FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004161851> ?p ?o ?g. }

• W2004161851 endingPage "6182" @default.
• W2004161851 startingPage "6175" @default.
• W2004161851 abstract "Crystal structures of E13T (Glu13 was replaced by Thr13) and E13Q (Glu13 was replaced by Gln13) FMN binding proteins (FMN-bp) from Desulfovibrio vulgaris, strain Miyazaki F, were determined by the X-ray diffraction method. Geometrical factors related to photoinduced electron transfer from Trp32, Tyr35, and Trp106 to the excited isoalloxazine (Iso*) were compared among the three forms of FMN-bp. The rate of ET is considered to be fastest from Trp32 to Iso* in FMN-bp and then from Tyr35 and Trp106. The distances between Iso and Trp32 did not change appreciably (0.705-0.712 nm) among WT, E13T, and E13Q FMN-bps, though the distances between Iso and Tyr35 or Trp106 became a little shorter by ca. 0.01 nm in both mutated FMN-bps. The distances between the residue at 13 and the ET donors or acceptor in the mutated proteins, however, changed markedly, compared to WT. Hydrogen bonding pairs and distances between Iso and surrounding amino acids were not modified when Glu13 was replaced by Thr13 or Gln13. Effects of elimination of ionic charge at Glu13 on the ultrafast fluorescence dynamics in E13T and E13Q were investigated comparing to WT, by means of a fluorescence up-conversion method. Fluorescence lifetimes were tau(1) = 107 fs (alpha(1) = 0.86), tau(2) = 475 fs (alpha(2) = 0.12), and tau(3) = 30 ps (alpha(3) = 0.02) in E13T and tau(1) = 134 fs (alpha(1) = 0.85), alpha(2) = 746 fs (alpha(2) = 0.12), and tau(3) = 30 ps (alpha(3) = 0.03) in E13Q, which are compared to the reported lifetimes in WT, tau(1) = 168 fs (alpha(1) = 0.95) and alpha(2) = 1.4 ps (alpha(2) = 0.05). Average lifetimes (tau(AV) = Sigma(i=1)(2or3)alpha(i)tau(i)) were 0.75 ps in E13T, 1.10 ps in E13Q, and 0.23 ps in WT, which implies that tau(AV) was 3.3 times longer in E13T and 4.8 times longer in E13Q, compared to WT. The ultrafast fluorescence dynamics of WT did not change when solvent changed from H(2)O to D(2)O. Static ET rates (inverse of average lifetimes) were analyzed with static structures of the three systems of FMN-bp. Net electrostatic (ES) energies of Iso and Trp32, on which ET rates depend, were 0.0263 eV in WT, 0.322 eV in E13T, and 0.412 eV in E13Q. The calculated ET rates were in excellent agreement with the observed ones in all systems." @default.
• W2004161851 created "2016-06-24" @default.
• W2004161851 creator A5006241528 @default.
• W2004161851 creator A5011891382 @default.
• W2004161851 creator A5031049857 @default.
• W2004161851 creator A5034691817 @default.
• W2004161851 creator A5056200948 @default.
• W2004161851 creator A5069100768 @default.
• W2004161851 creator A5091017979 @default.
• W2004161851 creator A5091847080 @default.
• W2004161851 date "2010-04-16" @default.
• W2004161851 modified "2023-10-18" @default.
• W2004161851 title "Effects of the Disappearance of One Charge on Ultrafast Fluorescence Dynamics of the FMN Binding Protein" @default.
• W2004161851 cites W1528681221 @default.
• W2004161851 cites W1539796472 @default.
• W2004161851 cites W1963966153 @default.
• W2004161851 cites W1965543871 @default.
• W2004161851 cites W1967681306 @default.
• W2004161851 cites W1977075727 @default.
• W2004161851 cites W1992021800 @default.
• W2004161851 cites W1997631021 @default.
• W2004161851 cites W2002373243 @default.
• W2004161851 cites W2007284911 @default.
• W2004161851 cites W2011668639 @default.
• W2004161851 cites W2014123020 @default.
