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• W2003809982 abstract "Parotid gland acinar cells undergo marked hypertrophy and hyperplasia upon systemic exposure to the β-adrenergic agonist, isoproterenol. This glandular enlargement is accompanied by substantial cellular changes including DNA synthesis, an increase in glandular protein synthesis, and differential changes in RNA transcription. To gain a more detailed understanding of the underlying changes induced by isoproterenol, we have examined the parotid gland gene expression profile of mice up to 24 h post-isoproterenol injection using high-density oligonucleotide arrays. Depending upon the exposure time, between 22 and 48 of the ∼6,500 mouse genes and expressed sequence tags (ESTs) analyzed displayed significant changes in expression patterns. Genes that were previously shown to be repressed (α-amylase) or activated (proline-rich proteins) following isoproterenol exposure were found to be similarly affected in this experiment, validating this technique. This study demonstrates that the oligonucleotide array technology is a useful tool for examining isoproterenol-induced salivary gland gene expression changes. Using this as a starting point, we can begin to dissect the specific pathways involved in mediating isoproterenol action within the parotid gland." @default.
• W2003809982 created "2016-06-24" @default.
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• W2003809982 date "2002-02-28" @default.
• W2003809982 modified "2023-10-06" @default.
• W2003809982 title "Analysis of isoproterenol-induced changes in parotid gland gene expression" @default.
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• W2003809982 doi "https://doi.org/10.1152/physiolgenomics.00039.2001" @default.
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