Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003854865> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2003854865 abstract "Heterogeneous breast carcinomas often contain nuclei with multiple distributions of ploidy, each representing a distinct genomic subpopulation. We present an approach called Ploidy-Seq that combines flow-sorting and next-generation sequencing to isolate tumor subpopulations and deep-sequence the nuclei at high coverage (80-100X) to identify somatic mutations. We applied Ploidy-Seq to a triple-negative (ER-, PR-, Her2-) ductal carcinoma that showed three major tumor subpopulations and one stromal subpopulation. In order to distinguish germline from somatic mutations, we first identified all of the SNPs in the 2N stromal cells. We then studied the evolution of different molecular clocks within the breast tumor, including single nucleotide variations (SNVs), indels, structural rearrangements, copy number changes and translocations. By applying set theory operations, we compared the three tumor subpopulations to identify early mutations (present in all subpopulations), intermediate events (shared between two subpopulations) and late mutations (exclusive to each subpopulation). Overall we identified 101 nonsynonymous point mutations of which 31 had occurred early and were present in all tumor subpopulations. Many of the early mutations occurred in cancer genes including THSD7B involved in TGFβ signaling, the CDH26 cadherin-like 26 gene, and the kinesin gene KIF2B. Early mutations were further studied by single-cell sequencing, which showed evidence of clonal expansions within the tumor, suggesting that they provided a major selective advantage. These early mutations are of considerable interest for therapeutic targeting since they are present in all of the tumor subpopulations, and are likely to be driving the growth of the cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5054. doi:1538-7445.AM2012-5054" @default.
- W2003854865 created "2016-06-24" @default.
- W2003854865 creator A5026920706 @default.
- W2003854865 creator A5051190380 @default.
- W2003854865 creator A5059676911 @default.
- W2003854865 creator A5063693068 @default.
- W2003854865 creator A5064488370 @default.
- W2003854865 creator A5066102428 @default.
- W2003854865 creator A5079803961 @default.
- W2003854865 date "2012-04-15" @default.
- W2003854865 modified "2023-09-26" @default.
- W2003854865 title "Abstract 5054: Inferring mutational chronology in breast cancer by deep-sequencing tumor subpopulations" @default.
- W2003854865 doi "https://doi.org/10.1158/1538-7445.am2012-5054" @default.
- W2003854865 hasPublicationYear "2012" @default.
- W2003854865 type Work @default.
- W2003854865 sameAs 2003854865 @default.
- W2003854865 citedByCount "0" @default.
- W2003854865 crossrefType "proceedings-article" @default.
- W2003854865 hasAuthorship W2003854865A5026920706 @default.
- W2003854865 hasAuthorship W2003854865A5051190380 @default.
- W2003854865 hasAuthorship W2003854865A5059676911 @default.
- W2003854865 hasAuthorship W2003854865A5063693068 @default.
- W2003854865 hasAuthorship W2003854865A5064488370 @default.
- W2003854865 hasAuthorship W2003854865A5066102428 @default.
- W2003854865 hasAuthorship W2003854865A5079803961 @default.
- W2003854865 hasConcept C104317684 @default.
- W2003854865 hasConcept C113968399 @default.
- W2003854865 hasConcept C119054055 @default.
- W2003854865 hasConcept C120821319 @default.
- W2003854865 hasConcept C121608353 @default.
- W2003854865 hasConcept C132917006 @default.
- W2003854865 hasConcept C134305767 @default.
- W2003854865 hasConcept C135763542 @default.
- W2003854865 hasConcept C141231307 @default.
- W2003854865 hasConcept C149904235 @default.
- W2003854865 hasConcept C153209595 @default.
- W2003854865 hasConcept C166608930 @default.
- W2003854865 hasConcept C502942594 @default.
- W2003854865 hasConcept C530470458 @default.
- W2003854865 hasConcept C54355233 @default.
- W2003854865 hasConcept C86803240 @default.
- W2003854865 hasConceptScore W2003854865C104317684 @default.
- W2003854865 hasConceptScore W2003854865C113968399 @default.
- W2003854865 hasConceptScore W2003854865C119054055 @default.
- W2003854865 hasConceptScore W2003854865C120821319 @default.
- W2003854865 hasConceptScore W2003854865C121608353 @default.
- W2003854865 hasConceptScore W2003854865C132917006 @default.
- W2003854865 hasConceptScore W2003854865C134305767 @default.
- W2003854865 hasConceptScore W2003854865C135763542 @default.
- W2003854865 hasConceptScore W2003854865C141231307 @default.
- W2003854865 hasConceptScore W2003854865C149904235 @default.
- W2003854865 hasConceptScore W2003854865C153209595 @default.
- W2003854865 hasConceptScore W2003854865C166608930 @default.
- W2003854865 hasConceptScore W2003854865C502942594 @default.
- W2003854865 hasConceptScore W2003854865C530470458 @default.
- W2003854865 hasConceptScore W2003854865C54355233 @default.
- W2003854865 hasConceptScore W2003854865C86803240 @default.
- W2003854865 hasLocation W20038548651 @default.
- W2003854865 hasOpenAccess W2003854865 @default.
- W2003854865 hasPrimaryLocation W20038548651 @default.
- W2003854865 hasRelatedWork W1535686168 @default.
- W2003854865 hasRelatedWork W1974808932 @default.
- W2003854865 hasRelatedWork W2000905326 @default.
- W2003854865 hasRelatedWork W2022634109 @default.
- W2003854865 hasRelatedWork W2071480068 @default.
- W2003854865 hasRelatedWork W2112997276 @default.
- W2003854865 hasRelatedWork W2131592364 @default.
- W2003854865 hasRelatedWork W2157423592 @default.
- W2003854865 hasRelatedWork W2314118888 @default.
- W2003854865 hasRelatedWork W2314557879 @default.
- W2003854865 hasRelatedWork W2322746471 @default.
- W2003854865 hasRelatedWork W2335647268 @default.
- W2003854865 hasRelatedWork W2480740297 @default.
- W2003854865 hasRelatedWork W2566249167 @default.
- W2003854865 hasRelatedWork W2801176791 @default.
- W2003854865 hasRelatedWork W2803418544 @default.
- W2003854865 hasRelatedWork W2896930952 @default.
- W2003854865 hasRelatedWork W2955055779 @default.
- W2003854865 hasRelatedWork W3036176609 @default.
- W2003854865 hasRelatedWork W3047006373 @default.
- W2003854865 isParatext "false" @default.
- W2003854865 isRetracted "false" @default.
- W2003854865 magId "2003854865" @default.
- W2003854865 workType "article" @default.