FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004171024> ?p ?o ?g. }

• W2004171024 endingPage "2012" @default.
• W2004171024 startingPage "2005" @default.
• W2004171024 abstract "Carbonyl reduction was investigated in the continuous cell lines V79, NCI-H322 and C2REV7 by using the ketone compound metyrapone as a substrate. Metyrapone reducing enzymes were characterized by evaluating the cosubstrate requirement and by testing the sensitivity of this reaction to specific inhibitors. All cell lines were found to produce metyrapol at a linear rate over a time course of at least 48 hr, when tested in cultured monolayers. In general, cytosolic metyrapone reduction exceeds microsomal activity several-fold in all three cell lines. Quercitrin turned out to be the strongest inhibitor in all fractions, except in NCI-H322 microsomes where it had no effect. Consequently, carbonyl reductase is suspected to be responsible for metyrapone reduction in the cytosol and microsomes of V79 and C2REV7 cells as well as in the cytosol of NCI-H322 cells. Simultaneous sensitivity towards quercitrin, dicoumarol, indomethacin and 5α-dihydrotestosterone in some cases points to the existence of different isozymes of carbonyl reductase. In NCI-H322 microsomes only dicoumarol and indomethacin decrease metyrapol formation, thus pointing to an isozyme of NAD (P)H:quinone-oxidoreductase. Concerning cosubstrate requirements metyrapone reducing enzymes show a strong preference for NADPH, thus confirming the involvement of carbonyl reductase in this reaction. In conclusion, carbonyl reduction of metyrapone in continuous cell lines is mediated by carbonyl reductases due to the common sensitivity towards the diagnostic inhibitor quercitrin and due to the strong preference for NADPH as cosubstrate. According to its maintenance in permanent cell lines carbonyl reductase seems to be an essential and constitutive enzyme, which probably fills an important role in normal cell physiology." @default.
• W2004171024 created "2016-06-24" @default.
• W2004171024 creator A5043827878 @default.
• W2004171024 creator A5063250889 @default.
• W2004171024 date "1992-11-01" @default.
• W2004171024 modified "2023-09-26" @default.
• W2004171024 title "Characterization of carbonyl reducing activity in continuous cell lines of human and rodent origin" @default.
• W2004171024 cites W1519712150 @default.
• W2004171024 cites W1557943810 @default.
• W2004171024 cites W1560594993 @default.
• W2004171024 cites W1561395636 @default.
• W2004171024 cites W1735278138 @default.
• W2004171024 cites W1775749144 @default.
• W2004171024 cites W1889970588 @default.
• W2004171024 cites W1895626021 @default.
• W2004171024 cites W1952901628 @default.
• W2004171024 cites W1965791965 @default.
• W2004171024 cites W1967660304 @default.
• W2004171024 cites W1976187418 @default.
• W2004171024 cites W1980831647 @default.
• W2004171024 cites W1988654354 @default.
• W2004171024 cites W1996334061 @default.
• W2004171024 cites W2008242719 @default.
• W2004171024 cites W2026191996 @default.
• W2004171024 cites W2026441415 @default.
• W2004171024 cites W2026711299 @default.
• W2004171024 cites W2038233779 @default.
• W2004171024 cites W2053536319 @default.
• W2004171024 cites W2062707211 @default.
• W2004171024 cites W2063427270 @default.
• W2004171024 cites W2066773406 @default.
• W2004171024 cites W2067137978 @default.
• W2004171024 cites W2095003531 @default.
• W2004171024 cites W2113257022 @default.
• W2004171024 cites W2121709251 @default.
• W2004171024 cites W2131319712 @default.
• W2004171024 cites W2135931532 @default.
• W2004171024 cites W2247536470 @default.
• W2004171024 cites W2261741305 @default.
• W2004171024 cites W80362966 @default.
• W2004171024 cites W87789975 @default.
• W2004171024 doi "https://doi.org/10.1016/0006-2952(92)90103-p" @default.
• W2004171024 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1449519" @default.
• W2004171024 hasPublicationYear "1992" @default.
• W2004171024 type Work @default.
• W2004171024 sameAs 2004171024 @default.
• W2004171024 citedByCount "5" @default.
• W2004171024 countsByYear W20041710242014 @default.
• W2004171024 countsByYear W20041710242018 @default.
• W2004171024 crossrefType "journal-article" @default.
• W2004171024 hasAuthorship W2004171024A5043827878 @default.
• W2004171024 hasAuthorship W2004171024A5063250889 @default.
• W2004171024 hasConcept C119795356 @default.
• W2004171024 hasConcept C134018914 @default.
• W2004171024 hasConcept C134651460 @default.
• W2004171024 hasConcept C181199279 @default.
• W2004171024 hasConcept C185592680 @default.
• W2004171024 hasConcept C197957613 @default.
• W2004171024 hasConcept C2776719974 @default.
• W2004171024 hasConcept C2777263532 @default.
• W2004171024 hasConcept C2777990912 @default.
• W2004171024 hasConcept C2778004101 @default.
• W2004171024 hasConcept C2781263971 @default.
• W2004171024 hasConcept C55493867 @default.
• W2004171024 hasConcept C71240020 @default.
• W2004171024 hasConcept C75520062 @default.
• W2004171024 hasConcept C86803240 @default.
• W2004171024 hasConcept C87644729 @default.
• W2004171024 hasConcept C98539663 @default.
• W2004171024 hasConceptScore W2004171024C119795356 @default.
• W2004171024 hasConceptScore W2004171024C134018914 @default.
• W2004171024 hasConceptScore W2004171024C134651460 @default.
• W2004171024 hasConceptScore W2004171024C181199279 @default.
• W2004171024 hasConceptScore W2004171024C185592680 @default.
• W2004171024 hasConceptScore W2004171024C197957613 @default.
• W2004171024 hasConceptScore W2004171024C2776719974 @default.
• W2004171024 hasConceptScore W2004171024C2777263532 @default.
• W2004171024 hasConceptScore W2004171024C2777990912 @default.
• W2004171024 hasConceptScore W2004171024C2778004101 @default.
• W2004171024 hasConceptScore W2004171024C2781263971 @default.
• W2004171024 hasConceptScore W2004171024C55493867 @default.
• W2004171024 hasConceptScore W2004171024C71240020 @default.
• W2004171024 hasConceptScore W2004171024C75520062 @default.
• W2004171024 hasConceptScore W2004171024C86803240 @default.
• W2004171024 hasConceptScore W2004171024C87644729 @default.
• W2004171024 hasConceptScore W2004171024C98539663 @default.
• W2004171024 hasIssue "10" @default.
• W2004171024 hasLocation W20041710241 @default.
• W2004171024 hasLocation W20041710242 @default.
• W2004171024 hasOpenAccess W2004171024 @default.
• W2004171024 hasPrimaryLocation W20041710241 @default.
• W2004171024 hasRelatedWork W1953994264 @default.
• W2004171024 hasRelatedWork W1978299857 @default.
• W2004171024 hasRelatedWork W1981947202 @default.
• W2004171024 hasRelatedWork W2004171024 @default.
• W2004171024 hasRelatedWork W2004650377 @default.
• W2004171024 hasRelatedWork W2013448271 @default.
• W2004171024 hasRelatedWork W2046306533 @default.

• next