• W2004161851 cites W2015492400 @default.
• W2004161851 cites W2017452352 @default.
• W2004161851 cites W2019294381 @default.
• W2004161851 cites W2030183437 @default.
• W2004161851 cites W2031904472 @default.
• W2004161851 cites W2037569525 @default.
• W2004161851 cites W2042484071 @default.
• W2004161851 cites W2043673715 @default.
• W2004161851 cites W2044241959 @default.
• W2004161851 cites W2047854200 @default.
• W2004161851 cites W2053672057 @default.
• W2004161851 cites W2056302809 @default.
• W2004161851 cites W2058476130 @default.
• W2004161851 cites W2062851494 @default.
• W2004161851 cites W2069464272 @default.
• W2004161851 cites W2081143109 @default.
• W2004161851 cites W2090505184 @default.
• W2004161851 cites W2095216354 @default.
• W2004161851 cites W2124540342 @default.
• W2004161851 cites W2126549420 @default.
• W2004161851 cites W2127737840 @default.
• W2004161851 cites W2411302981 @default.
• W2004161851 doi "https://doi.org/10.1021/jp912137s" @default.
• W2004161851 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20397678" @default.
• W2004161851 hasPublicationYear "2010" @default.
• W2004161851 type Work @default.
• W2004161851 sameAs 2004161851 @default.
• W2004161851 citedByCount "40" @default.
• W2004161851 countsByYear W20041618512012 @default.
• W2004161851 countsByYear W20041618512013 @default.
• W2004161851 countsByYear W20041618512014 @default.
• W2004161851 countsByYear W20041618512015 @default.
• W2004161851 countsByYear W20041618512016 @default.
• W2004161851 countsByYear W20041618512017 @default.
• W2004161851 countsByYear W20041618512018 @default.
• W2004161851 countsByYear W20041618512019 @default.
• W2004161851 countsByYear W20041618512020 @default.
• W2004161851 countsByYear W20041618512021 @default.
• W2004161851 countsByYear W20041618512022 @default.
• W2004161851 crossrefType "journal-article" @default.
• W2004161851 hasAuthorship W2004161851A5006241528 @default.
• W2004161851 hasAuthorship W2004161851A5011891382 @default.
• W2004161851 hasAuthorship W2004161851A5031049857 @default.
• W2004161851 hasAuthorship W2004161851A5034691817 @default.
• W2004161851 hasAuthorship W2004161851A5056200948 @default.
• W2004161851 hasAuthorship W2004161851A5069100768 @default.
• W2004161851 hasAuthorship W2004161851A5091017979 @default.
• W2004161851 hasAuthorship W2004161851A5091847080 @default.
• W2004161851 hasConcept C112887158 @default.
• W2004161851 hasConcept C121332964 @default.
• W2004161851 hasConcept C123669783 @default.
• W2004161851 hasConcept C178790620 @default.
• W2004161851 hasConcept C181500209 @default.
• W2004161851 hasConcept C185544564 @default.
• W2004161851 hasConcept C185592680 @default.
• W2004161851 hasConcept C26873012 @default.
• W2004161851 hasConcept C2779892579 @default.
• W2004161851 hasConcept C2781018080 @default.
• W2004161851 hasConcept C2781243026 @default.
• W2004161851 hasConcept C32909587 @default.
• W2004161851 hasConcept C523546767 @default.
• W2004161851 hasConcept C54355233 @default.
• W2004161851 hasConcept C62520636 @default.
• W2004161851 hasConcept C71240020 @default.
• W2004161851 hasConcept C75473681 @default.
• W2004161851 hasConcept C8010536 @default.
• W2004161851 hasConcept C86803240 @default.
• W2004161851 hasConcept C91881484 @default.
• W2004161851 hasConceptScore W2004161851C112887158 @default.
• W2004161851 hasConceptScore W2004161851C121332964 @default.
• W2004161851 hasConceptScore W2004161851C123669783 @default.
• W2004161851 hasConceptScore W2004161851C178790620 @default.
• W2004161851 hasConceptScore W2004161851C181500209 @default.

• next