FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2003973251> ?p ?o ?g. }

• W2003973251 endingPage "101" @default.
• W2003973251 startingPage "91" @default.
• W2003973251 abstract "The mycobacterial peptidoglycan has structure and biosynthetic pathways to similar those of other bacteria. UDP-N-acetylglucosamine enolpyruvyle transferase (MurA) catalyzes the first reaction in the biosynthesis of peptidoglycan. The MurA enzyme has been identified from various bacterial species, but the in-depth biochemical properties of mycobacterial MurA have not been characterized. In this study, both Mycobacterium tuberculosis MurA protein and Mycobacterium smegmatis MurA protein were overexpressed in Escherichia coli and purified by affinity chromatography. MurA activity was detected by HPLC. A colorimetric assay of MurA activity was also developed and the kinetic properties of Mtb MurA and Msm MurA were determined using this colorimetric assay. A conditional murA gene knockout strain was constructed by DNA homologous recombination. The disruption of murA in the genome of M. smegmatis led to loss of viability at a non-permissive temperature. Drastic morphological and structural alterations in the M. smegmatis murA knockout strain were observed by scanning electron microscopy and transmission electron microscopy. These results demonstrated that murA was an essential gene for growth of M. smegmatis. Therefore, MurA is a potential target for developing new anti-tuberculosis drugs." @default.
• W2003973251 created "2016-06-24" @default.
• W2003973251 creator A5003030781 @default.
• W2003973251 creator A5003554962 @default.
• W2003973251 creator A5013973657 @default.
• W2003973251 creator A5044754372 @default.
• W2003973251 creator A5045671519 @default.
• W2003973251 creator A5055320676 @default.
• W2003973251 creator A5065598450 @default.
• W2003973251 creator A5077245707 @default.
• W2003973251 creator A5087815553 @default.
• W2003973251 creator A5090166940 @default.
• W2003973251 date "2014-02-01" @default.
• W2003973251 modified "2023-10-09" @default.
• W2003973251 title "Characterization of mycobacterial UDP-N-acetylglucosamine enolpyruvyle transferase (MurA)" @default.
• W2003973251 cites W1971087365 @default.
• W2003973251 cites W1971961760 @default.
• W2003973251 cites W1979089302 @default.
• W2003973251 cites W1979381822 @default.
• W2003973251 cites W1993846180 @default.
• W2003973251 cites W1995405638 @default.
• W2003973251 cites W2010148230 @default.
• W2003973251 cites W2021593640 @default.
• W2003973251 cites W2036448942 @default.
• W2003973251 cites W2045538822 @default.
• W2003973251 cites W2046139772 @default.
• W2003973251 cites W2051135826 @default.
• W2003973251 cites W2054549410 @default.
• W2003973251 cites W2054859474 @default.
• W2003973251 cites W2065786511 @default.
• W2003973251 cites W2076952762 @default.
• W2003973251 cites W2094344799 @default.
• W2003973251 cites W2100735468 @default.
• W2003973251 cites W2104868544 @default.
• W2003973251 cites W2115499017 @default.
• W2003973251 cites W2128482141 @default.
• W2003973251 cites W2135415653 @default.
• W2003973251 cites W2141099568 @default.
• W2003973251 cites W2147680301 @default.
• W2003973251 cites W2148325233 @default.
• W2003973251 cites W2155179886 @default.
• W2003973251 cites W2272837046 @default.
• W2003973251 cites W4211162439 @default.
• W2003973251 doi "https://doi.org/10.1016/j.resmic.2014.01.004" @default.
• W2003973251 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24463011" @default.
• W2003973251 hasPublicationYear "2014" @default.
• W2003973251 type Work @default.
• W2003973251 sameAs 2003973251 @default.
• W2003973251 citedByCount "16" @default.
• W2003973251 countsByYear W20039732512016 @default.
• W2003973251 countsByYear W20039732512017 @default.
• W2003973251 countsByYear W20039732512019 @default.
• W2003973251 countsByYear W20039732512020 @default.
• W2003973251 countsByYear W20039732512021 @default.
• W2003973251 countsByYear W20039732512022 @default.
• W2003973251 crossrefType "journal-article" @default.
• W2003973251 hasAuthorship W2003973251A5003030781 @default.
• W2003973251 hasAuthorship W2003973251A5003554962 @default.
• W2003973251 hasAuthorship W2003973251A5013973657 @default.
• W2003973251 hasAuthorship W2003973251A5044754372 @default.
• W2003973251 hasAuthorship W2003973251A5045671519 @default.
• W2003973251 hasAuthorship W2003973251A5055320676 @default.
• W2003973251 hasAuthorship W2003973251A5065598450 @default.
• W2003973251 hasAuthorship W2003973251A5077245707 @default.
• W2003973251 hasAuthorship W2003973251A5087815553 @default.
• W2003973251 hasAuthorship W2003973251A5090166940 @default.
• W2003973251 hasConcept C104317684 @default.
• W2003973251 hasConcept C111919701 @default.
• W2003973251 hasConcept C128019096 @default.
• W2003973251 hasConcept C142724271 @default.
• W2003973251 hasConcept C181199279 @default.
• W2003973251 hasConcept C2776147852 @default.
• W2003973251 hasConcept C2776376580 @default.
• W2003973251 hasConcept C2776634448 @default.
• W2003973251 hasConcept C2777204207 @default.
• W2003973251 hasConcept C2777975735 @default.
• W2003973251 hasConcept C2781069245 @default.
• W2003973251 hasConcept C41008148 @default.
• W2003973251 hasConcept C523546767 @default.
• W2003973251 hasConcept C54355233 @default.
• W2003973251 hasConcept C547475151 @default.
• W2003973251 hasConcept C55493867 @default.
• W2003973251 hasConcept C71924100 @default.
• W2003973251 hasConcept C86803240 @default.
• W2003973251 hasConcept C89423630 @default.
• W2003973251 hasConceptScore W2003973251C104317684 @default.
• W2003973251 hasConceptScore W2003973251C111919701 @default.
• W2003973251 hasConceptScore W2003973251C128019096 @default.
• W2003973251 hasConceptScore W2003973251C142724271 @default.
• W2003973251 hasConceptScore W2003973251C181199279 @default.
• W2003973251 hasConceptScore W2003973251C2776147852 @default.
• W2003973251 hasConceptScore W2003973251C2776376580 @default.
• W2003973251 hasConceptScore W2003973251C2776634448 @default.
• W2003973251 hasConceptScore W2003973251C2777204207 @default.
• W2003973251 hasConceptScore W2003973251C2777975735 @default.
• W2003973251 hasConceptScore W2003973251C2781069245 @default.
• W2003973251 hasConceptScore W2003973251C41008148 @default.
• W2003973251 hasConceptScore W2003973251C523546767 @default.

• next