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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004177723> ?p ?o ?g. }

• W2004177723 endingPage "824" @default.
• W2004177723 startingPage "820" @default.
• W2004177723 abstract "In order to obtain indicator cell lines that are exquisitely susceptible to human T-lymphotropic virus type 1 (HTLV-1), luciferase gene driven by HTLV-1 long terminal repeat (LTR) was transfected into lymphocytic H9 cells with neo gene, and cell lines were selected by G418. A cell line (H9/K30luc) was found to produce an extremely high level of luciferase only when co-cultured with HTLV-1 producer MT-2 cells. Both in the absence and presence of a reverse transcriptase (RT) inhibitor azidothymidine, H9/K30luc cells generated similarly high luciferase activity upon co-cultivation with MT-2 cells. To develop an equivalent system for human immunodeficiency virus type 1 (HIV-1), H9/NL432 cells, which are stably infected with HIV-1 and producing a low level of the virus-like MT-2 cells for HTLV-1, were generated. Together with the indicator cell line H9/H1luc for HIV-1 already reported, antiviral effects of some agents on HTLV-1 and HIV-1 could be readily and sensitively evaluated by similar methods. In fact, by using our system, an HIV-1 protease inhibitor, saquinavir, was demonstrated to be highly effective against HIV-1 but not against HTLV-1." @default.
• W2004177723 created "2016-06-24" @default.
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• W2004177723 date "2005-05-01" @default.
• W2004177723 modified "2023-10-16" @default.
• W2004177723 title "Establishment of a biological assay system for human retroviral protease activity" @default.
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• W2004177723 doi "https://doi.org/10.1016/j.micinf.2005.02.008" @default.
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