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• W2004013965 abstract "Abstract Protein engineering is a powerful tool, which correlates protein structure with specific functions, both in applied biotechnology and in basic research. Here, we present a practical teaching course for engineering the green fluorescent protein (GFP) from Aequorea victoria by a random mutagenesis strategy using error‐prone polymerase chain reaction. Screening of bacterial colonies transformed with random mutant libraries identified GFP variants with increased fluorescence yields. Mapping the three‐dimensional structure of these mutants demonstrated how alterations in structural features such as the environment around the fluorophore and properties of the protein surface can influence functional properties such as the intensity of fluorescence and protein solubility. Biochemistry and Molecular Biology Education Vol. 39, No. 1, pp. 21‐27, 2011" @default.
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• W2004013965 date "2011-01-01" @default.
• W2004013965 modified "2023-10-13" @default.
• W2004013965 title "A practical teaching course in directed protein evolution using the green fluorescent protein as a model" @default.
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• W2004013965 doi "https://doi.org/10.1002/bmb.20430" @default.
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