Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003770036> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2003770036 endingPage "233" @default.
- W2003770036 startingPage "227" @default.
- W2003770036 abstract "GPI-80 is a glycosylphosphatidylinositol (GPI)-anchored protein that is mainly expressed in human neutrophils. Previous studies using 3H9, a monoclonal antibody (mAb) against GPI-80, suggested that GPI-80 regulates leukocyte adherence and migration through Mac-1. GPI-80, which is anchored at the plasma membrane in resting neutrophils, moves into the pseudopodia and is released from activated human neutrophils. Here, we demonstrate that neutrophil activation affects GPI-80 dynamics using a new anti-GPI-80 mAb, designated 4D4, which is directed against the form of GPI-80 found on resting human neutrophils. Similar to 3H9, 4D4 influences Mac-1-dependent neutrophil adhesion. Treatment of purified GPI-80 with periodic acid and trypsin indicated that 3H9 and 4D4 recognize peptide and carbohydrate moieties, respectively. Stimulation with fMLP decreased the binding of 4D4 to GPI-80 on the neutrophil surface but increased the overall expression of GPI-80, as visualized by the 3H9 signal. Confocal laser microscopy revealed the 4D4 signal mainly on cell bodies and at a low level on pseudopodia during migration toward increasing concentrations of fMLP, whereas the 3H9 signal was observed in both areas. In addition, soluble GPI-80 released from activated neutrophils did not bind 4D4. These results suggest that there are two populations of GPI-80 that differ in the ability to bind 4D4. The 4D4-recognized form may regulate Mac-1-dependent neutrophil adhesion, and may subsequently be converted to a 4D4-unrecognized form during neutrophil activation." @default.
- W2003770036 created "2016-06-24" @default.
- W2003770036 creator A5009038415 @default.
- W2003770036 creator A5037153149 @default.
- W2003770036 creator A5041179989 @default.
- W2003770036 creator A5041660529 @default.
- W2003770036 creator A5051917586 @default.
- W2003770036 date "2007-07-01" @default.
- W2003770036 modified "2023-10-18" @default.
- W2003770036 title "Structural divergence of GPI-80 in activated human neutrophils" @default.
- W2003770036 cites W1547244189 @default.
- W2003770036 cites W1646222158 @default.
- W2003770036 cites W1970534801 @default.
- W2003770036 cites W1979681147 @default.
- W2003770036 cites W1993098240 @default.
- W2003770036 cites W1998131728 @default.
- W2003770036 cites W2029055174 @default.
- W2003770036 cites W2030446531 @default.
- W2003770036 cites W2031590808 @default.
- W2003770036 cites W2038187631 @default.
- W2003770036 cites W2054482958 @default.
- W2003770036 cites W2056447345 @default.
- W2003770036 cites W2079849822 @default.
- W2003770036 cites W2081065614 @default.
- W2003770036 cites W2092845660 @default.
- W2003770036 cites W2093536607 @default.
- W2003770036 cites W2135987235 @default.
- W2003770036 doi "https://doi.org/10.1016/j.bbrc.2007.05.087" @default.
- W2003770036 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17543284" @default.
- W2003770036 hasPublicationYear "2007" @default.
- W2003770036 type Work @default.
- W2003770036 sameAs 2003770036 @default.
- W2003770036 citedByCount "8" @default.
- W2003770036 countsByYear W20037700362013 @default.
- W2003770036 countsByYear W20037700362014 @default.
- W2003770036 countsByYear W20037700362016 @default.
- W2003770036 countsByYear W20037700362017 @default.
- W2003770036 countsByYear W20037700362018 @default.
- W2003770036 countsByYear W20037700362022 @default.
- W2003770036 crossrefType "journal-article" @default.
- W2003770036 hasAuthorship W2003770036A5009038415 @default.
- W2003770036 hasAuthorship W2003770036A5037153149 @default.
- W2003770036 hasAuthorship W2003770036A5041179989 @default.
- W2003770036 hasAuthorship W2003770036A5041660529 @default.
- W2003770036 hasAuthorship W2003770036A5051917586 @default.
- W2003770036 hasConcept C125705527 @default.
- W2003770036 hasConcept C134897094 @default.
- W2003770036 hasConcept C153911025 @default.
- W2003770036 hasConcept C159654299 @default.
- W2003770036 hasConcept C170493617 @default.
- W2003770036 hasConcept C178790620 @default.
- W2003770036 hasConcept C185592680 @default.
- W2003770036 hasConcept C199528912 @default.
- W2003770036 hasConcept C203014093 @default.
- W2003770036 hasConcept C2776914184 @default.
- W2003770036 hasConcept C54166955 @default.
- W2003770036 hasConcept C542903549 @default.
- W2003770036 hasConcept C55493867 @default.
- W2003770036 hasConcept C84416704 @default.
- W2003770036 hasConcept C86803240 @default.
- W2003770036 hasConcept C95444343 @default.
- W2003770036 hasConceptScore W2003770036C125705527 @default.
- W2003770036 hasConceptScore W2003770036C134897094 @default.
- W2003770036 hasConceptScore W2003770036C153911025 @default.
- W2003770036 hasConceptScore W2003770036C159654299 @default.
- W2003770036 hasConceptScore W2003770036C170493617 @default.
- W2003770036 hasConceptScore W2003770036C178790620 @default.
- W2003770036 hasConceptScore W2003770036C185592680 @default.
- W2003770036 hasConceptScore W2003770036C199528912 @default.
- W2003770036 hasConceptScore W2003770036C203014093 @default.
- W2003770036 hasConceptScore W2003770036C2776914184 @default.
- W2003770036 hasConceptScore W2003770036C54166955 @default.
- W2003770036 hasConceptScore W2003770036C542903549 @default.
- W2003770036 hasConceptScore W2003770036C55493867 @default.
- W2003770036 hasConceptScore W2003770036C84416704 @default.
- W2003770036 hasConceptScore W2003770036C86803240 @default.
- W2003770036 hasConceptScore W2003770036C95444343 @default.
- W2003770036 hasIssue "2" @default.
- W2003770036 hasLocation W20037700361 @default.
- W2003770036 hasLocation W20037700362 @default.
- W2003770036 hasOpenAccess W2003770036 @default.
- W2003770036 hasPrimaryLocation W20037700361 @default.
- W2003770036 hasRelatedWork W1990827051 @default.
- W2003770036 hasRelatedWork W1994281206 @default.
- W2003770036 hasRelatedWork W2021526434 @default.
- W2003770036 hasRelatedWork W2024882507 @default.
- W2003770036 hasRelatedWork W2107879108 @default.
- W2003770036 hasRelatedWork W2128775066 @default.
- W2003770036 hasRelatedWork W2142228914 @default.
- W2003770036 hasRelatedWork W2164386856 @default.
- W2003770036 hasRelatedWork W2583196190 @default.
- W2003770036 hasRelatedWork W3215646928 @default.
- W2003770036 hasVolume "359" @default.
- W2003770036 isParatext "false" @default.
- W2003770036 isRetracted "false" @default.
- W2003770036 magId "2003770036" @default.
- W2003770036 workType "article" @default.