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• W2004173841 abstract "Abstract Plaque-forming λc I − mutants which carry internal deletions in the immunity region, including genes c I and rex , were isolated. Among seven independent isolates, only three demonstrably different deletions were obtained. The largest deletion (KH54 or KH115), which eliminates almost all of the DNA between promoters p L and p R , spans the distance from 74.1 to 78.4 %λ, as measured in relation to the left (0 %λ) and right (100 %λ) termini of the mature λ DNA molecule. There were four representatives of a second (74.7–77.7%λ) and one of a third (75.2–78.1%λ) deletion. In addition, a mutant carrying a 1400-nucleotide pair-long insertion in gene c I was isolated. The c I- rex deletions served as markers for more accurate measurements of the physical positions of the endpoints of several bio insertions, a dy plasmid, and the endonucleolytic cleavage sites for the restriction nucleases by electron micrography of DNA heteroduplexes. This led to a reevaluation of our earlier estimate of the interval length between the p L promoter mutation sex 1 and the s L startpoint for the major leftward operon of λ. In agreement with the independent results of others, we find that the p L to s L distance is within the limits of 24–62 nucleotide pairs ( s L - sex 1) or 11 to 45 nucleotide pairs ( s L - Hin II cut in o L )." @default.
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• W2004173841 date "1974-12-01" @default.
• W2004173841 modified "2023-09-24" @default.
• W2004173841 title "Deletions and insertions in the immunity region of coliphage lambda: Revised measurement of the promoter-startpoint distance" @default.
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• W2004173841 doi "https://doi.org/10.1016/0042-6822(74)90407-3" @default.
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