FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003758281> ?p ?o ?g. }

• W2003758281 endingPage "325" @default.
• W2003758281 startingPage "318" @default.
• W2003758281 abstract "Hydrophobic substitutions at solvent-exposed positions in two α-helical regions of the bacteriophage P22 Arc repressor were introduced by combinatorial mutagenesis. In helix A, hydrophobic residues were tolerated individually at each of the five positions examined, but multiple substitutions were poorly tolerated as shown by the finding that mutants with more than two additional hydrophobic residues were biologically inactive. Several inactive helix A variants were purified and found to have reduced thermal stability relative to wild-type Arc, with a rough correlation between the number of polar-to-hydrophobic substitutions and the magnitude of the stability defect. Quite different results were obtained in helix B, where variants with as many as five polar-to-hydrophobic substitutions were found to be biologically active and one variant with three hydrophobic substitutions had a tm 6°C higher than wild-type. By contrast, a helix A mutant with three similar polar-to-hydrophobic substitutions was 23°C less stable than wild-type. Also, one set of three polar-to-hydrophobic substitutions in helix B was tolerated when introduced into the wild-type background but not when introduced into an equally active mutant having a nearly identical structure. Context effects occur both when comparing different regions of the same protein and when comparing the same region in two different homologues." @default.
• W2003758281 created "2016-06-24" @default.
• W2003758281 creator A5011979076 @default.
• W2003758281 creator A5072973603 @default.
• W2003758281 date "2008-12-31" @default.
• W2003758281 modified "2023-09-27" @default.
• W2003758281 title "Tolerance of a protein to multiple polar-to-hydrophobic surface substitutions" @default.
• W2003758281 cites W1523335288 @default.
• W2003758281 cites W1569553189 @default.
• W2003758281 cites W1964442246 @default.
• W2003758281 cites W1972216879 @default.
• W2003758281 cites W1987160823 @default.
• W2003758281 cites W2010260387 @default.
• W2003758281 cites W2023577097 @default.
• W2003758281 cites W2028597865 @default.
• W2003758281 cites W2029838687 @default.
• W2003758281 cites W2035407823 @default.
• W2003758281 cites W2036384511 @default.
• W2003758281 cites W2041658859 @default.
• W2003758281 cites W2049378642 @default.
• W2003758281 cites W2049981909 @default.
• W2003758281 cites W2060690425 @default.
• W2003758281 cites W2068265376 @default.
• W2003758281 cites W2075792028 @default.
• W2003758281 cites W2082523582 @default.
• W2003758281 cites W2088199349 @default.
• W2003758281 cites W2094503501 @default.
• W2003758281 cites W2100413315 @default.
• W2003758281 cites W2101381662 @default.
• W2003758281 cites W2114304598 @default.
• W2003758281 cites W2118456132 @default.
• W2003758281 doi "https://doi.org/10.1110/ps.8.2.318" @default.
• W2003758281 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2144263" @default.
• W2003758281 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10048325" @default.
• W2003758281 hasPublicationYear "2008" @default.
• W2003758281 type Work @default.
• W2003758281 sameAs 2003758281 @default.
• W2003758281 citedByCount "46" @default.
• W2003758281 countsByYear W20037582812012 @default.
• W2003758281 countsByYear W20037582812013 @default.
• W2003758281 countsByYear W20037582812014 @default.
• W2003758281 countsByYear W20037582812015 @default.
• W2003758281 countsByYear W20037582812016 @default.
• W2003758281 countsByYear W20037582812017 @default.
• W2003758281 countsByYear W20037582812018 @default.
• W2003758281 countsByYear W20037582812019 @default.
• W2003758281 countsByYear W20037582812023 @default.
• W2003758281 crossrefType "journal-article" @default.
• W2003758281 hasAuthorship W2003758281A5011979076 @default.
• W2003758281 hasAuthorship W2003758281A5072973603 @default.
• W2003758281 hasBestOaLocation W20037582812 @default.
• W2003758281 hasConcept C104317684 @default.
• W2003758281 hasConcept C121332964 @default.
• W2003758281 hasConcept C1276947 @default.
• W2003758281 hasConcept C143065580 @default.
• W2003758281 hasConcept C147816474 @default.
• W2003758281 hasConcept C151730666 @default.
• W2003758281 hasConcept C16318435 @default.
• W2003758281 hasConcept C181199279 @default.
• W2003758281 hasConcept C185592680 @default.
• W2003758281 hasConcept C18903297 @default.
• W2003758281 hasConcept C2778530040 @default.
• W2003758281 hasConcept C2779343474 @default.
• W2003758281 hasConcept C2779965526 @default.
• W2003758281 hasConcept C29705727 @default.
• W2003758281 hasConcept C55493867 @default.
• W2003758281 hasConcept C71240020 @default.
• W2003758281 hasConcept C8010536 @default.
• W2003758281 hasConcept C86803240 @default.
• W2003758281 hasConcept C90260826 @default.
• W2003758281 hasConceptScore W2003758281C104317684 @default.
• W2003758281 hasConceptScore W2003758281C121332964 @default.
• W2003758281 hasConceptScore W2003758281C1276947 @default.
• W2003758281 hasConceptScore W2003758281C143065580 @default.
• W2003758281 hasConceptScore W2003758281C147816474 @default.
• W2003758281 hasConceptScore W2003758281C151730666 @default.
• W2003758281 hasConceptScore W2003758281C16318435 @default.
• W2003758281 hasConceptScore W2003758281C181199279 @default.
• W2003758281 hasConceptScore W2003758281C185592680 @default.
• W2003758281 hasConceptScore W2003758281C18903297 @default.
• W2003758281 hasConceptScore W2003758281C2778530040 @default.
• W2003758281 hasConceptScore W2003758281C2779343474 @default.
• W2003758281 hasConceptScore W2003758281C2779965526 @default.
• W2003758281 hasConceptScore W2003758281C29705727 @default.
• W2003758281 hasConceptScore W2003758281C55493867 @default.
• W2003758281 hasConceptScore W2003758281C71240020 @default.
• W2003758281 hasConceptScore W2003758281C8010536 @default.
• W2003758281 hasConceptScore W2003758281C86803240 @default.
• W2003758281 hasConceptScore W2003758281C90260826 @default.
• W2003758281 hasIssue "2" @default.
• W2003758281 hasLocation W20037582811 @default.
• W2003758281 hasLocation W20037582812 @default.
• W2003758281 hasLocation W20037582813 @default.
• W2003758281 hasLocation W20037582814 @default.
• W2003758281 hasOpenAccess W2003758281 @default.
• W2003758281 hasPrimaryLocation W20037582811 @default.
• W2003758281 hasRelatedWork W1965342214 @default.
• W2003758281 hasRelatedWork W1980490260 @default.

• next