Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004149462> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2004149462 endingPage "207" @default.
- W2004149462 startingPage "199" @default.
- W2004149462 abstract "Transient expression of PEG mediated DNA uptake was monitored in protoplasts of carrot (Daucus carota L. cv. Apollo, Risø and Navona), rapeseed (Brassica napus L. cv. Line) and soybean (Glycine max L. cv. Mandarin) by measuring the GUS activity of a chimeric CaMV 35S-GUS reporter gene inserted in a 5.6-kb pBI 221.1 plasmid. Identity of the GUS enzyme (EC 3.2.1.31) was confirmed by isoelectric focusing. A time course study revealed that expression of the GUS gene could be detected 1.5 h after DNA uptake. Peak activity was obtained in rapeseed after 27 h and in carrot and soybean after 66–72 h. Optimal experimental conditions for protoplast density, PEG concentration and the order of DNA-PEG addition were analysed. GUS activity in transfected protoplasts was determined quantitatively by a spectrofluorimetric assay and qualitatively by X-Gluc staining. A comparison between the spectrofluorimetric assay and the number of blue cells per millilitre was conducted." @default.
- W2004149462 created "2016-06-24" @default.
- W2004149462 creator A5012231325 @default.
- W2004149462 creator A5026481324 @default.
- W2004149462 date "1993-01-01" @default.
- W2004149462 modified "2023-09-23" @default.
- W2004149462 title "PEG mediated DNA uptake and transient GUS expression in carrot, rapeseed and soybean protoplasts" @default.
- W2004149462 cites W1517642551 @default.
- W2004149462 cites W1539503448 @default.
- W2004149462 cites W1966431760 @default.
- W2004149462 cites W1970928967 @default.
- W2004149462 cites W1971071771 @default.
- W2004149462 cites W1972097051 @default.
- W2004149462 cites W1984887980 @default.
- W2004149462 cites W1986587865 @default.
- W2004149462 cites W1994796962 @default.
- W2004149462 cites W1995401337 @default.
- W2004149462 cites W2002335431 @default.
- W2004149462 cites W2002507269 @default.
- W2004149462 cites W2017684406 @default.
- W2004149462 cites W2024513234 @default.
- W2004149462 cites W2026311140 @default.
- W2004149462 cites W2026356203 @default.
- W2004149462 cites W2040469547 @default.
- W2004149462 cites W2047175573 @default.
- W2004149462 cites W2077545872 @default.
- W2004149462 cites W2085753352 @default.
- W2004149462 cites W2086976128 @default.
- W2004149462 cites W2093518891 @default.
- W2004149462 cites W2151797742 @default.
- W2004149462 cites W2162487912 @default.
- W2004149462 cites W2324095427 @default.
- W2004149462 cites W4293247451 @default.
- W2004149462 doi "https://doi.org/10.1016/0168-9452(93)90128-m" @default.
- W2004149462 hasPublicationYear "1993" @default.
- W2004149462 type Work @default.
- W2004149462 sameAs 2004149462 @default.
- W2004149462 citedByCount "32" @default.
- W2004149462 countsByYear W20041494622012 @default.
- W2004149462 countsByYear W20041494622014 @default.
- W2004149462 countsByYear W20041494622017 @default.
- W2004149462 countsByYear W20041494622019 @default.
- W2004149462 countsByYear W20041494622021 @default.
- W2004149462 crossrefType "journal-article" @default.
- W2004149462 hasAuthorship W2004149462A5012231325 @default.
- W2004149462 hasAuthorship W2004149462A5026481324 @default.
- W2004149462 hasConcept C104317684 @default.
- W2004149462 hasConcept C150194340 @default.
- W2004149462 hasConcept C153911025 @default.
- W2004149462 hasConcept C161733203 @default.
- W2004149462 hasConcept C173749725 @default.
- W2004149462 hasConcept C2778842764 @default.
- W2004149462 hasConcept C2779726014 @default.
- W2004149462 hasConcept C552990157 @default.
- W2004149462 hasConcept C55493867 @default.
- W2004149462 hasConcept C59822182 @default.
- W2004149462 hasConcept C86803240 @default.
- W2004149462 hasConceptScore W2004149462C104317684 @default.
- W2004149462 hasConceptScore W2004149462C150194340 @default.
- W2004149462 hasConceptScore W2004149462C153911025 @default.
- W2004149462 hasConceptScore W2004149462C161733203 @default.
- W2004149462 hasConceptScore W2004149462C173749725 @default.
- W2004149462 hasConceptScore W2004149462C2778842764 @default.
- W2004149462 hasConceptScore W2004149462C2779726014 @default.
- W2004149462 hasConceptScore W2004149462C552990157 @default.
- W2004149462 hasConceptScore W2004149462C55493867 @default.
- W2004149462 hasConceptScore W2004149462C59822182 @default.
- W2004149462 hasConceptScore W2004149462C86803240 @default.
- W2004149462 hasIssue "2" @default.
- W2004149462 hasLocation W20041494621 @default.
- W2004149462 hasOpenAccess W2004149462 @default.
- W2004149462 hasPrimaryLocation W20041494621 @default.
- W2004149462 hasRelatedWork W117508041 @default.
- W2004149462 hasRelatedWork W140519890 @default.
- W2004149462 hasRelatedWork W2013901030 @default.
- W2004149462 hasRelatedWork W2024709524 @default.
- W2004149462 hasRelatedWork W2045250526 @default.
- W2004149462 hasRelatedWork W2064024790 @default.
- W2004149462 hasRelatedWork W2104698745 @default.
- W2004149462 hasRelatedWork W2364959773 @default.
- W2004149462 hasRelatedWork W3146255080 @default.
- W2004149462 hasRelatedWork W4233981156 @default.
- W2004149462 hasVolume "89" @default.
- W2004149462 isParatext "false" @default.
- W2004149462 isRetracted "false" @default.
- W2004149462 magId "2004149462" @default.
- W2004149462 workType "article" @default.