FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003702370> ?p ?o ?g. }

• W2003702370 endingPage "820" @default.
• W2003702370 startingPage "813" @default.
• W2003702370 abstract "Signalling via the Notch receptor is a key regulator of many developmental processes. The differential responsiveness of Notch-expressing cells to the ligands Delta and Serrate is controlled by Fringe, itself essential for normal patterning in Drosophila and vertebrates. The mechanism of Fringe action, however, is not known. The protein has an amino-terminal hydrophobic stretch resembling a cleaved signal peptide, which has led to the widespread assumption that it is a secreted signalling molecule. It also has distant homology to bacterial glycosyltransferases, although it is not clear if this reflects a shared enzymatic activity, or merely a related structure.We report that a functional epitope-tagged form of Drosophila Fringe was localised in the Golgi apparatus. When the putative signal peptide was replaced by a confirmed one, Fringe no longer accumulated in the Golgi, but was instead efficiently secreted. This change in localisation dramatically reduced its biological activity, implying that the wild-type protein normally acts inside the cell. We show that Fringe specifically binds the nucleoside diphosphate UDP, a feature of many glycosyltransferases. Furthermore, specific mutation of a DxD motif (in the single-letter amino acid code where x is any amino acid), a hallmark of most glycosyltransferases that use nucleoside diphosphate sugars, did not affect the Golgi localisation of the protein but completely eliminated in vivo activity.These results indicate that Fringe does not exert its effects outside of the cell, but rather acts in the Golgi apparatus, apparently as a glycosyltransferase. They suggest that alteration in receptor glycosylation can regulate the relative efficiency of different ligands." @default.
• W2003702370 created "2016-06-24" @default.
• W2003702370 creator A5059008048 @default.
• W2003702370 creator A5077045618 @default.
• W2003702370 date "2000-07-01" @default.
• W2003702370 modified "2023-10-01" @default.
• W2003702370 title "The Notch signalling regulator Fringe acts in the Golgi apparatus and requires the glycosyltransferase signature motif DxD" @default.
• W2003702370 cites W1481480776 @default.
• W2003702370 cites W1491063745 @default.
• W2003702370 cites W1557651605 @default.
• W2003702370 cites W1606167220 @default.
• W2003702370 cites W1661341289 @default.
• W2003702370 cites W1805204704 @default.
• W2003702370 cites W1885556853 @default.
• W2003702370 cites W1912870130 @default.
• W2003702370 cites W1968146121 @default.
• W2003702370 cites W1975561755 @default.
• W2003702370 cites W1975771868 @default.
• W2003702370 cites W1976686299 @default.
• W2003702370 cites W1981792027 @default.
• W2003702370 cites W1982816926 @default.
• W2003702370 cites W1989339314 @default.
• W2003702370 cites W1990891647 @default.
• W2003702370 cites W1995717845 @default.
• W2003702370 cites W2015839491 @default.
• W2003702370 cites W2023496422 @default.
• W2003702370 cites W2029454635 @default.
• W2003702370 cites W2032157793 @default.
• W2003702370 cites W2035732431 @default.
• W2003702370 cites W2036312848 @default.
• W2003702370 cites W2040362901 @default.
• W2003702370 cites W2042956754 @default.
• W2003702370 cites W2045390772 @default.
• W2003702370 cites W2045790644 @default.
• W2003702370 cites W2053483926 @default.
• W2003702370 cites W2073877300 @default.
• W2003702370 cites W2077513922 @default.
• W2003702370 cites W2094690446 @default.
• W2003702370 cites W2110643873 @default.
• W2003702370 cites W2114643666 @default.
• W2003702370 cites W2114688668 @default.
• W2003702370 cites W2115710616 @default.
• W2003702370 cites W2116055030 @default.
• W2003702370 cites W2134349499 @default.
• W2003702370 cites W2135624706 @default.
• W2003702370 cites W2143275370 @default.
• W2003702370 cites W2143760153 @default.
• W2003702370 cites W2154861644 @default.
• W2003702370 cites W2163539354 @default.
• W2003702370 cites W2164898869 @default.
• W2003702370 cites W2170231361 @default.
• W2003702370 cites W2183722523 @default.
• W2003702370 cites W2187570082 @default.
• W2003702370 doi "https://doi.org/10.1016/s0960-9822(00)00578-9" @default.
• W2003702370 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10899003" @default.
• W2003702370 hasPublicationYear "2000" @default.
• W2003702370 type Work @default.
• W2003702370 sameAs 2003702370 @default.
• W2003702370 citedByCount "184" @default.
• W2003702370 countsByYear W20037023702012 @default.
• W2003702370 countsByYear W20037023702013 @default.
• W2003702370 countsByYear W20037023702014 @default.
• W2003702370 countsByYear W20037023702015 @default.
• W2003702370 countsByYear W20037023702016 @default.
• W2003702370 countsByYear W20037023702018 @default.
• W2003702370 countsByYear W20037023702019 @default.
• W2003702370 countsByYear W20037023702020 @default.
• W2003702370 countsByYear W20037023702021 @default.
• W2003702370 countsByYear W20037023702022 @default.
• W2003702370 countsByYear W20037023702023 @default.
• W2003702370 crossrefType "journal-article" @default.
• W2003702370 hasAuthorship W2003702370A5059008048 @default.
• W2003702370 hasAuthorship W2003702370A5077045618 @default.
• W2003702370 hasBestOaLocation W20037023701 @default.
• W2003702370 hasConcept C119062480 @default.
• W2003702370 hasConcept C158617107 @default.
• W2003702370 hasConcept C161019410 @default.
• W2003702370 hasConcept C181199279 @default.
• W2003702370 hasConcept C2777313579 @default.
• W2003702370 hasConcept C55493867 @default.
• W2003702370 hasConcept C86803240 @default.
• W2003702370 hasConcept C95444343 @default.
• W2003702370 hasConceptScore W2003702370C119062480 @default.
• W2003702370 hasConceptScore W2003702370C158617107 @default.
• W2003702370 hasConceptScore W2003702370C161019410 @default.
• W2003702370 hasConceptScore W2003702370C181199279 @default.
• W2003702370 hasConceptScore W2003702370C2777313579 @default.
• W2003702370 hasConceptScore W2003702370C55493867 @default.
• W2003702370 hasConceptScore W2003702370C86803240 @default.
• W2003702370 hasConceptScore W2003702370C95444343 @default.
• W2003702370 hasIssue "14" @default.
• W2003702370 hasLocation W20037023701 @default.
• W2003702370 hasLocation W20037023702 @default.
• W2003702370 hasOpenAccess W2003702370 @default.
• W2003702370 hasPrimaryLocation W20037023701 @default.
• W2003702370 hasRelatedWork W1967010451 @default.
• W2003702370 hasRelatedWork W1977927502 @default.
• W2003702370 hasRelatedWork W198820092 @default.

• next