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- W2004018000 abstract "Mesenchymal stem cells (MSCs) are adult stem cells which have the ability to differentiate into cells of the mesenchymal lineage, such as osteocyte, and other types of cells, such as hepatocyte-like cells when appropriated condition is applied. This makes them promising cell sources for bone and liver repair, and tracking the fate and function of transplanted MSCs in vivo become paramount in order to develop a novel therapeutic strategy in tissue repair. Quantum dots (QDs) are colloidal semiconductor nanocrystals which show a broad absorption band with a large and flexible cross-section allowing multiphoton microscopy. They also have a size tunable emission and high resistance to photobleaching. In addition, quantum dots provide an active surface for chemical conjugation with proteins, antibodies and short peptides. In this work Cadmium Telluride/Cadmium Sulfide (CdTe/CdS) quantum dots functionalized with mercaptosuccinic acid (MSA) was synthesized in aqueous medium and characterized by optical spectroscopy and X-Ray diffractometry. To increase cellular uptake and decrease nanoparticle toxicity, the QDs were then encapsulated into neutral and cationic liposomes (Lipo-QDs) and characterized by zeta potential (−20mV and +44 mV for neutral and cationic liposomes respectively),and transmission electron microscopy. The Lipo-QDs systems were incubated with MSCs and cellular uptake was measured by flow cytometry. An increase in labeling efficiency with positively charged liposome was observed when compared with the QDs alone and neutral liposomes. Lipo-QDs toxicities are under investigation. These preliminary data indicate that Lipo-QDs might be suitable for real time tracking of stem cells after transplantation, and thus might be a promising tool for imaging stem cell therapy in vivo." @default.
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- W2004018000 date "2014-01-01" @default.
- W2004018000 modified "2023-09-28" @default.
- W2004018000 title "Studies on Intracellular Delivery of Thiol-Capped Cdte Nanocrystals Mediated by Liposomes in Mesenchymal Stem Cells" @default.
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- W2004018000 doi "https://doi.org/10.1016/j.bpj.2013.11.3428" @default.
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