Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003843674> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2003843674 endingPage "2311" @default.
- W2003843674 startingPage "2308" @default.
- W2003843674 abstract "It is well known that it would be important to cultivate human hepatocytes of about 10(10) cells at a high cell density, about 1 x 10(7) cells/cm(3), in the bioreactor for the development of bioartificial liver. However, since primary human hepatocytes lack an ability to proliferate in vitro, it is essential to establish a culture method for the proliferation of normal human hepatic stem cells as a cell source. In this study, it was found that human hepatoblasts, a kind of hepatic stem cells, were induced from human fetal hepatocytes while keeping the ability of proliferation by the treatment of 1mM sodium butyrate (SB) for 12 d of culture. The transformation of hepatoblasts was evaluated by abnormal prothrombin (PIVKA-II) assay, which is a clinical marker for hepatocellular carcinoma. The PIVKA-II production rate of the cells was suppressed to the normal level under 1 mM SB. The cells including hepatoblasts under 1 mM SB attached to the porous hydroxyapatite carriers and proliferated to a high cell density of about 1 x 10(7) cells/cm(3) in the carriers. The liver-specific function, cytochrome P450 3A4 activity (4.2 pmol/mg protein/min) of the cells in the carriers under 1 mM SB was comparable to that of primary human hepatocytes. Ammonia metabolizing activity (0.21 micromol/10(6) cells/h) of the cells was also comparable to that of porcine hepatocytes used in the bioartificial liver. The PIVKA-II production rate of the cells in the carrier was suppressed to the normal level. These results suggested that induction of human hepatoblasts from fetal hepatocytes by the treatment of 1mM SB and proliferation of the cells at a high cell density using hydroxyapatite carriers should be one of the more promising culture methods for bioartificial liver developments." @default.
- W2003843674 created "2016-06-24" @default.
- W2003843674 creator A5000528022 @default.
- W2003843674 creator A5068896247 @default.
- W2003843674 creator A5070351307 @default.
- W2003843674 date "2007-01-01" @default.
- W2003843674 modified "2023-09-26" @default.
- W2003843674 title "Induction and High Density Culture of Human Hepatoblasts from Fetal Hepatocytes with Suppressing Transformation" @default.
- W2003843674 cites W1546331692 @default.
- W2003843674 cites W1967414895 @default.
- W2003843674 cites W1984010318 @default.
- W2003843674 cites W1999140812 @default.
- W2003843674 cites W2005325337 @default.
- W2003843674 cites W2012871544 @default.
- W2003843674 cites W2029446157 @default.
- W2003843674 cites W2030607025 @default.
- W2003843674 cites W2053810332 @default.
- W2003843674 cites W2065796189 @default.
- W2003843674 cites W2097280897 @default.
- W2003843674 cites W2120352389 @default.
- W2003843674 cites W2140937256 @default.
- W2003843674 cites W2330134488 @default.
- W2003843674 cites W2419793573 @default.
- W2003843674 cites W2491004180 @default.
- W2003843674 cites W350594800 @default.
- W2003843674 doi "https://doi.org/10.1248/bpb.30.2308" @default.
- W2003843674 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18057717" @default.
- W2003843674 hasPublicationYear "2007" @default.
- W2003843674 type Work @default.
- W2003843674 sameAs 2003843674 @default.
- W2003843674 citedByCount "13" @default.
- W2003843674 countsByYear W20038436742012 @default.
- W2003843674 countsByYear W20038436742013 @default.
- W2003843674 countsByYear W20038436742014 @default.
- W2003843674 countsByYear W20038436742015 @default.
- W2003843674 countsByYear W20038436742016 @default.
- W2003843674 countsByYear W20038436742021 @default.
- W2003843674 crossrefType "journal-article" @default.
- W2003843674 hasAuthorship W2003843674A5000528022 @default.
- W2003843674 hasAuthorship W2003843674A5068896247 @default.
- W2003843674 hasAuthorship W2003843674A5070351307 @default.
- W2003843674 hasBestOaLocation W20038436741 @default.
- W2003843674 hasConcept C1491633281 @default.
- W2003843674 hasConcept C153911025 @default.
- W2003843674 hasConcept C185592680 @default.
- W2003843674 hasConcept C202751555 @default.
- W2003843674 hasConcept C2776200302 @default.
- W2003843674 hasConcept C2778942008 @default.
- W2003843674 hasConcept C28328180 @default.
- W2003843674 hasConcept C54355233 @default.
- W2003843674 hasConcept C55493867 @default.
- W2003843674 hasConcept C62112901 @default.
- W2003843674 hasConcept C71867830 @default.
- W2003843674 hasConcept C81885089 @default.
- W2003843674 hasConcept C86803240 @default.
- W2003843674 hasConcept C95444343 @default.
- W2003843674 hasConceptScore W2003843674C1491633281 @default.
- W2003843674 hasConceptScore W2003843674C153911025 @default.
- W2003843674 hasConceptScore W2003843674C185592680 @default.
- W2003843674 hasConceptScore W2003843674C202751555 @default.
- W2003843674 hasConceptScore W2003843674C2776200302 @default.
- W2003843674 hasConceptScore W2003843674C2778942008 @default.
- W2003843674 hasConceptScore W2003843674C28328180 @default.
- W2003843674 hasConceptScore W2003843674C54355233 @default.
- W2003843674 hasConceptScore W2003843674C55493867 @default.
- W2003843674 hasConceptScore W2003843674C62112901 @default.
- W2003843674 hasConceptScore W2003843674C71867830 @default.
- W2003843674 hasConceptScore W2003843674C81885089 @default.
- W2003843674 hasConceptScore W2003843674C86803240 @default.
- W2003843674 hasConceptScore W2003843674C95444343 @default.
- W2003843674 hasIssue "12" @default.
- W2003843674 hasLocation W20038436741 @default.
- W2003843674 hasLocation W20038436742 @default.
- W2003843674 hasOpenAccess W2003843674 @default.
- W2003843674 hasPrimaryLocation W20038436741 @default.
- W2003843674 hasRelatedWork W196370084 @default.
- W2003843674 hasRelatedWork W1981686547 @default.
- W2003843674 hasRelatedWork W2014534222 @default.
- W2003843674 hasRelatedWork W2032209516 @default.
- W2003843674 hasRelatedWork W2083693850 @default.
- W2003843674 hasRelatedWork W2379833382 @default.
- W2003843674 hasRelatedWork W2406842358 @default.
- W2003843674 hasRelatedWork W2413532914 @default.
- W2003843674 hasRelatedWork W2442207534 @default.
- W2003843674 hasRelatedWork W3029744252 @default.
- W2003843674 hasVolume "30" @default.
- W2003843674 isParatext "false" @default.
- W2003843674 isRetracted "false" @default.
- W2003843674 magId "2003843674" @default.
- W2003843674 workType "article" @default.