FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004139194> ?p ?o ?g. }

• W2004139194 endingPage "945" @default.
• W2004139194 startingPage "939" @default.
• W2004139194 abstract "Recently, lamivudine used to treat patients with hepatitis B virus (HBV) infection was revealed to have potent antiviral activity. However, HBV resistance to lamivudine has been reported and shown to have amino acid substitutions in the methionine residue of the conserved tyrosine (Y), methionine (M), aspartate (D), aspartate (D) motif of RNA-dependent DNA polymerase. To explore the consequences of substitutions in this motif (YMDD), we made 7 variants by substituting the methionine of the YMDD motif with isoleucine (I), valine (V), alanine (A), leucine (L), lysine (K), arginine (R), and threonine (T). Replication ability of these variants was evaluated by transfection into human hepatoma cells. Sensitivity to lamivudine was tested for replication-competent variants. Four variants with hydrophobic substitutions (I, V, A, and L) remained replication-competent, whereas 3 others with hydrophilic substitutions (K, R, and T) exhibited impaired replication. Of the 4 replication-competent variants, 2 (I and V) were resistant, and 2 (A and L) were sensitive to lamivudine. Because the polymerase and the surface gene overlap, the introduction of these mutations affected the secretion of hepatitis B surface antigen (HBsAg), namely 4 variants (I, V, L, and R) secreted HBsAg, whereas 3 variants (A, K, and T) did not. Our study elucidated that only one amino acid substitution in the YMDD motif was sufficient to cause lamivudine resistance in vitro. As a result of replication competence and lamivudine sensitivity, only viruses having YIDD or YVDD sequences may appear during treatment with lamivudine. This in vitro system could be used to study HBV mutations, replication competence, and their susceptibility to antivirals." @default.
• W2004139194 created "2016-06-24" @default.
• W2004139194 creator A5005637906 @default.
• W2004139194 creator A5017931660 @default.
• W2004139194 creator A5022316786 @default.
• W2004139194 creator A5034244372 @default.
• W2004139194 creator A5041139736 @default.
• W2004139194 creator A5067940252 @default.
• W2004139194 creator A5077269939 @default.
• W2004139194 date "1999-03-01" @default.
• W2004139194 modified "2023-10-17" @default.
• W2004139194 title "YMDD motif in hepatitis B virus DNA polymerase influences on replication and lamivudine resistance: A study byin vitrofull-length viral DNA transfection" @default.
• W2004139194 cites W1510860856 @default.
• W2004139194 cites W1525086512 @default.
• W2004139194 cites W1576999979 @default.
• W2004139194 cites W1582561043 @default.
• W2004139194 cites W1780433609 @default.
• W2004139194 cites W1857344328 @default.
• W2004139194 cites W1973313228 @default.
• W2004139194 cites W1973621959 @default.
• W2004139194 cites W1984681781 @default.
• W2004139194 cites W2004561527 @default.
• W2004139194 cites W2004705863 @default.
• W2004139194 cites W2007423548 @default.
• W2004139194 cites W2009581957 @default.
• W2004139194 cites W2017854107 @default.
• W2004139194 cites W2042653073 @default.
• W2004139194 cites W2044524906 @default.
• W2004139194 cites W2044553804 @default.
• W2004139194 cites W2052958019 @default.
• W2004139194 cites W2053135778 @default.
• W2004139194 cites W2055270948 @default.
• W2004139194 cites W2074433769 @default.
• W2004139194 cites W2084858048 @default.
• W2004139194 cites W2090476883 @default.
• W2004139194 cites W2101754090 @default.
• W2004139194 cites W2112150016 @default.
• W2004139194 cites W2129923672 @default.
• W2004139194 cites W2132542609 @default.
• W2004139194 cites W2141883500 @default.
• W2004139194 cites W2150082576 @default.
• W2004139194 cites W2151850780 @default.
• W2004139194 cites W2156552801 @default.
• W2004139194 cites W2166336747 @default.
• W2004139194 cites W2167572870 @default.
• W2004139194 cites W2318639814 @default.
• W2004139194 cites W2321075294 @default.
• W2004139194 cites W2323719395 @default.
• W2004139194 cites W4245723880 @default.
• W2004139194 doi "https://doi.org/10.1002/hep.510290340" @default.
• W2004139194 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10051501" @default.
• W2004139194 hasPublicationYear "1999" @default.
• W2004139194 type Work @default.
• W2004139194 sameAs 2004139194 @default.
• W2004139194 citedByCount "153" @default.
• W2004139194 countsByYear W20041391942012 @default.
• W2004139194 countsByYear W20041391942013 @default.
• W2004139194 countsByYear W20041391942014 @default.
• W2004139194 countsByYear W20041391942015 @default.
• W2004139194 countsByYear W20041391942016 @default.
• W2004139194 countsByYear W20041391942017 @default.
• W2004139194 countsByYear W20041391942018 @default.
• W2004139194 countsByYear W20041391942020 @default.
• W2004139194 countsByYear W20041391942021 @default.
• W2004139194 countsByYear W20041391942023 @default.
• W2004139194 crossrefType "journal-article" @default.
• W2004139194 hasAuthorship W2004139194A5005637906 @default.
• W2004139194 hasAuthorship W2004139194A5017931660 @default.
• W2004139194 hasAuthorship W2004139194A5022316786 @default.
• W2004139194 hasAuthorship W2004139194A5034244372 @default.
• W2004139194 hasAuthorship W2004139194A5041139736 @default.
• W2004139194 hasAuthorship W2004139194A5067940252 @default.
• W2004139194 hasAuthorship W2004139194A5077269939 @default.
• W2004139194 hasBestOaLocation W20041391941 @default.
• W2004139194 hasConcept C140704245 @default.
• W2004139194 hasConcept C153911025 @default.
• W2004139194 hasConcept C159047783 @default.
• W2004139194 hasConcept C2522874641 @default.
• W2004139194 hasConcept C2776580952 @default.
• W2004139194 hasConcept C2777305461 @default.
• W2004139194 hasConcept C2777410769 @default.
• W2004139194 hasConcept C2777869810 @default.
• W2004139194 hasConcept C2780593183 @default.
• W2004139194 hasConcept C2780912031 @default.
• W2004139194 hasConcept C515207424 @default.
• W2004139194 hasConcept C552990157 @default.
• W2004139194 hasConcept C55493867 @default.
• W2004139194 hasConcept C82381507 @default.
• W2004139194 hasConcept C86803240 @default.
• W2004139194 hasConceptScore W2004139194C140704245 @default.
• W2004139194 hasConceptScore W2004139194C153911025 @default.
• W2004139194 hasConceptScore W2004139194C159047783 @default.
• W2004139194 hasConceptScore W2004139194C2522874641 @default.
• W2004139194 hasConceptScore W2004139194C2776580952 @default.
• W2004139194 hasConceptScore W2004139194C2777305461 @default.
• W2004139194 hasConceptScore W2004139194C2777410769 @default.
• W2004139194 hasConceptScore W2004139194C2777869810 @default.
• W2004139194 hasConceptScore W2004139194C2780593183 @default.

• next