FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004136340> ?p ?o ?g. }

• W2004136340 endingPage "2820" @default.
• W2004136340 startingPage "2811" @default.
• W2004136340 abstract "A series of equilibrium and kinetic measurements involving the oxidation-reduction properties of purified rat liver NADPH-cytochrome P-450 reductase and eight different purified rat liver cytochromes P-450 (P-450s) were carried out. Apparent spin states of P-450 iron were determined in the absence and presence of a number of known substrates by using second-derivative and conventional near-UV absorbance spectroscopy. Many of the substrates examined did not produce significant changes in the apparent iron spin state, even when binding could be demonstrated with equilibrium dialysis. Further, the spin state was not correlated to catalytic activity of the P-450s in reconstituted enzyme systems. The oxidation-reduction potentials were determined for the ferric/ferrous couples of each of the eight P-450s in the presence and absence of known substrates, as well as other proteins suspected of altering the potentials. The midpoint potential (Em,7) ranged from -350 to -289 mV for the P-450s under these conditions. In some cases Em,7 was raised with the addition of substrates, but the extent of the increase was no greater than +33 mV. The Em,7 of one P-450 (P-450 beta NF/ISF-G) was not changed significantly when the fraction of high-spin iron varied between 11 and 67%. Steady-state spectral studies provided evidence for the accumulation of an oxygenated ferrous intermediate (or a derived product) of one P-450 (P-450PB-B) in the presence of a substrate, cyclohexane. Studies on the donation of electrons from cytochrome b5 and a series of dyes to this complex suggest that it has an effective Em,7 (for reduction) of approximately +50 mV. In studies with one of the P-450s, steady-state spectral studies indicated that the three-electron-reduced form of NADPH-P-450 reductase accumulates, consistent with the view that this form of the reductase is involved in the reduction of P-450 from the ferric to the ferrous state." @default.
• W2004136340 created "2016-06-24" @default.
• W2004136340 creator A5025977173 @default.
• W2004136340 date "1983-06-07" @default.
• W2004136340 modified "2023-09-27" @default.
• W2004136340 title "Oxidation-reduction properties of rat liver cytochromes P-450 and NADPH-cytochrome P-450 reductase related to catalysis in reconstituted systems" @default.
• W2004136340 cites W105949446 @default.
• W2004136340 cites W136355807 @default.
• W2004136340 cites W1488421511 @default.
• W2004136340 cites W1503960664 @default.
• W2004136340 cites W1504594972 @default.
• W2004136340 cites W1554826669 @default.
• W2004136340 cites W1595165457 @default.
• W2004136340 cites W1597669330 @default.
• W2004136340 cites W1603055893 @default.
• W2004136340 cites W1617709375 @default.
• W2004136340 cites W1676611497 @default.
• W2004136340 cites W1781030166 @default.
• W2004136340 cites W1828405647 @default.
• W2004136340 cites W1845627550 @default.
• W2004136340 cites W1946732322 @default.
• W2004136340 cites W1984449938 @default.
• W2004136340 cites W1988617504 @default.
• W2004136340 cites W1992413871 @default.
• W2004136340 cites W1996513112 @default.
• W2004136340 cites W2000983010 @default.
• W2004136340 cites W2008689037 @default.
• W2004136340 cites W2011019224 @default.
• W2004136340 cites W2011689199 @default.
• W2004136340 cites W2022628424 @default.
• W2004136340 cites W2029986319 @default.
• W2004136340 cites W2034242966 @default.
• W2004136340 cites W2041096346 @default.
• W2004136340 cites W2052987448 @default.
• W2004136340 cites W2058691531 @default.
• W2004136340 cites W2062254955 @default.
• W2004136340 cites W2070762715 @default.
• W2004136340 cites W2074352077 @default.
• W2004136340 cites W2092061087 @default.
• W2004136340 cites W2095285273 @default.
• W2004136340 cites W2169838870 @default.
• W2004136340 cites W2992741864 @default.
• W2004136340 cites W70981372 @default.
• W2004136340 cites W852648 @default.
• W2004136340 doi "https://doi.org/10.1021/bi00281a007" @default.
• W2004136340 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6307349" @default.
• W2004136340 hasPublicationYear "1983" @default.
• W2004136340 type Work @default.
• W2004136340 sameAs 2004136340 @default.
• W2004136340 citedByCount "170" @default.
• W2004136340 countsByYear W20041363402012 @default.
• W2004136340 countsByYear W20041363402013 @default.
• W2004136340 countsByYear W20041363402014 @default.
• W2004136340 countsByYear W20041363402015 @default.
• W2004136340 countsByYear W20041363402016 @default.
• W2004136340 countsByYear W20041363402017 @default.
• W2004136340 countsByYear W20041363402018 @default.
• W2004136340 countsByYear W20041363402019 @default.
• W2004136340 countsByYear W20041363402020 @default.
• W2004136340 countsByYear W20041363402021 @default.
• W2004136340 countsByYear W20041363402022 @default.
• W2004136340 countsByYear W20041363402023 @default.
• W2004136340 crossrefType "journal-article" @default.
• W2004136340 hasAuthorship W2004136340A5025977173 @default.
• W2004136340 hasConcept C134651460 @default.
• W2004136340 hasConcept C161790260 @default.
• W2004136340 hasConcept C178790620 @default.
• W2004136340 hasConcept C179104552 @default.
• W2004136340 hasConcept C181199279 @default.
• W2004136340 hasConcept C185592680 @default.
• W2004136340 hasConcept C18903297 @default.
• W2004136340 hasConcept C2775832776 @default.
• W2004136340 hasConcept C2777289219 @default.
• W2004136340 hasConcept C2780534640 @default.
• W2004136340 hasConcept C2780768313 @default.
• W2004136340 hasConcept C55493867 @default.
• W2004136340 hasConcept C71240020 @default.
• W2004136340 hasConcept C86803240 @default.
• W2004136340 hasConceptScore W2004136340C134651460 @default.
• W2004136340 hasConceptScore W2004136340C161790260 @default.
• W2004136340 hasConceptScore W2004136340C178790620 @default.
• W2004136340 hasConceptScore W2004136340C179104552 @default.
• W2004136340 hasConceptScore W2004136340C181199279 @default.
• W2004136340 hasConceptScore W2004136340C185592680 @default.
• W2004136340 hasConceptScore W2004136340C18903297 @default.
• W2004136340 hasConceptScore W2004136340C2775832776 @default.
• W2004136340 hasConceptScore W2004136340C2777289219 @default.
• W2004136340 hasConceptScore W2004136340C2780534640 @default.
• W2004136340 hasConceptScore W2004136340C2780768313 @default.
• W2004136340 hasConceptScore W2004136340C55493867 @default.
• W2004136340 hasConceptScore W2004136340C71240020 @default.
• W2004136340 hasConceptScore W2004136340C86803240 @default.
• W2004136340 hasIssue "12" @default.
• W2004136340 hasLocation W20041363401 @default.
• W2004136340 hasLocation W20041363402 @default.
• W2004136340 hasOpenAccess W2004136340 @default.
• W2004136340 hasPrimaryLocation W20041363401 @default.
• W2004136340 hasRelatedWork W1976038178 @default.

• next