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- W2004150897 abstract "Optical nanoscopy allows to study biological and functional processes of sub-cellular organelles. In structuredillumination microscopy (SIM) the sample is illuminated with a grid-like interference pattern to encode higher spatialfrequency information into observable Moire patterns. By acquiring multiple images and a computation trick a superresolvedimage is obtained. SIM provides resolution enhancement of 2X in each axis as compared to conventionalmicroscopes. For a visible light, SIM provides an optical resolution of 100 nm. The challenges associated with opticalnanoscopy of a living cell are photo-toxicity, special dye requirements and artifacts due to cell movement. SIM workswith conventional dyes and is a wide-field technique making it suitable for imaging living cells. In this work, we willdiscuss the opportunities and challenges of imaging living cells using SIM. Two applications of optical nanoscopy ofliving cells will be discussed; a) imaging of mitochondria in a keratinocyte cell andOptical microscopy based on fluorescence has emerged as a vital tool in modern bio-medical imaging and diagnosis.Super-resolution bio-imaging allows gathering information from sub-cellular organelles. In structured illuminationmicroscopy (SIM) the sample is illuminated with a grid-like interference patterns to encode higher spatial frequenciesinformation into observable images (Moire fringes). A super-resolved image is then decoded using computational trick.In this work, we used SIM to acquired super-resolved optical images of mitochondria from a live keratinocyte cell (seeFig 1). SIM provides resolution enhancement of 2X in each axis and contrast enhancement of 8X on a projected image.Time-lapsed imaging was used to study the dynamics of mitochondria in a live cell." @default.
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- W2004150897 date "2014-08-28" @default.
- W2004150897 modified "2023-09-23" @default.
- W2004150897 title "Optical nanoscopy of a living cell" @default.
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- W2004150897 doi "https://doi.org/10.1117/12.2062581" @default.
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