Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003627032> ?p ?o ?g. }
- W2003627032 endingPage "1384" @default.
- W2003627032 startingPage "1374" @default.
- W2003627032 abstract "Ex vivo analysis is an accurate and convenient way to study in vivo microglia phenotype and function. However, current microglia isolation protocols for ex vivo analysis show many differences in isolation steps (perfusion, removal of meninges and blood vessels, mechanical dissociation, enzymatic dissociation, density separation, immunomagnetic separation, and fluorescence-activated cell sorting), often without addressing their effects on microglia purity, number, phenotype, and function. Therefore, the aim of this study was to provide an optimized isolation protocol with emphasis on microglia purity and number to enable ex vivo analysis of adult mouse microglia. The application of this protocol for ex vivo phenotype and functional analysis is corroborated by results from flow cytometry, gene expression analysis, chemotaxis, and phagocytosis assays. In addition, this study shows the possibility to analyze microglia isolated from various central nervous system regions such as optic nerve, striatum, hippocampus, spinal cord, cerebellum, and cerebral cortex. Furthermore, this is the first study presenting DRAQ5 as a superior alternative to propidium iodide for the discrimination between living and dead cells. DRAQ5 staining facilitated the identification of microglia upon flow cytometry without the need of additional fluorescent markers. Along with a favorable emission spectrum, DRAQ5 proved a valuable tool for flow cytometry of microglia. The presented optimized microglia isolation protocol for ex vivo analysis offers the opportunity to obtain more insight into both general and region-specific microglia behavior." @default.
- W2003627032 created "2016-06-24" @default.
- W2003627032 creator A5014763110 @default.
- W2003627032 creator A5021110880 @default.
- W2003627032 creator A5034703266 @default.
- W2003627032 creator A5045617609 @default.
- W2003627032 date "2007-07-27" @default.
- W2003627032 modified "2023-09-27" @default.
- W2003627032 title "Optimized isolation enables ex vivo analysis of microglia from various central nervous system regions" @default.
- W2003627032 cites W1533029876 @default.
- W2003627032 cites W1540430336 @default.
- W2003627032 cites W1552686264 @default.
- W2003627032 cites W1575263086 @default.
- W2003627032 cites W175813982 @default.
- W2003627032 cites W1892094570 @default.
- W2003627032 cites W1931754955 @default.
- W2003627032 cites W1941031382 @default.
- W2003627032 cites W1947551305 @default.
- W2003627032 cites W1964445144 @default.
- W2003627032 cites W1971249131 @default.
- W2003627032 cites W1971751691 @default.
- W2003627032 cites W1972534236 @default.
- W2003627032 cites W1977604451 @default.
- W2003627032 cites W1978368004 @default.
- W2003627032 cites W1981310043 @default.
- W2003627032 cites W1985099526 @default.
- W2003627032 cites W1986830129 @default.
- W2003627032 cites W1989991560 @default.
- W2003627032 cites W1991805788 @default.
- W2003627032 cites W1991873176 @default.
- W2003627032 cites W1996605253 @default.
- W2003627032 cites W1998755771 @default.
- W2003627032 cites W2002267815 @default.
- W2003627032 cites W2004421590 @default.
- W2003627032 cites W2006117395 @default.
- W2003627032 cites W2009199474 @default.
- W2003627032 cites W2011221727 @default.
- W2003627032 cites W2013181947 @default.
- W2003627032 cites W2014704357 @default.
- W2003627032 cites W2015995193 @default.
- W2003627032 cites W2016260328 @default.
- W2003627032 cites W2017941203 @default.
- W2003627032 cites W2028989796 @default.
- W2003627032 cites W2035308998 @default.
- W2003627032 cites W2041796079 @default.
- W2003627032 cites W2045512359 @default.
- W2003627032 cites W2046096265 @default.
- W2003627032 cites W2050707975 @default.
- W2003627032 cites W2053313675 @default.
- W2003627032 cites W2060448382 @default.
- W2003627032 cites W2063249182 @default.
- W2003627032 cites W2066216713 @default.
- W2003627032 cites W2069814345 @default.
- W2003627032 cites W2074609120 @default.
- W2003627032 cites W2074736197 @default.
- W2003627032 cites W2077003881 @default.
- W2003627032 cites W2082351110 @default.
- W2003627032 cites W2082786396 @default.
- W2003627032 cites W2092749732 @default.
- W2003627032 cites W2093455511 @default.
- W2003627032 cites W2094660330 @default.
- W2003627032 cites W2096880268 @default.
- W2003627032 cites W2101853437 @default.
- W2003627032 cites W2107277218 @default.
- W2003627032 cites W2113107925 @default.
- W2003627032 cites W2116496066 @default.
- W2003627032 cites W2122828560 @default.
- W2003627032 cites W2124667416 @default.
- W2003627032 cites W2134769581 @default.
- W2003627032 cites W2144041807 @default.
- W2003627032 cites W2146631683 @default.
- W2003627032 cites W2148255040 @default.
- W2003627032 cites W2149548143 @default.
- W2003627032 cites W2156546316 @default.
- W2003627032 cites W2159372753 @default.
- W2003627032 cites W4244847225 @default.
- W2003627032 doi "https://doi.org/10.1002/glia.20554" @default.
- W2003627032 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17661344" @default.
- W2003627032 hasPublicationYear "2007" @default.
- W2003627032 type Work @default.
- W2003627032 sameAs 2003627032 @default.
- W2003627032 citedByCount "74" @default.
- W2003627032 countsByYear W20036270322012 @default.
- W2003627032 countsByYear W20036270322013 @default.
- W2003627032 countsByYear W20036270322014 @default.
- W2003627032 countsByYear W20036270322015 @default.
- W2003627032 countsByYear W20036270322016 @default.
- W2003627032 countsByYear W20036270322017 @default.
- W2003627032 countsByYear W20036270322018 @default.
- W2003627032 countsByYear W20036270322019 @default.
- W2003627032 countsByYear W20036270322020 @default.
- W2003627032 countsByYear W20036270322021 @default.
- W2003627032 countsByYear W20036270322022 @default.
- W2003627032 crossrefType "journal-article" @default.
- W2003627032 hasAuthorship W2003627032A5014763110 @default.
- W2003627032 hasAuthorship W2003627032A5021110880 @default.
- W2003627032 hasAuthorship W2003627032A5034703266 @default.
- W2003627032 hasAuthorship W2003627032A5045617609 @default.