Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003772532> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2003772532 endingPage "A172" @default.
- W2003772532 startingPage "A172" @default.
- W2003772532 abstract "Hydrolysis of glycyrrhizin (GL) to glycyrrhetic acid 3-O-mono-β-d-glucuronide (GAMG) by whole-cell biocatalysts in a system containing non-conventional solvents was performed. Three whole-cell biocatalysts were used, including wild-type Penicillium purpurogenum Li-3 (w-PGUS) and recombinant strains Escherichia coli BL21 and Pichia pastoris GS115. The biotransformation of GL to GAMG by w-PGUS in a 1-butyl-3-methylimidazolium hexafluorophosphate ([Bmim]PF6)/buffer biphasic system was the main focus of this study because w-PGUS showed a higher GAMG yield and a higher relative activity in this system than the other two whole-cell biocatalysts. Using the optimized reaction conditions determined as a pH 5.2 buffer, a 6.0 mM substrate concentration, a reaction temperature of 30 °C, and a 60 g/L (1.23 U/g) cell concentration, a GAMG yield of 87.63% was achieved after 60 h. After eight reaction cycles, [Bmim]PF6 retained a high recovery percentage (85.48%)[0], indicating the reusability of this IL. The biotransformation activity of w-PGUS was not significantly affected, even after two batch reaction cycles. Furthermore, the product GAMG and the byproduct glycyrrhetinic acid were spontaneously separated in the biphasic system. In conclusion, the combination of whole cells and ionic liquid is a promising approach for economical and industrial-scale production of GAMG." @default.
- W2003772532 created "2016-06-24" @default.
- W2003772532 creator A5031762844 @default.
- W2003772532 date "2012-01-01" @default.
- W2003772532 modified "2023-09-30" @default.
- W2003772532 title "Efficacité de l’éducation thérapeutique dans la BPCO : difficultés d’un essai randomisé" @default.
- W2003772532 doi "https://doi.org/10.1016/j.rmr.2011.10.806" @default.
- W2003772532 hasPublicationYear "2012" @default.
- W2003772532 type Work @default.
- W2003772532 sameAs 2003772532 @default.
- W2003772532 citedByCount "0" @default.
- W2003772532 crossrefType "journal-article" @default.
- W2003772532 hasAuthorship W2003772532A5031762844 @default.
- W2003772532 hasConcept C104317684 @default.
- W2003772532 hasConcept C134121241 @default.
- W2003772532 hasConcept C181199279 @default.
- W2003772532 hasConcept C185592680 @default.
- W2003772532 hasConcept C18903297 @default.
- W2003772532 hasConcept C191897082 @default.
- W2003772532 hasConcept C192562407 @default.
- W2003772532 hasConcept C2777289219 @default.
- W2003772532 hasConcept C2779222958 @default.
- W2003772532 hasConcept C27881333 @default.
- W2003772532 hasConcept C43617362 @default.
- W2003772532 hasConcept C547475151 @default.
- W2003772532 hasConcept C55493867 @default.
- W2003772532 hasConcept C86803240 @default.
- W2003772532 hasConcept C94412978 @default.
- W2003772532 hasConceptScore W2003772532C104317684 @default.
- W2003772532 hasConceptScore W2003772532C134121241 @default.
- W2003772532 hasConceptScore W2003772532C181199279 @default.
- W2003772532 hasConceptScore W2003772532C185592680 @default.
- W2003772532 hasConceptScore W2003772532C18903297 @default.
- W2003772532 hasConceptScore W2003772532C191897082 @default.
- W2003772532 hasConceptScore W2003772532C192562407 @default.
- W2003772532 hasConceptScore W2003772532C2777289219 @default.
- W2003772532 hasConceptScore W2003772532C2779222958 @default.
- W2003772532 hasConceptScore W2003772532C27881333 @default.
- W2003772532 hasConceptScore W2003772532C43617362 @default.
- W2003772532 hasConceptScore W2003772532C547475151 @default.
- W2003772532 hasConceptScore W2003772532C55493867 @default.
- W2003772532 hasConceptScore W2003772532C86803240 @default.
- W2003772532 hasConceptScore W2003772532C94412978 @default.
- W2003772532 hasLocation W20037725321 @default.
- W2003772532 hasOpenAccess W2003772532 @default.
- W2003772532 hasPrimaryLocation W20037725321 @default.
- W2003772532 hasRelatedWork W2072332230 @default.
- W2003772532 hasRelatedWork W2076652852 @default.
- W2003772532 hasRelatedWork W2102214498 @default.
- W2003772532 hasRelatedWork W2352989805 @default.
- W2003772532 hasRelatedWork W2357612444 @default.
- W2003772532 hasRelatedWork W2361626110 @default.
- W2003772532 hasRelatedWork W2363316691 @default.
- W2003772532 hasRelatedWork W2374537212 @default.
- W2003772532 hasRelatedWork W2375169699 @default.
- W2003772532 hasRelatedWork W2972139489 @default.
- W2003772532 hasVolume "29" @default.
- W2003772532 isParatext "false" @default.
- W2003772532 isRetracted "false" @default.
- W2003772532 magId "2003772532" @default.
- W2003772532 workType "article" @default.