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• W2004135280 startingPage "968" @default.
• W2004135280 abstract "Given the HIV epidemic in Malaysia, genetic information on opportunistic pathogens, such as Cryptosporidium and Giardia, in HIV/AIDS patients is pivotal to enhance our understanding of epidemiology, patient care, management and disease surveillance. In the present study, 122 faecal samples from HIV/AIDS patients were examined for the presence of Cryptosporidium oocysts and Giardia cysts using a conventional coproscopic approach. Such oocysts and cysts were detected in 22.1% and 5.7% of the 122 faecal samples, respectively. Genomic DNAs from selected samples were tested in a nested-PCR, targeting regions of the small subunit (SSU) of nuclear ribosomal RNA and the 60 kDa glycoprotein (gp60) genes (for Cryptosporidium), and the triose-phosphate isomerase (tpi) gene (for Giardia), followed by direct sequencing. The sequencing of amplicons derived from SSU revealed that Cryptosporidium parvum was the most frequently detected species (64% of 25 samples tested), followed by C. hominis (24%), C. meleagridis (8%) and C. felis (4%). Sequencing of a region of gp60 identified C. parvum subgenotype IIdA15G2R1 and C. hominis subgenotypes IaA14R1, IbA10G2R2, IdA15R2, IeA11G2T3R1 and IfA11G1R2. Sequencing of amplicons derived from tpi revealed G. duodenalis assemblage A, which is of zoonotic importance. This is the first report of C. hominis, C. meleagridis and C. felis from Malaysian HIV/AIDS patients. Future work should focus on an extensive analysis of Cryptosporidium and Giardia in such patients as well as in domestic and wild animals, in order to improve the understanding of transmission patterns and dynamics in Malaysia. It would also be particularly interesting to establish the relationship among clinical manifestation, CD4 cell counts and genotypes/subgenotypes of Cryptosporidium and Giardia in HIV/AIDS patients. Such insights would assist in a better management of clinical disease in immuno-deficient patients as well as improved preventive and control strategies." @default.
• W2004135280 created "2016-06-24" @default.
• W2004135280 creator A5013781301 @default.
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• W2004135280 creator A5085039675 @default.
• W2004135280 date "2011-07-01" @default.
• W2004135280 modified "2023-09-23" @default.
• W2004135280 title "First genetic classification of Cryptosporidium and Giardia from HIV/AIDS patients in Malaysia" @default.
• W2004135280 cites W1527595659 @default.
• W2004135280 cites W152975134 @default.
• W2004135280 cites W1586826587 @default.
• W2004135280 cites W1882107025 @default.
• W2004135280 cites W1963712124 @default.
• W2004135280 cites W1964729655 @default.
• W2004135280 cites W1970083470 @default.
• W2004135280 cites W1970620530 @default.
• W2004135280 cites W1976467906 @default.
• W2004135280 cites W1978083480 @default.
• W2004135280 cites W1987635298 @default.
• W2004135280 cites W1992516181 @default.
• W2004135280 cites W1993192822 @default.
• W2004135280 cites W1994670400 @default.
• W2004135280 cites W1996373671 @default.
• W2004135280 cites W1997100943 @default.
• W2004135280 cites W1997391434 @default.
• W2004135280 cites W2000605251 @default.
• W2004135280 cites W2000903795 @default.
• W2004135280 cites W2001374390 @default.
• W2004135280 cites W2005595540 @default.
• W2004135280 cites W2013561307 @default.
• W2004135280 cites W2017803930 @default.
• W2004135280 cites W2019858741 @default.
• W2004135280 cites W2024128949 @default.
• W2004135280 cites W2024988556 @default.
• W2004135280 cites W2030684171 @default.
• W2004135280 cites W2033179250 @default.
• W2004135280 cites W2035931483 @default.
• W2004135280 cites W2036244972 @default.
• W2004135280 cites W2038481023 @default.
• W2004135280 cites W2039657744 @default.
• W2004135280 cites W2039784294 @default.
• W2004135280 cites W2040560178 @default.
• W2004135280 cites W2044224590 @default.
• W2004135280 cites W2046870643 @default.
• W2004135280 cites W2047084551 @default.
• W2004135280 cites W2047194251 @default.
• W2004135280 cites W2049074801 @default.
• W2004135280 cites W2049123601 @default.
• W2004135280 cites W2059519883 @default.
• W2004135280 cites W2068828166 @default.
• W2004135280 cites W2071615747 @default.
• W2004135280 cites W2075683777 @default.
• W2004135280 cites W2081249972 @default.
• W2004135280 cites W2081292646 @default.
• W2004135280 cites W2081919191 @default.
• W2004135280 cites W2082120087 @default.
• W2004135280 cites W2082928585 @default.
• W2004135280 cites W2085737527 @default.
• W2004135280 cites W2086754376 @default.
• W2004135280 cites W2091028119 @default.
• W2004135280 cites W2092482171 @default.
• W2004135280 cites W2096242209 @default.
• W2004135280 cites W2097382368 @default.
• W2004135280 cites W2098549617 @default.
• W2004135280 cites W2101083369 @default.
• W2004135280 cites W2101942134 @default.
• W2004135280 cites W2109164662 @default.
• W2004135280 cites W2110947778 @default.
• W2004135280 cites W2112812941 @default.
• W2004135280 cites W2113443519 @default.
• W2004135280 cites W2113475228 @default.
• W2004135280 cites W2115182954 @default.
• W2004135280 cites W2116477129 @default.
• W2004135280 cites W2116958065 @default.
• W2004135280 cites W2118049781 @default.
• W2004135280 cites W2123216442 @default.
• W2004135280 cites W2124706578 @default.
• W2004135280 cites W2127777301 @default.
• W2004135280 cites W2133431467 @default.
• W2004135280 cites W2135163002 @default.
• W2004135280 cites W2136090235 @default.
• W2004135280 cites W2137476535 @default.
• W2004135280 cites W2140370872 @default.
• W2004135280 cites W2144647173 @default.
• W2004135280 cites W2144688025 @default.
• W2004135280 cites W2146058063 @default.
• W2004135280 cites W2148573794 @default.
• W2004135280 cites W2153916165 @default.
• W2004135280 cites W2154321650 @default.
• W2004135280 cites W2155761019 @default.
• W2004135280 cites W2156203983 @default.
• W2004135280 cites W2158892878 @default.
• W2004135280 cites W2160778184 @default.

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