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- W2003644928 abstract "The fumonisins, mycotoxins produced by Fusarium moniliforme, are known to occur as natural contaminants of corn worldwide and to be associated with several animal disease syndromes. High-performance liquid chromatography (HPLC) and monoclonal antibody-based (MAb) competitive direct enzyme-linked immunosorbent assay (CD-ELISA) methods, developed for the determination of fumonisins in corn, were compared. CD-ELISA results for naturally contaminated corn were consistently higher than corresponding HPLC results. Quantitative differences were reduced by decreasing the organic phase composition of the extract and by introducing a hexane partitioning step. The results were indicative of a possible lipid-based matrix effect, but when applied to fumonisin-free corn spiked with fumonisin levels ranging from 0.8 to 12.8 μg/g, analyses by both techniques were well correlated (r = 0.996). It is concluded that structurally related fumonisin-like compounds, present in naturally contaminated corn, may contribute to the differences recorded between the two methods, although the MAb-based CD-ELISA may still be used as an initial semiquantitative screening technique. Keywords: Fumonisins; immunoassay; ELISA; HPLC; corn" @default.
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- W2003644928 date "1996-01-01" @default.
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- W2003644928 title "Determination of Fumonisins in Corn: Evaluation of Competitive Immunoassay and HPLC Techniques" @default.
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- W2003644928 doi "https://doi.org/10.1021/jf950203k" @default.
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