Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004087767> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2004087767 endingPage "218" @default.
- W2004087767 startingPage "209" @default.
- W2004087767 abstract "Hexamethylphosphoramide (HMPA) is an aprotic polar solvent and nasal carcinogen in rats. The metabolism of HMPA to formaldehyde, another nasal carcinogen in rats, was found to be approximately 6 times greater in microsomes from olfactory tissues than from respiratory tissues (isolated from both male and female rats). HMPA was shown to induce formation of DNA-protein crosslinks (DPXLS) in isolated rat nasal epithelial cells. Using a filter binding assay, we demonstrated that microsomal activation is necessary for HMPA-induced crosslink formation between plasmid DNA and calf thymus histones, presumably through metabolic N-demethylation of HMPA and the formation of formaldehyde. Both formaldehyde production and DPXL formation were inhibited by pre-incubation of nasal mucosal extracts with metyrapone, an inhibitor of cytochrome P-450. Significant dose-dependent increases in DPXL formation were observed in respiratory and olfactory epithelial cells exposed to > or = 0.5 and 1 mM HMPA, respectively, for 3 h at 37 degrees C. This resulted in DPXL accumulation at 18-20% higher levels than untreated cells. Increases in DPXL formation in rat nasal epithelial cells cultured with 1 mM HMPA were inhibited by over 70% by co-administration of metyrapone. These data suggest that metabolic liberation of formaldehyde from HMPA is involved in the mechanism of HMPA-induced nasal carcinogenesis. Comparative studies showed formaldehyde to be more potent than HMPA in the induction of DPXL in nasal epithelium. However, induction of tumor formation after two years at 50 ppb HMPA and 6 ppm formaldehyde show the former to be active at several-fold lower concentrations. Therefore, other mechanisms are likely to be involved in HMPA nasal carcinogenesis." @default.
- W2004087767 created "2016-06-24" @default.
- W2004087767 creator A5003574675 @default.
- W2004087767 creator A5006678476 @default.
- W2004087767 creator A5059289128 @default.
- W2004087767 date "1995-07-01" @default.
- W2004087767 modified "2023-10-16" @default.
- W2004087767 title "DNA-protein crosslink formation in rat nasal epithelial cells by hexamethylphosphoramide and its correlation with formaldehyde production" @default.
- W2004087767 cites W159023385 @default.
- W2004087767 cites W1972534126 @default.
- W2004087767 cites W1977779461 @default.
- W2004087767 cites W2004618089 @default.
- W2004087767 cites W2028451444 @default.
- W2004087767 cites W2033974841 @default.
- W2004087767 cites W2056810217 @default.
- W2004087767 cites W2056912401 @default.
- W2004087767 cites W2057908979 @default.
- W2004087767 cites W2057915537 @default.
- W2004087767 cites W2058170465 @default.
- W2004087767 cites W2068261356 @default.
- W2004087767 cites W2081051994 @default.
- W2004087767 cites W2084356323 @default.
- W2004087767 cites W2095311817 @default.
- W2004087767 cites W2099481136 @default.
- W2004087767 cites W2134200987 @default.
- W2004087767 doi "https://doi.org/10.1016/0165-1218(95)90016-0" @default.
- W2004087767 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7623875" @default.
- W2004087767 hasPublicationYear "1995" @default.
- W2004087767 type Work @default.
- W2004087767 sameAs 2004087767 @default.
- W2004087767 citedByCount "21" @default.
- W2004087767 countsByYear W20040877672012 @default.
- W2004087767 countsByYear W20040877672013 @default.
- W2004087767 countsByYear W20040877672014 @default.
- W2004087767 countsByYear W20040877672015 @default.
- W2004087767 crossrefType "journal-article" @default.
- W2004087767 hasAuthorship W2004087767A5003574675 @default.
- W2004087767 hasAuthorship W2004087767A5006678476 @default.
- W2004087767 hasAuthorship W2004087767A5059289128 @default.
- W2004087767 hasConcept C114246631 @default.
- W2004087767 hasConcept C126322002 @default.
- W2004087767 hasConcept C134018914 @default.
- W2004087767 hasConcept C153911025 @default.
- W2004087767 hasConcept C155647269 @default.
- W2004087767 hasConcept C170493617 @default.
- W2004087767 hasConcept C185592680 @default.
- W2004087767 hasConcept C2775930285 @default.
- W2004087767 hasConcept C2778006127 @default.
- W2004087767 hasConcept C2780187430 @default.
- W2004087767 hasConcept C55493867 @default.
- W2004087767 hasConcept C71924100 @default.
- W2004087767 hasConcept C86803240 @default.
- W2004087767 hasConceptScore W2004087767C114246631 @default.
- W2004087767 hasConceptScore W2004087767C126322002 @default.
- W2004087767 hasConceptScore W2004087767C134018914 @default.
- W2004087767 hasConceptScore W2004087767C153911025 @default.
- W2004087767 hasConceptScore W2004087767C155647269 @default.
- W2004087767 hasConceptScore W2004087767C170493617 @default.
- W2004087767 hasConceptScore W2004087767C185592680 @default.
- W2004087767 hasConceptScore W2004087767C2775930285 @default.
- W2004087767 hasConceptScore W2004087767C2778006127 @default.
- W2004087767 hasConceptScore W2004087767C2780187430 @default.
- W2004087767 hasConceptScore W2004087767C55493867 @default.
- W2004087767 hasConceptScore W2004087767C71924100 @default.
- W2004087767 hasConceptScore W2004087767C86803240 @default.
- W2004087767 hasIssue "4" @default.
- W2004087767 hasLocation W20040877671 @default.
- W2004087767 hasLocation W20040877672 @default.
- W2004087767 hasOpenAccess W2004087767 @default.
- W2004087767 hasPrimaryLocation W20040877671 @default.
- W2004087767 hasRelatedWork W143882305 @default.
- W2004087767 hasRelatedWork W1540767738 @default.
- W2004087767 hasRelatedWork W1983975256 @default.
- W2004087767 hasRelatedWork W1993962850 @default.
- W2004087767 hasRelatedWork W2045783720 @default.
- W2004087767 hasRelatedWork W2047142873 @default.
- W2004087767 hasRelatedWork W2080005266 @default.
- W2004087767 hasRelatedWork W2088609120 @default.
- W2004087767 hasRelatedWork W2468568572 @default.
- W2004087767 hasRelatedWork W2741992356 @default.
- W2004087767 hasVolume "343" @default.
- W2004087767 isParatext "false" @default.
- W2004087767 isRetracted "false" @default.
- W2004087767 magId "2004087767" @default.
- W2004087767 workType "article" @default.