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• W2003649493 abstract "Covalently attached organic monolayers on etched Si(1 1 1) surfaces were prepared by heating solutions of 1-alkenes and 1-alkynes in a refluxing mesitylene. Surface modification was monitored by measurement of the static water contact angle, X-ray photoelectron spectroscopy (XPS), infrared reflection absorption spectroscopy (IRRAS), and atomic force microscopy (AFM). Flat and clean N-hydroxysuccinimide (NHS)-ester-terminated/1-decyl mixed monolayers were covalently attached in one step onto a silicon surface. This procedure allows a mild and rapid functionalization of the surface by substitution of the NHS-ester moieties with amines at room temperature. The NHS-ester groups were shown to be fully intact onto the surface. The surface reactivity of the NHS-ester moieties toward amines was qualitatively and quantitatively evaluated via the reaction with methoxytetraethyleneglycolamine (TEGamine) and finally functionalized with single strand and complete DNA molecules. Moreover, domains of DNA were selectively immobilized, on silicon surface making use of TEGamine, which acts as protein repelling agent and therefore prevented non-specific DNA adsorption. The resulting DNA-modified surfaces have shown excellent specificity, and chemical and thermal stability under hybridization conditions." @default.
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• W2003649493 date "2011-03-01" @default.
• W2003649493 modified "2023-10-14" @default.
• W2003649493 title "A short route of covalent biofunctionaliztion of silicon surfaces" @default.
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• W2003649493 doi "https://doi.org/10.1016/j.snb.2010.12.013" @default.
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