" @default.
- W2003489394 countsByYear W20034893942012 @default.
- W2003489394 countsByYear W20034893942013 @default.
- W2003489394 countsByYear W20034893942014 @default.
- W2003489394 countsByYear W20034893942015 @default.
- W2003489394 countsByYear W20034893942016 @default.
- W2003489394 countsByYear W20034893942017 @default.
- W2003489394 countsByYear W20034893942018 @default.
- W2003489394 countsByYear W20034893942019 @default.
- W2003489394 countsByYear W20034893942020 @default.
- W2003489394 countsByYear W20034893942021 @default.
- W2003489394 countsByYear W20034893942022 @default.
- W2003489394 crossrefType "journal-article" @default.
- W2003489394 hasAuthorship W2003489394A5066559388 @default.
- W2003489394 hasAuthorship W2003489394A5067516344 @default.
- W2003489394 hasAuthorship W2003489394A5087594961 @default.
- W2003489394 hasConcept C104317684 @default.
- W2003489394 hasConcept C118716 @default.
- W2003489394 hasConcept C150903083 @default.
- W2003489394 hasConcept C179998833 @default.
- W2003489394 hasConcept C181199279 @default.
- W2003489394 hasConcept C185592680 @default.
- W2003489394 hasConcept C207001950 @default.
- W2003489394 hasConcept C2776476023 @default.
- W2003489394 hasConcept C43617362 @default.
- W2003489394 hasConcept C512185932 @default.
- W2003489394 hasConcept C55493867 @default.
- W2003489394 hasConcept C86803240 @default.
- W2003489394 hasConceptScore W2003489394C104317684 @default.
- W2003489394 hasConceptScore W2003489394C118716 @default.
- W2003489394 hasConceptScore W2003489394C150903083 @default.