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• W2003704973 abstract "Saccharomyces cerevisiae KAY446 was utilized for ethanol production, with glucose concentrations ranging from 120 g/L (normal) to 300 g/L (high). Although grown in a high glucose environment, S. cerevisiae still retained the ability to produce ethanol with a high degree of glucose utilization. iTRAQ-mediated shotgun proteomics was applied to identify relative expression change of proteins under the different glucose conditions. A total of 413 proteins were identified from three replicate, independent LC-MS/MS runs. Unsurprisingly, many proteins in the glycolysis/gluconeogenesis pathway showed significant changes in expression level. Twenty five proteins involved in amino acid metabolism decreased their expression, while the expressions of 12 heat-shock related proteins were also identified. Under high glucose conditions, ethanol was produced as a major product. However, the assimilation of glucose as well as a number of byproducts was also enhanced. Therefore, to optimize the ethanol production under very high gravity conditions, a number of pathways will need to be deactivated, while still maintaining the correct cellular redox or osmotic state. Proteomics is demonstrated here as a tool to aid in this forward metabolic engineering. Keywords: • S. cerevisiae • proteomics • ethanol production • high glucose concentration • iTRAQ • injection replicates" @default.
• W2003704973 created "2016-06-24" @default.
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• W2003704973 date "2006-11-07" @default.
• W2003704973 modified "2023-10-18" @default.
• W2003704973 title "Proteomic Analysis of <i>Saccharomyces cerevisiae</i> under High Gravity Fermentation Conditions" @default.
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• W2003704973 doi "https://doi.org/10.1021/pr060377p" @default.
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