Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003827864> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2003827864 endingPage "9" @default.
- W2003827864 startingPage "3" @default.
- W2003827864 abstract "Numerous RNA species of different length and nucleotide sequence grow spontaneously in vitro in Q beta replicase reactions where no RNA templates are added deliberately. Here, we show that this spontaneous RNA synthesis by Q beta replicase is template directed. The immediate source of template RNA can be the laboratory air, but there are ways to eliminate, or at least substantially reduce, the harmful effects of spontaneous synthesis. Solitary RNA molecules were detected in a thin layer of agarose gel containing Q beta replicase, where they grew to form colonies that became visible upon staining with ethidium bromide. This result provides a powerful tool for RNA cloning and selection in vitro. We also show that replicating RNAs similar to those growing spontaneously are incorporated into Q beta phage particles and can propagate in vivo for a number of phage generations. These RNAs are the smallest known molecular parasites, and in many aspects they resemble both the defective interfering genomes of animal and plant viruses and plant virus satellite RNAs." @default.
- W2003827864 created "2016-06-24" @default.
- W2003827864 creator A5066311509 @default.
- W2003827864 creator A5078506573 @default.
- W2003827864 creator A5089790993 @default.
- W2003827864 date "1991-11-01" @default.
- W2003827864 modified "2023-10-13" @default.
- W2003827864 title "On the nature of spontaneous RNA synthesis by Qβ replicase" @default.
- W2003827864 cites W1544392112 @default.
- W2003827864 cites W1597135489 @default.
- W2003827864 cites W1897115139 @default.
- W2003827864 cites W1966144319 @default.
- W2003827864 cites W1991418592 @default.
- W2003827864 cites W1993486727 @default.
- W2003827864 cites W1999640036 @default.
- W2003827864 cites W2002095302 @default.
- W2003827864 cites W2007029862 @default.
- W2003827864 cites W2024093259 @default.
- W2003827864 cites W2028808822 @default.
- W2003827864 cites W2030171801 @default.
- W2003827864 cites W2030727783 @default.
- W2003827864 cites W2044078869 @default.
- W2003827864 cites W2051302798 @default.
- W2003827864 cites W2054516546 @default.
- W2003827864 cites W2066836230 @default.
- W2003827864 cites W2074224507 @default.
- W2003827864 cites W2079778014 @default.
- W2003827864 cites W2080844835 @default.
- W2003827864 cites W2082497938 @default.
- W2003827864 cites W2084201875 @default.
- W2003827864 cites W2106011441 @default.
- W2003827864 cites W2107688779 @default.
- W2003827864 cites W2166284240 @default.
- W2003827864 cites W2205516038 @default.
- W2003827864 cites W2983539929 @default.
- W2003827864 doi "https://doi.org/10.1016/0022-2836(91)90729-p" @default.
- W2003827864 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1719219" @default.
- W2003827864 hasPublicationYear "1991" @default.
- W2003827864 type Work @default.
- W2003827864 sameAs 2003827864 @default.
- W2003827864 citedByCount "62" @default.
- W2003827864 countsByYear W20038278642013 @default.
- W2003827864 countsByYear W20038278642014 @default.
- W2003827864 countsByYear W20038278642016 @default.
- W2003827864 countsByYear W20038278642018 @default.
- W2003827864 countsByYear W20038278642019 @default.
- W2003827864 countsByYear W20038278642021 @default.
- W2003827864 countsByYear W20038278642022 @default.
- W2003827864 crossrefType "journal-article" @default.
- W2003827864 hasAuthorship W2003827864A5066311509 @default.
- W2003827864 hasAuthorship W2003827864A5078506573 @default.
- W2003827864 hasAuthorship W2003827864A5089790993 @default.
- W2003827864 hasConcept C104317684 @default.
- W2003827864 hasConcept C153911025 @default.
- W2003827864 hasConcept C159047783 @default.
- W2003827864 hasConcept C172827444 @default.
- W2003827864 hasConcept C194993378 @default.
- W2003827864 hasConcept C2776907736 @default.
- W2003827864 hasConcept C41258723 @default.
- W2003827864 hasConcept C54355233 @default.
- W2003827864 hasConcept C552990157 @default.
- W2003827864 hasConcept C67705224 @default.
- W2003827864 hasConcept C70721500 @default.
- W2003827864 hasConcept C86803240 @default.
- W2003827864 hasConceptScore W2003827864C104317684 @default.
- W2003827864 hasConceptScore W2003827864C153911025 @default.
- W2003827864 hasConceptScore W2003827864C159047783 @default.
- W2003827864 hasConceptScore W2003827864C172827444 @default.
- W2003827864 hasConceptScore W2003827864C194993378 @default.
- W2003827864 hasConceptScore W2003827864C2776907736 @default.
- W2003827864 hasConceptScore W2003827864C41258723 @default.
- W2003827864 hasConceptScore W2003827864C54355233 @default.
- W2003827864 hasConceptScore W2003827864C552990157 @default.
- W2003827864 hasConceptScore W2003827864C67705224 @default.
- W2003827864 hasConceptScore W2003827864C70721500 @default.
- W2003827864 hasConceptScore W2003827864C86803240 @default.
- W2003827864 hasIssue "1" @default.
- W2003827864 hasLocation W20038278641 @default.
- W2003827864 hasLocation W20038278642 @default.
- W2003827864 hasOpenAccess W2003827864 @default.
- W2003827864 hasPrimaryLocation W20038278641 @default.
- W2003827864 hasRelatedWork W1988125422 @default.
- W2003827864 hasRelatedWork W1999640036 @default.
- W2003827864 hasRelatedWork W2002930051 @default.
- W2003827864 hasRelatedWork W2038243974 @default.
- W2003827864 hasRelatedWork W2045939456 @default.
- W2003827864 hasRelatedWork W2058984380 @default.
- W2003827864 hasRelatedWork W2106687784 @default.
- W2003827864 hasRelatedWork W2123268304 @default.
- W2003827864 hasRelatedWork W2317467025 @default.
- W2003827864 hasRelatedWork W2900182103 @default.
- W2003827864 hasVolume "222" @default.
- W2003827864 isParatext "false" @default.
- W2003827864 isRetracted "false" @default.
- W2003827864 magId "2003827864" @default.
- W2003827864 workType "article" @default.