Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003895100> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W2003895100 endingPage "98" @default.
- W2003895100 startingPage "93" @default.
- W2003895100 abstract "Abstract Medaka ( Oryzias latipes ), a fish model for assessing the carcinogenic impact of environmental exposure to genotoxic chemicals, were subjected to 390 ppm of diethylnitrosamine (DEN) in an aqueous bath for 48 h. Histological examinations, at 5 and 7 months post-exposure, revealed that 11 of 17 fish developed hepatic neoplasms (hepatocellular carcinomas, cholangiomas and mixed hepato-cholangiocellular carcinomas). Genomic DNA extracted from the livers of normal medaka was used to obtain and sequence the structure of ras gene exon 1 and exon 2. Comparing PCR-amplified products from exon 1 and 2 by hybridization with specific mutation containing probes and/or by sequencing of liver DNA from control and exposed fish revealed an association of ras oncogene mutations and DEN-induced tumor formation. While none of the control fish showed any mutations, three of 11 fish with tumors had ras gene mutations. All three fish had mutations at codon 12 replacing glycine with aspartic acid, while one fish had an additional mutation at codon 16 replacing lysine with threonine. In addition to identifying DEN-induced mutations at codon 12 of the medaka ras oncogene, the present study represents a first report of an in vivo association of hepatocarcinogenesis, in any species, with a mutation at codon 16. Mutation at codon 16 and tumorigenesis has been previously predicted and artificially produced. Our study also supports the relevance of the medaka fish model for in vivo detection of the carcinogenic potential of environmental pollutants in sediments and biota of ambient water systems." @default.
- W2003895100 created "2016-06-24" @default.
- W2003895100 creator A5042764284 @default.
- W2003895100 creator A5063514822 @default.
- W2003895100 creator A5071504404 @default.
- W2003895100 creator A5077931403 @default.
- W2003895100 creator A5085872479 @default.
- W2003895100 date "1996-06-01" @default.
- W2003895100 modified "2023-09-26" @default.
- W2003895100 title "Induction of ras oncogene mutations and hepatocarcinogenesis in medaka (Oryzias latipes) exposed to diethylnitrosamine" @default.
- W2003895100 cites W1978630371 @default.
- W2003895100 cites W1994846495 @default.
- W2003895100 cites W1999678934 @default.
- W2003895100 cites W2024084862 @default.
- W2003895100 cites W2024442749 @default.
- W2003895100 cites W2033228616 @default.
- W2003895100 cites W2037054090 @default.
- W2003895100 cites W2071249971 @default.
- W2003895100 cites W2075094795 @default.
- W2003895100 cites W2083363203 @default.
- W2003895100 cites W2090305138 @default.
- W2003895100 cites W2101619466 @default.
- W2003895100 cites W2127129115 @default.
- W2003895100 cites W2332547703 @default.
- W2003895100 doi "https://doi.org/10.1016/0141-1136(95)00051-8" @default.
- W2003895100 hasPublicationYear "1996" @default.
- W2003895100 type Work @default.
- W2003895100 sameAs 2003895100 @default.
- W2003895100 citedByCount "8" @default.
- W2003895100 crossrefType "journal-article" @default.
- W2003895100 hasAuthorship W2003895100A5042764284 @default.
- W2003895100 hasAuthorship W2003895100A5063514822 @default.
- W2003895100 hasAuthorship W2003895100A5071504404 @default.
- W2003895100 hasAuthorship W2003895100A5077931403 @default.
- W2003895100 hasAuthorship W2003895100A5085872479 @default.
- W2003895100 hasConcept C104317684 @default.
- W2003895100 hasConcept C114246631 @default.
- W2003895100 hasConcept C2777289358 @default.
- W2003895100 hasConcept C502942594 @default.
- W2003895100 hasConcept C54355233 @default.
- W2003895100 hasConcept C86803240 @default.
- W2003895100 hasConceptScore W2003895100C104317684 @default.
- W2003895100 hasConceptScore W2003895100C114246631 @default.
- W2003895100 hasConceptScore W2003895100C2777289358 @default.
- W2003895100 hasConceptScore W2003895100C502942594 @default.
- W2003895100 hasConceptScore W2003895100C54355233 @default.
- W2003895100 hasConceptScore W2003895100C86803240 @default.
- W2003895100 hasIssue "1-4" @default.
- W2003895100 hasLocation W20038951001 @default.
- W2003895100 hasOpenAccess W2003895100 @default.
- W2003895100 hasPrimaryLocation W20038951001 @default.
- W2003895100 hasRelatedWork W1608877801 @default.
- W2003895100 hasRelatedWork W178953800 @default.
- W2003895100 hasRelatedWork W2049296849 @default.
- W2003895100 hasRelatedWork W2058774840 @default.
- W2003895100 hasRelatedWork W2076214836 @default.
- W2003895100 hasRelatedWork W2080782286 @default.
- W2003895100 hasRelatedWork W2403822735 @default.
- W2003895100 hasRelatedWork W2419246932 @default.
- W2003895100 hasRelatedWork W3107092011 @default.
- W2003895100 hasRelatedWork W390707090 @default.
- W2003895100 hasVolume "42" @default.
- W2003895100 isParatext "false" @default.
- W2003895100 isRetracted "false" @default.
- W2003895100 magId "2003895100" @default.
- W2003895100 workType "article" @default.