Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003619590> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2003619590 endingPage "580" @default.
- W2003619590 startingPage "573" @default.
- W2003619590 abstract "Soybean lipoxygenase-1 (EC 1.13.11.12) reaction with linoleic acid as substrate was used to study the biocatalysis in a biphasic system when the reactants have surface-active properties. The poorly water-soluble substrate was initially dissolved in an apolar solvent (octane). The hydroperoxide produced was water soluble and remained in the aqueous phase (borate buffer). The bioreactor was a modified Lewis cell with a well-defined interfacial area between the two phases. Two phenomena were studied separately: the reactant transfer between the two phases and the biocatalyzed reaction in an aqueous medium. This allowed determination of the transfer and the reaction constants. Substrate transfer was found to be affected by the progress of the reaction, because linoleic acid and the hydroperoxy acid have an influence on the interfacial tension. Inactivation of the biocatalyst at the interface was observed in the bioreactor. These results indicate that it is impossible to analyze the system behavior with the method proposed in the literature, which is based on the sequential study of the substrate transfer to the aqueous phase and its biocatalysis by lipoxygenase. The interaction between transfer phenomena and reaction kinetics was studied in the biphasic system. The kinetics were different from those obtained in the aqueous medium. Catalysis and transfer influence each other reciprocally. In this compartmentalized system, cooperativity phenomena were obtained using a nonallosteric enzyme. The evolution of the system was modeled (Runge-Kutta algorithm). The curves obtained were very close to those determined experimentally." @default.
- W2003619590 created "2016-06-24" @default.
- W2003619590 creator A5014786221 @default.
- W2003619590 creator A5036837401 @default.
- W2003619590 creator A5051829089 @default.
- W2003619590 creator A5073116217 @default.
- W2003619590 date "2000-03-26" @default.
- W2003619590 modified "2023-10-17" @default.
- W2003619590 title "Investigation of behavior of an enzyme in a biphasic system: Soybean lipoxygenase-1" @default.
- W2003619590 cites W1531198291 @default.
- W2003619590 cites W1976904749 @default.
- W2003619590 cites W1980716340 @default.
- W2003619590 cites W1983734289 @default.
- W2003619590 cites W1994383095 @default.
- W2003619590 cites W2003383965 @default.
- W2003619590 cites W2015784659 @default.
- W2003619590 cites W2028858630 @default.
- W2003619590 cites W2030295708 @default.
- W2003619590 cites W2064374001 @default.
- W2003619590 cites W2064985385 @default.
- W2003619590 cites W2083536314 @default.
- W2003619590 cites W2136931608 @default.
- W2003619590 cites W2144224053 @default.
- W2003619590 cites W2314367955 @default.
- W2003619590 cites W4211229013 @default.
- W2003619590 cites W422585729 @default.
- W2003619590 doi "https://doi.org/10.1002/(sici)1097-0290(19960905)51:5<573::aid-bit9>3.0.co;2-9" @default.
- W2003619590 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18629821" @default.
- W2003619590 hasPublicationYear "2000" @default.
- W2003619590 type Work @default.
- W2003619590 sameAs 2003619590 @default.
- W2003619590 citedByCount "15" @default.
- W2003619590 countsByYear W20036195902019 @default.
- W2003619590 countsByYear W20036195902022 @default.
- W2003619590 crossrefType "journal-article" @default.
- W2003619590 hasAuthorship W2003619590A5014786221 @default.
- W2003619590 hasAuthorship W2003619590A5036837401 @default.
- W2003619590 hasAuthorship W2003619590A5051829089 @default.
- W2003619590 hasAuthorship W2003619590A5073116217 @default.
- W2003619590 hasConcept C111368507 @default.
- W2003619590 hasConcept C127313418 @default.
- W2003619590 hasConcept C127413603 @default.
- W2003619590 hasConcept C149556010 @default.
- W2003619590 hasConcept C161790260 @default.
- W2003619590 hasConcept C166014724 @default.
- W2003619590 hasConcept C168170006 @default.
- W2003619590 hasConcept C178790620 @default.
- W2003619590 hasConcept C181199279 @default.
- W2003619590 hasConcept C184651966 @default.
- W2003619590 hasConcept C185592680 @default.
- W2003619590 hasConcept C191503008 @default.
- W2003619590 hasConcept C2777289219 @default.
- W2003619590 hasConcept C2780857362 @default.
- W2003619590 hasConcept C33633552 @default.
- W2003619590 hasConcept C42360764 @default.
- W2003619590 hasConcept C55493867 @default.
- W2003619590 hasConcept C65024703 @default.
- W2003619590 hasConcept C69118441 @default.
- W2003619590 hasConceptScore W2003619590C111368507 @default.
- W2003619590 hasConceptScore W2003619590C127313418 @default.
- W2003619590 hasConceptScore W2003619590C127413603 @default.
- W2003619590 hasConceptScore W2003619590C149556010 @default.
- W2003619590 hasConceptScore W2003619590C161790260 @default.
- W2003619590 hasConceptScore W2003619590C166014724 @default.
- W2003619590 hasConceptScore W2003619590C168170006 @default.
- W2003619590 hasConceptScore W2003619590C178790620 @default.
- W2003619590 hasConceptScore W2003619590C181199279 @default.
- W2003619590 hasConceptScore W2003619590C184651966 @default.
- W2003619590 hasConceptScore W2003619590C185592680 @default.
- W2003619590 hasConceptScore W2003619590C191503008 @default.
- W2003619590 hasConceptScore W2003619590C2777289219 @default.
- W2003619590 hasConceptScore W2003619590C2780857362 @default.
- W2003619590 hasConceptScore W2003619590C33633552 @default.
- W2003619590 hasConceptScore W2003619590C42360764 @default.
- W2003619590 hasConceptScore W2003619590C55493867 @default.
- W2003619590 hasConceptScore W2003619590C65024703 @default.
- W2003619590 hasConceptScore W2003619590C69118441 @default.
- W2003619590 hasIssue "5" @default.
- W2003619590 hasLocation W20036195901 @default.
- W2003619590 hasLocation W20036195902 @default.
- W2003619590 hasOpenAccess W2003619590 @default.
- W2003619590 hasPrimaryLocation W20036195901 @default.
- W2003619590 hasRelatedWork W1980352342 @default.
- W2003619590 hasRelatedWork W2003619590 @default.
- W2003619590 hasRelatedWork W2007996989 @default.
- W2003619590 hasRelatedWork W2051122974 @default.
- W2003619590 hasRelatedWork W2071731975 @default.
- W2003619590 hasRelatedWork W2981506549 @default.
- W2003619590 hasRelatedWork W3087653025 @default.
- W2003619590 hasRelatedWork W4205382136 @default.
- W2003619590 hasRelatedWork W4235432588 @default.
- W2003619590 hasRelatedWork W4385210523 @default.
- W2003619590 hasVolume "51" @default.
- W2003619590 isParatext "false" @default.
- W2003619590 isRetracted "false" @default.
- W2003619590 magId "2003619590" @default.
- W2003619590 workType "article" @default.