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- W2003570003 abstract "Abstract An HPLC apparatus to trace the reaction semicontinuously was devised and applied to the kinetic study of an enzyme reaction. From the thermostated solution in which the reaction proceeds continuously, an aliquot of the solution was removed and submitted to HPLC automatically and repeatedly. Thus, the change of concentrations of reactant and/or product was determined at a definite short interval. The inhibitory action of cyclic-/ P phosphodiesterase (PDE) by several benzo[c]phenenthridine alkaloids was examined. From 1/S–1/V (Lineweaver-Burk plot) sanguinarine and chelerythrine, which were both potent bacteriocidal alkaloids, were found to strongly inhibit PDE competitively. Kinetic parameters of the enzyme reaction such as Michaelis constant and inhibition constants were obtained." @default.
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- W2003570003 date "1990-02-01" @default.
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- W2003570003 title "A Semicontinuous Assay of Inhibition of Cyclic-AMP Phosphodiesterase by Benzo[c]phenanthridine Alkaloids" @default.
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- W2003570003 doi "https://doi.org/10.1080/01483919008051804" @default.
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