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• W2003805523 endingPage "758" @default.
• W2003805523 startingPage "750" @default.
• W2003805523 abstract "Abstract The role of fructose-2,6-bisphosphate (Fru-2,6-P2) as a regulatory metabolite in photosynthetic carbohydrate metabolism was studied in transgenic Arabidopsis plants with reduced activity of Fru-6-phosphate,2-kinase/Fru-2,6-bisphosphatase. A positive correlation was observed between the Fru-6-phosphate,2-kinase activity and the level of Fru-2,6-P2 in the leaves. The partitioning of carbon was studied by 14CO2 labeling of photosynthetic products. Plant lines with Fru-2,6-P2 levels down to 5% of the levels observed in wild-type (WT) plants had significantly altered partitioning of carbon between sucrose (Suc) versus starch. The ratio of 14C incorporated into Suc and starch increased 2- to 3-fold in the plants with low levels of Fru-2,6-P2 compared with WT. Transgenic plant lines with intermediate levels of Fru-2,6-P2 compared with WT had a Suc-to-starch labeling ratio similar to the WT. Levels of sugars, starch, and phosphorylated intermediates in leaves were followed during the diurnal cycle. Plants with low levels of Fru-2,6-P2 in leaves had high levels of Suc, glucose, and Fru and low levels of triose phosphates and glucose-1-P during the light period compared with WT. During the dark period these differences were eliminated. Our data provide direct evidence that Fru-2,6-P2 affects photosynthetic carbon partitioning in Arabidopsis. Opposed to this, Fru-2,6-P2 does not contribute significantly to regulation of metabolite levels in darkness." @default.
• W2003805523 created "2016-06-24" @default.
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• W2003805523 date "2001-06-01" @default.
• W2003805523 modified "2023-09-27" @default.
• W2003805523 title "Transgenic Arabidopsis Plants with Decreased Activity of Fructose-6-Phosphate,2-Kinase/Fructose-2,6-Bisphosphatase Have Altered Carbon Partitioning" @default.
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• W2003805523 doi "https://doi.org/10.1104/pp.126.2.750" @default.
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