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• W2004171302 abstract "Background and objectives Timing of peripheral blood stem cell (PBSC) harvest is typically based on quantification of peripheral blood (PB) CD34+ cells. CD34 enumeration is expensive, requires expertise and takes a minimum of 1–2 h to perform. The Sysmex XE2100 is an automated haematology analyser that can rapidly and inexpensively identify haematopoietic progenitor cell (HPC) populations in PB. The aim of this study was to examine if HPC can be used to optimize timing of PBSC harvest. Materials and methods White blood cell (WBC), HPC and CD34 counts were determined in a total of 60 mobilized donors. Data were analysed to examine the utility of WBC and HPC counts in predicting preharvest CD34+ counts. Results In adults presenting for autologous collection, a PB HPC threshold of > 30/µl predicts a preharvest CD34+ count of > 20/µl with sensitivity of 86% and positive predictive value (PPV) of 100%. Among paediatric patients with a diagnosis of neuroblastoma, an HPC threshold of > 16/µl yielded sensitivity and PPV of 100%, while in children with other diagnoses, an HPC cut-off of > 44/µl yielded sensitivity and PPV of 67% and 100%, respectively. Eighty per cent of adequately mobilized allogeneic donors were identified using an HPC threshold > 15/µl, with a PPV of 100%. PB WBC can also aid in predicting CD34 counts in most patient groups, albeit with lower sensitivity than HPC. Conclusion By virtue of being a sensitive and accurate predictor of preharvest CD34+ counts, our data support the use of the HPC parameter in optimizing the timing of PBSC harvest." @default.
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• W2004171302 date "2009-08-01" @default.
• W2004171302 modified "2023-10-15" @default.
• W2004171302 title "Use of the haematopoietic progenitor cell parameter in optimizing timing of peripheral blood stem cell harvest" @default.
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• W2004171302 doi "https://doi.org/10.1111/j.1423-0410.2009.01183.x" @default.
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