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• W2003971737 endingPage "6645" @default.
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• W2003971737 abstract "Nfu-type proteins are essential in the biogenesis of iron–sulfur (Fe-S) clusters in numerous organisms. A number of phenotypes including low levels of Fe-S cluster incorporation are associated with the deletion of the gene encoding a chloroplast-specific Nfu-type protein, Nfu2 from Arabidopsis thaliana (AtNfu2). Here, we report that recombinant AtNfu2 is able to assemble both [2Fe-2S] and [4Fe-4S] clusters. Analytical data and gel filtration studies support cluster/protein stoichiometries of one [2Fe-2S] cluster/homotetramer and one [4Fe-4S] cluster/homodimer. The combination of UV–visible absorption and circular dichroism and resonance Raman and Mössbauer spectroscopies has been employed to investigate the nature, properties, and transfer of the clusters assembled on Nfu2. The results are consistent with subunit-bridging [2Fe-2S]2+ and [4Fe-4S]2+ clusters coordinated by the cysteines in the conserved CXXC motif. The results also provided insight into the specificity of Nfu2 for the maturation of chloroplastic Fe-S proteins via intact, rapid, and quantitative cluster transfer. [2Fe-2S] cluster-bound Nfu2 is shown to be an effective [2Fe-2S]2+ cluster donor for glutaredoxin S16 but not glutaredoxin S14. Moreover, [4Fe-4S] cluster-bound Nfu2 is shown to be a very rapid and efficient [4Fe-4S]2+ cluster donor for adenosine 5′-phosphosulfate reductase (APR1), and yeast two-hybrid studies indicate that APR1 forms a complex with Nfu2 but not with Nfu1 and Nfu3, the two other chloroplastic Nfu proteins. This cluster transfer is likely to be physiologically relevant and is particularly significant for plant metabolism as APR1 catalyzes the second step in reductive sulfur assimilation, which ultimately results in the biosynthesis of cysteine, methionine, glutathione, and Fe-S clusters." @default.
• W2003971737 created "2016-06-24" @default.
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• W2003971737 date "2013-09-13" @default.
• W2003971737 modified "2023-10-13" @default.
• W2003971737 title "<i>Arabidopsis thaliana</i> Nfu2 Accommodates [2Fe-2S] or [4Fe-4S] Clusters and Is Competent for <i>in Vitro</i> Maturation of Chloroplast [2Fe-2S] and [4Fe-4S] Cluster-Containing Proteins" @default.
• W2003971737 cites W1538196126 @default.
• W2003971737 cites W1573546925 @default.
• W2003971737 cites W1754434570 @default.
• W2003971737 cites W1932705370 @default.
• W2003971737 cites W1966007187 @default.
• W2003971737 cites W1967781652 @default.
• W2003971737 cites W1972436650 @default.
• W2003971737 cites W1973422423 @default.
• W2003971737 cites W1974291399 @default.
• W2003971737 cites W1979073262 @default.
• W2003971737 cites W1984472162 @default.
• W2003971737 cites W1988272605 @default.
• W2003971737 cites W1995331522 @default.
• W2003971737 cites W1997352785 @default.
• W2003971737 cites W2002794726 @default.
• W2003971737 cites W2004845050 @default.
• W2003971737 cites W2010802040 @default.
• W2003971737 cites W2010961029 @default.
• W2003971737 cites W2017263653 @default.
• W2003971737 cites W2022479255 @default.
• W2003971737 cites W2028192694 @default.
• W2003971737 cites W2028892106 @default.
• W2003971737 cites W2028906413 @default.
• W2003971737 cites W2034410412 @default.
• W2003971737 cites W2035839314 @default.
• W2003971737 cites W2039621056 @default.
• W2003971737 cites W2039644207 @default.
• W2003971737 cites W2039859624 @default.
• W2003971737 cites W2044726248 @default.
• W2003971737 cites W2045327756 @default.
• W2003971737 cites W2052823571 @default.
• W2003971737 cites W2058033409 @default.
• W2003971737 cites W2059831206 @default.
• W2003971737 cites W2060053190 @default.
• W2003971737 cites W2061510311 @default.
• W2003971737 cites W2062424661 @default.
• W2003971737 cites W2062700139 @default.
• W2003971737 cites W2066510550 @default.
• W2003971737 cites W2067303294 @default.
• W2003971737 cites W2072207453 @default.
• W2003971737 cites W2073330688 @default.
• W2003971737 cites W2075888213 @default.
• W2003971737 cites W2077057034 @default.
• W2003971737 cites W2086660631 @default.
• W2003971737 cites W2088723535 @default.
• W2003971737 cites W2089518921 @default.
• W2003971737 cites W2090358554 @default.
• W2003971737 cites W2093241397 @default.
• W2003971737 cites W2095217602 @default.
• W2003971737 cites W2102071794 @default.
• W2003971737 cites W2105450030 @default.
• W2003971737 cites W2108465871 @default.
• W2003971737 cites W2114334676 @default.
• W2003971737 cites W2115498985 @default.
• W2003971737 cites W2116472408 @default.
• W2003971737 cites W2121450627 @default.
• W2003971737 cites W2122257408 @default.
• W2003971737 cites W2127645577 @default.
• W2003971737 cites W2139502531 @default.
• W2003971737 cites W2143644333 @default.
• W2003971737 cites W2153371171 @default.
• W2003971737 cites W2154307406 @default.
• W2003971737 cites W2161339684 @default.
• W2003971737 cites W2165626042 @default.
• W2003971737 cites W2168751548 @default.
• W2003971737 cites W2172226727 @default.
• W2003971737 cites W95436999 @default.
• W2003971737 doi "https://doi.org/10.1021/bi4007622" @default.
• W2003971737 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3819817" @default.
• W2003971737 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24032747" @default.
• W2003971737 hasPublicationYear "2013" @default.
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