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- W2003618954 abstract "A solution for staining differentially the spores and vegetative cytoplasm of bacteria, yeasts, and certain fungi has been developed. The solution is a mixture of two phenyl methane dyes, malachite green and basic fuchsin; it can be used as a concentrated aqueous solution or as a dilute saline solution. It is not necessary to use heat, though heating is recommended for staining ripe spores of bacteria. Decolorization and counterstaining are not required. Bacterial and yeast spores are stained blue or greenish-blue, vegetative cytoplasm light violet or pink. Young bacterial cells stain a deep violet, older cells light violet; the solution can thus be used as a general stain. The acid-fast organism Mycobacterium berolinensis was stained greenish-blue with the granules violet. The saline solution is recommended as a differential stain with young colonies of Aspergillus and Penicillium; the terminal growing tips and young branches of hyphae are stained blue, older hyphae light violet, spores and conidiophores blue." @default.
- W2003618954 created "2016-06-24" @default.
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- W2003618954 date "1941-04-01" @default.
- W2003618954 modified "2023-10-17" @default.
- W2003618954 title "A SOLUTION FOR STAINING DIFFERENTIALLY THE SPORES AND VEGETATIVE CELLS OF MICRO-ORGANISMS" @default.
- W2003618954 doi "https://doi.org/10.1139/cjr41c-013" @default.
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