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• W2003521113 abstract "Abstract Background : The zona pellucida (ZP), an extracellular matrix which surrounds mammalian oocytes, is formed by different glycoproteins. Several studies have revealed that carbohydrate residues present in glycoproteins of ZP play a key role in the sperm‐egg recognition. However, the origin and the biochemical composition of ZP remain to be completely resolved. Methods : ZP glycoproteins from rat ovarian follicles were investigated at light and electron microscopic level by the application of lectins conjugated to peroxidase, digoxigenin, and colloidal gold in combination with enzyme and chemical treatment. A quantitative analysis was also performed. Results : ZP shows reactivity to WGA, DSA, LFA, AAA, RCA I, and MAA. SBA and PNA showed a variable reactivity ranging from negative to strongly positive. A uniform pattern of binding throughout ZP was observed with DSA, Con A, AAA, MAA, and LFA. However, labeling by RCA I and SBA was higher in the outer ZP while PNA and WGA showed a higher binding in the inner ZP. Lectin reactivity was detected in cortical granules, endoplasmic reticulum, Golgi apparatus, vesicles, and multivesicular bodies of oocytes. Conclusions : ZP contained the terminal disaccharides Galβ1,4GlcNAc, Galβ1,3GalNAc, and GalNAcβ1,3Gal and the trisaccharides Neu5Acα2, 3Galβ1,4GlcNAc, Neu5Ac‐Galβ1,3GalNAc, and Neu5Ac‐GalNAcβ1,3Gal sequences. The occurrence of Fucose residues α 1,6 linked to the inner core region of N‐linked glycoproteins of ZP was demonstratd by the use of several fucose‐specific lectins. Methylation‐saponification treatment in combination with lectin cytochemistry reveals that Gal, GalNAc, and polyllactosamine residues of rat ZP glycoproteins contain sulphated groups. The reactivity observed in ooplasmic vesicles was similar to that of ZP, thus suggesting that the oocyte is the site of synthesis of ZP glycoproteins. © 1994 Wiley‐Liss, Inc." @default.
• W2003521113 created "2016-06-24" @default.
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• W2003521113 date "1994-06-01" @default.
• W2003521113 modified "2023-09-29" @default.
• W2003521113 title "Cytochemical characterization of oligosaccharide side chains of the glycoproteins of rat zona pellucida: An ultrastructural study" @default.
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• W2003521113 doi "https://doi.org/10.1002/ar.1092390204" @default.
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