Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004010022> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2004010022 endingPage "363" @default.
- W2004010022 startingPage "358" @default.
- W2004010022 abstract "Preparative vertical and rotating horizontal (Rotofor) ampholine column and immobiline flat bed polyacrylamide gel isoelectric focusing were evaluated for the isolation of the biologically active acidic form of leukoregulin, a 50,000-Da glycoprotein lymphokine with tumor growth inhibitory activity. Leukoregulin secreted by normal human lymphocytes was concentrated by 10,000 nominal molecular weight size exclusion ultrafiltration and by DEAE anion exchange chromatography using step elution with 0.02 M Tris-HCl: 0.1 M NaCl, pH 7.4. Preparative isoelectric focusing was carried out in a 110-ml vertical column containing 1% ampholines in a pH 4-6 gradient at 15 W constant power for 16-18 h, in a Rotofor 55-ml horizontal column containing 2% ampholines in a pH 4-6 gradient at 12 W constant power for 4-6 h, or in an immobiline pH 4.5-6.5 gradient within a 5% polyacrylamide 120 X 110 X 5-mm flat bed gel at 3 W constant power for 16-18 h. Recovery of biologically active leukoregulin from the vertical and horizontal ampholine columns was similar. The pH 4.9-5.2 fractions from the Rotofor ampholine column contained 4-7% and the fractions from the immobiline gel contained 4% of the leukoregulin activity applied to the electrofocusing column or gel, respectively. Analytical immobiline isoelectric focusing of the leukoregulin in the pH 4.9-5.2 fractions from the Rotofor column demonstrated that a single silver staining band with a pI of 5.1 can be obtained by this rapid method of preparative isoelectric focusing." @default.
- W2004010022 created "2016-06-24" @default.
- W2004010022 creator A5004580992 @default.
- W2004010022 creator A5024285346 @default.
- W2004010022 creator A5070846532 @default.
- W2004010022 date "1989-03-01" @default.
- W2004010022 modified "2023-09-27" @default.
- W2004010022 title "Preparative isoelectric focusing in ampholine electrofocusing columns versus immobiline polyacrylamide gel for the purification of biologically active leukoregulin" @default.
- W2004010022 cites W1545196380 @default.
- W2004010022 cites W1545524757 @default.
- W2004010022 cites W1564327094 @default.
- W2004010022 cites W1569253030 @default.
- W2004010022 cites W1575089642 @default.
- W2004010022 cites W1965622703 @default.
- W2004010022 cites W1966535795 @default.
- W2004010022 cites W1971809107 @default.
- W2004010022 cites W1988556197 @default.
- W2004010022 cites W1988867096 @default.
- W2004010022 cites W1989871045 @default.
- W2004010022 cites W1990856566 @default.
- W2004010022 cites W1995096576 @default.
- W2004010022 cites W2014321075 @default.
- W2004010022 cites W2021605201 @default.
- W2004010022 cites W2045251316 @default.
- W2004010022 cites W2054465972 @default.
- W2004010022 cites W2069152461 @default.
- W2004010022 cites W2071580756 @default.
- W2004010022 cites W2071979729 @default.
- W2004010022 cites W2073679951 @default.
- W2004010022 cites W2075188972 @default.
- W2004010022 cites W2075480136 @default.
- W2004010022 cites W2082551783 @default.
- W2004010022 cites W2087025864 @default.
- W2004010022 cites W2094593070 @default.
- W2004010022 cites W2285166667 @default.
- W2004010022 cites W2394715609 @default.
- W2004010022 doi "https://doi.org/10.1016/0003-2697(89)90066-3" @default.
- W2004010022 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2729555" @default.
- W2004010022 hasPublicationYear "1989" @default.
- W2004010022 type Work @default.
- W2004010022 sameAs 2004010022 @default.
- W2004010022 citedByCount "24" @default.
- W2004010022 crossrefType "journal-article" @default.
- W2004010022 hasAuthorship W2004010022A5004580992 @default.
- W2004010022 hasAuthorship W2004010022A5024285346 @default.
- W2004010022 hasAuthorship W2004010022A5070846532 @default.
- W2004010022 hasConcept C101660505 @default.
- W2004010022 hasConcept C113196181 @default.
- W2004010022 hasConcept C181199279 @default.
- W2004010022 hasConcept C185592680 @default.
- W2004010022 hasConcept C188027245 @default.
- W2004010022 hasConcept C2778862431 @default.
- W2004010022 hasConcept C2781014771 @default.
- W2004010022 hasConcept C43617362 @default.
- W2004010022 hasConcept C50476208 @default.
- W2004010022 hasConcept C55493867 @default.
- W2004010022 hasConcept C73826308 @default.
- W2004010022 hasConcept C96294017 @default.
- W2004010022 hasConceptScore W2004010022C101660505 @default.
- W2004010022 hasConceptScore W2004010022C113196181 @default.
- W2004010022 hasConceptScore W2004010022C181199279 @default.
- W2004010022 hasConceptScore W2004010022C185592680 @default.
- W2004010022 hasConceptScore W2004010022C188027245 @default.
- W2004010022 hasConceptScore W2004010022C2778862431 @default.
- W2004010022 hasConceptScore W2004010022C2781014771 @default.
- W2004010022 hasConceptScore W2004010022C43617362 @default.
- W2004010022 hasConceptScore W2004010022C50476208 @default.
- W2004010022 hasConceptScore W2004010022C55493867 @default.
- W2004010022 hasConceptScore W2004010022C73826308 @default.
- W2004010022 hasConceptScore W2004010022C96294017 @default.
- W2004010022 hasIssue "2" @default.
- W2004010022 hasLocation W20040100221 @default.
- W2004010022 hasLocation W20040100222 @default.
- W2004010022 hasOpenAccess W2004010022 @default.
- W2004010022 hasPrimaryLocation W20040100221 @default.
- W2004010022 hasRelatedWork W1968784648 @default.
- W2004010022 hasRelatedWork W1978348000 @default.
- W2004010022 hasRelatedWork W1999145803 @default.
- W2004010022 hasRelatedWork W2024722039 @default.
- W2004010022 hasRelatedWork W2067754488 @default.
- W2004010022 hasRelatedWork W2091196785 @default.
- W2004010022 hasRelatedWork W2165515068 @default.
- W2004010022 hasRelatedWork W2321099364 @default.
- W2004010022 hasRelatedWork W2398624879 @default.
- W2004010022 hasRelatedWork W47688629 @default.
- W2004010022 hasVolume "177" @default.
- W2004010022 isParatext "false" @default.
- W2004010022 isRetracted "false" @default.
- W2004010022 magId "2004010022" @default.
- W2004010022 workType "article" @default.