Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004138468> ?p ?o ?g. }
- W2004138468 endingPage "837" @default.
- W2004138468 startingPage "831" @default.
- W2004138468 abstract "ABSTRACT Purification of xylulose 5-phosphate phosphoketolase (XpkA), the central enzyme of the phosphoketolase pathway (PKP) in lactic acid bacteria, and cloning and sequence analysis of the encoding gene, xpkA , from Lactobacillus pentosus MD363 are described. xpkA encodes a 788-amino-acid protein with a calculated mass of 88,705 Da. Expression of xpkA in Escherichia coli led to an increase in XpkA activity, while an xpkA knockout mutant of L. pentosus lost XpkA activity and was not able to grow on energy sources that are fermented via the PKP, indicating that xpkA encodes an enzyme with phosphoketolase activity. A database search revealed that there are high levels of similarity between XpkA and a phosphoketolase from Bifidobacterium lactis and between XpkA and a (putative) protein present in a number of evolutionarily distantly related organisms (up to 54% identical residues). Expression of xpkA in L. pentosus was induced by sugars that are fermented via the PKP and was repressed by glucose mediated by carbon catabolite protein A (CcpA) and by the mannose phosphoenolpyruvate phosphotransferase system. Most of the residues involved in correct binding of the cofactor thiamine pyrophosphate (TPP) that are conserved in transketolase, pyruvate decarboxylase, and pyruvate oxidase were also conserved at a similar position in XpkA, implying that there is a similar TPP-binding fold in XpkA." @default.
- W2004138468 created "2016-06-24" @default.
- W2004138468 creator A5044282246 @default.
- W2004138468 creator A5063650601 @default.
- W2004138468 creator A5064322566 @default.
- W2004138468 creator A5065308563 @default.
- W2004138468 creator A5083195785 @default.
- W2004138468 creator A5084626850 @default.
- W2004138468 date "2002-02-01" @default.
- W2004138468 modified "2023-09-29" @default.
- W2004138468 title "Expression of the Xylulose 5-Phosphate Phosphoketolase Gene, <i>xpkA</i> , from <i>Lactobacillus pentosus</i> MD363 Is Induced by Sugars That Are Fermented via the Phosphoketolase Pathway and Is Repressed by Glucose Mediated by CcpA and the Mannose Phosphoenolpyruvate Phosphotransferase System" @default.
- W2004138468 cites W118386176 @default.
- W2004138468 cites W1492117602 @default.
- W2004138468 cites W1899210214 @default.
- W2004138468 cites W1974943951 @default.
- W2004138468 cites W1996726147 @default.
- W2004138468 cites W2001992240 @default.
- W2004138468 cites W2017399258 @default.
- W2004138468 cites W2021230878 @default.
- W2004138468 cites W2026225179 @default.
- W2004138468 cites W2034371095 @default.
- W2004138468 cites W2039844563 @default.
- W2004138468 cites W2059594947 @default.
- W2004138468 cites W2081422634 @default.
- W2004138468 cites W2093655718 @default.
- W2004138468 cites W2104027427 @default.
- W2004138468 cites W2106070933 @default.
- W2004138468 cites W2123569104 @default.
- W2004138468 cites W2123812704 @default.
- W2004138468 cites W2139495241 @default.
- W2004138468 cites W2148572296 @default.
- W2004138468 cites W2250496884 @default.
- W2004138468 cites W33622858 @default.
- W2004138468 cites W4245947453 @default.
- W2004138468 doi "https://doi.org/10.1128/aem.68.2.831-837.2002" @default.
- W2004138468 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/126734" @default.
- W2004138468 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11823225" @default.
- W2004138468 hasPublicationYear "2002" @default.
- W2004138468 type Work @default.
- W2004138468 sameAs 2004138468 @default.
- W2004138468 citedByCount "53" @default.
- W2004138468 countsByYear W20041384682013 @default.
- W2004138468 countsByYear W20041384682014 @default.
- W2004138468 countsByYear W20041384682015 @default.
- W2004138468 countsByYear W20041384682017 @default.
- W2004138468 countsByYear W20041384682019 @default.
- W2004138468 countsByYear W20041384682020 @default.
- W2004138468 countsByYear W20041384682021 @default.
- W2004138468 countsByYear W20041384682023 @default.
- W2004138468 crossrefType "journal-article" @default.
- W2004138468 hasAuthorship W2004138468A5044282246 @default.
- W2004138468 hasAuthorship W2004138468A5063650601 @default.
- W2004138468 hasAuthorship W2004138468A5064322566 @default.
- W2004138468 hasAuthorship W2004138468A5065308563 @default.
- W2004138468 hasAuthorship W2004138468A5083195785 @default.
- W2004138468 hasAuthorship W2004138468A5084626850 @default.
- W2004138468 hasBestOaLocation W20041384682 @default.
- W2004138468 hasConcept C103432212 @default.
- W2004138468 hasConcept C104317684 @default.
- W2004138468 hasConcept C143065580 @default.
- W2004138468 hasConcept C181199279 @default.
- W2004138468 hasConcept C185592680 @default.
- W2004138468 hasConcept C197957613 @default.
- W2004138468 hasConcept C2777344606 @default.
- W2004138468 hasConcept C2780047943 @default.
- W2004138468 hasConcept C2781281423 @default.
- W2004138468 hasConcept C55493867 @default.
- W2004138468 hasConcept C83941689 @default.
- W2004138468 hasConcept C86803240 @default.
- W2004138468 hasConceptScore W2004138468C103432212 @default.
- W2004138468 hasConceptScore W2004138468C104317684 @default.
- W2004138468 hasConceptScore W2004138468C143065580 @default.
- W2004138468 hasConceptScore W2004138468C181199279 @default.
- W2004138468 hasConceptScore W2004138468C185592680 @default.
- W2004138468 hasConceptScore W2004138468C197957613 @default.
- W2004138468 hasConceptScore W2004138468C2777344606 @default.
- W2004138468 hasConceptScore W2004138468C2780047943 @default.
- W2004138468 hasConceptScore W2004138468C2781281423 @default.
- W2004138468 hasConceptScore W2004138468C55493867 @default.
- W2004138468 hasConceptScore W2004138468C83941689 @default.
- W2004138468 hasConceptScore W2004138468C86803240 @default.
- W2004138468 hasIssue "2" @default.
- W2004138468 hasLocation W20041384681 @default.
- W2004138468 hasLocation W20041384682 @default.
- W2004138468 hasLocation W20041384683 @default.
- W2004138468 hasLocation W20041384684 @default.
- W2004138468 hasOpenAccess W2004138468 @default.
- W2004138468 hasPrimaryLocation W20041384681 @default.
- W2004138468 hasRelatedWork W1985334975 @default.
- W2004138468 hasRelatedWork W1990846267 @default.
- W2004138468 hasRelatedWork W2008985653 @default.
- W2004138468 hasRelatedWork W2040106523 @default.
- W2004138468 hasRelatedWork W2065781312 @default.
- W2004138468 hasRelatedWork W2082729015 @default.
- W2004138468 hasRelatedWork W2095419453 @default.
- W2004138468 hasRelatedWork W2159559088 @default.
- W2004138468 hasRelatedWork W2208020336 @default.
- W2004138468 hasRelatedWork W4250471400 @default.