FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003549378> ?p ?o ?g. }

• W2003549378 endingPage "273" @default.
• W2003549378 startingPage "269" @default.
• W2003549378 abstract "Murine norovirus (MNV) is a viral agent newly identified in laboratory mice and a large number of genetically diverse MNV strains have been reported to date. A broadly reactive TaqMan-based real-time reverse transcription (RT)-polymerase chain reaction (PCR) assay was developed for MNVs. Novel primers and a TaqMan MGB probe were designed targeting highly conserved sequences among MNV strains, which are located in the open reading frames 1 (ORF1)–ORF2 junction region. The quantitative range of this assay was determined as 1.0 × 102–1.0 × 108 copies/PCR tube based on a 10-fold serial dilution of plasmid DNA containing the target sequences. Viral RNA in eight murine stool specimens positive by nested RT-PCR assay was measured, and the highest viral RNA load was calculated at 4.7 × 106 copies/g-stool. MNV was inoculated into RAW 264.7 cells, and the viral RNA was monitored to validate assay sensitivity. MNV-RNA in the supernatant was detected during in vitro replication, which increased substantially from 5 to 30 h post-infection (hpi) and reached more than 1.0 × 1010 copies/mL at 96 hpi. This real-time RT-PCR assay is a useful tool to detect and quantify MNV-RNA in in vivo and in vitro studies." @default.
• W2003549378 created "2016-06-24" @default.
• W2003549378 creator A5001049546 @default.
• W2003549378 creator A5019654275 @default.
• W2003549378 creator A5022274918 @default.
• W2003549378 creator A5044603246 @default.
• W2003549378 creator A5047758255 @default.
• W2003549378 creator A5050896717 @default.
• W2003549378 creator A5076980836 @default.
• W2003549378 date "2010-11-01" @default.
• W2003549378 modified "2023-10-03" @default.
• W2003549378 title "Development and application of a broadly reactive real-time reverse transcription-PCR assay for detection of murine noroviruses" @default.
• W2003549378 cites W1577622626 @default.
• W2003549378 cites W1869731232 @default.
• W2003549378 cites W1977311181 @default.
• W2003549378 cites W1989069389 @default.
• W2003549378 cites W1997298955 @default.
• W2003549378 cites W2007810618 @default.
• W2003549378 cites W2013076066 @default.
• W2003549378 cites W2029004405 @default.
• W2003549378 cites W2040574416 @default.
• W2003549378 cites W2089545895 @default.
• W2003549378 cites W2092031427 @default.
• W2003549378 cites W2093939204 @default.
• W2003549378 cites W2109014496 @default.
• W2003549378 cites W2125124823 @default.
• W2003549378 cites W2132969117 @default.
• W2003549378 cites W2139579529 @default.
• W2003549378 cites W2149867286 @default.
• W2003549378 cites W2157218195 @default.
• W2003549378 cites W2158026372 @default.
• W2003549378 cites W2161334558 @default.
• W2003549378 cites W4211086989 @default.
• W2003549378 doi "https://doi.org/10.1016/j.jviromet.2010.07.018" @default.
• W2003549378 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20670657" @default.
• W2003549378 hasPublicationYear "2010" @default.
• W2003549378 type Work @default.
• W2003549378 sameAs 2003549378 @default.
• W2003549378 citedByCount "63" @default.
• W2003549378 countsByYear W20035493782012 @default.
• W2003549378 countsByYear W20035493782013 @default.
• W2003549378 countsByYear W20035493782014 @default.
• W2003549378 countsByYear W20035493782015 @default.
• W2003549378 countsByYear W20035493782016 @default.
• W2003549378 countsByYear W20035493782017 @default.
• W2003549378 countsByYear W20035493782018 @default.
• W2003549378 countsByYear W20035493782019 @default.
• W2003549378 countsByYear W20035493782020 @default.
• W2003549378 countsByYear W20035493782021 @default.
• W2003549378 countsByYear W20035493782022 @default.
• W2003549378 countsByYear W20035493782023 @default.
• W2003549378 crossrefType "journal-article" @default.
• W2003549378 hasAuthorship W2003549378A5001049546 @default.
• W2003549378 hasAuthorship W2003549378A5019654275 @default.
• W2003549378 hasAuthorship W2003549378A5022274918 @default.
• W2003549378 hasAuthorship W2003549378A5044603246 @default.
• W2003549378 hasAuthorship W2003549378A5047758255 @default.
• W2003549378 hasAuthorship W2003549378A5050896717 @default.
• W2003549378 hasAuthorship W2003549378A5076980836 @default.
• W2003549378 hasConcept C104317684 @default.
• W2003549378 hasConcept C105580179 @default.
• W2003549378 hasConcept C142724271 @default.
• W2003549378 hasConcept C143660080 @default.
• W2003549378 hasConcept C153911025 @default.
• W2003549378 hasConcept C156719811 @default.
• W2003549378 hasConcept C159047783 @default.
• W2003549378 hasConcept C184112429 @default.
• W2003549378 hasConcept C202751555 @default.
• W2003549378 hasConcept C204787440 @default.
• W2003549378 hasConcept C2522874641 @default.
• W2003549378 hasConcept C2777747160 @default.
• W2003549378 hasConcept C2778602366 @default.
• W2003549378 hasConcept C2778750513 @default.
• W2003549378 hasConcept C4692481 @default.
• W2003549378 hasConcept C48023723 @default.
• W2003549378 hasConcept C49105822 @default.
• W2003549378 hasConcept C54355233 @default.
• W2003549378 hasConcept C67705224 @default.
• W2003549378 hasConcept C71924100 @default.
• W2003549378 hasConcept C86803240 @default.
• W2003549378 hasConceptScore W2003549378C104317684 @default.
• W2003549378 hasConceptScore W2003549378C105580179 @default.
• W2003549378 hasConceptScore W2003549378C142724271 @default.
• W2003549378 hasConceptScore W2003549378C143660080 @default.
• W2003549378 hasConceptScore W2003549378C153911025 @default.
• W2003549378 hasConceptScore W2003549378C156719811 @default.
• W2003549378 hasConceptScore W2003549378C159047783 @default.
• W2003549378 hasConceptScore W2003549378C184112429 @default.
• W2003549378 hasConceptScore W2003549378C202751555 @default.
• W2003549378 hasConceptScore W2003549378C204787440 @default.
• W2003549378 hasConceptScore W2003549378C2522874641 @default.
• W2003549378 hasConceptScore W2003549378C2777747160 @default.
• W2003549378 hasConceptScore W2003549378C2778602366 @default.
• W2003549378 hasConceptScore W2003549378C2778750513 @default.
• W2003549378 hasConceptScore W2003549378C4692481 @default.
• W2003549378 hasConceptScore W2003549378C48023723 @default.
• W2003549378 hasConceptScore W2003549378C49105822 @default.
• W2003549378 hasConceptScore W2003549378C54355233 @default.

• next