Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004178930> ?p ?o ?g. }
- W2004178930 endingPage "62" @default.
- W2004178930 startingPage "52" @default.
- W2004178930 abstract "A successful parenteral formulation can be developed by studying stability and compatibility of biopharmaceuticals as a function of solution composition. Here, we evaluate the influence of pH, buffers, ionic strength, protein concentration and presence of excipients on recombinant human epidermal growth factor (rhEGF) stability. The stability was accessed by reversed-phase high performance liquid chromatography (RP-HPLC), size exclusion chromatography (SEC-HPLC), enzyme-linked immunosorbent assay (ELISA), Far-UV circular dichroism (CD) and light scattering. The overall maximal stability was obtained in pH near to 7.0 in phosphate, Tris and histidine buffers as the results of the different methods revealed. The CD results revealed that this protein is stable in an extensive pH range. Aggregation of rhEGF was minimized at pH values ranged from 6.0 to 8.0 as indicated the SEC-HPLC and light scattering results. Nor the ionic strength neither the rhEGF concentration had significant effect on the reaction rate constants. Most rhEGF-excipient instability occurs among this protein and reducing sugars. Polymers like poly(ethylene glycol) (PEG) and polysorbates increased methionine oxidation. The rhEGF oxidation and deamidation were the most common degradation pathways. This research identified critical solution factors to be considered for the development of a successful rhEGF parenteral formulation." @default.
- W2004178930 created "2016-06-24" @default.
- W2004178930 creator A5005379992 @default.
- W2004178930 creator A5009608666 @default.
- W2004178930 creator A5018608963 @default.
- W2004178930 creator A5024184169 @default.
- W2004178930 creator A5027767731 @default.
- W2004178930 creator A5047602351 @default.
- W2004178930 creator A5053093351 @default.
- W2004178930 creator A5065721595 @default.
- W2004178930 date "2013-08-01" @default.
- W2004178930 modified "2023-09-27" @default.
- W2004178930 title "Screening for stability and compatibility conditions of recombinant human epidermal growth factor for parenteral formulation: Effect of pH, buffers, and excipients" @default.
- W2004178930 cites W149157912 @default.
- W2004178930 cites W1501383613 @default.
- W2004178930 cites W1543535234 @default.
- W2004178930 cites W1553994079 @default.
- W2004178930 cites W1894641937 @default.
- W2004178930 cites W1965302158 @default.
- W2004178930 cites W1969050463 @default.
- W2004178930 cites W1970898345 @default.
- W2004178930 cites W1975085160 @default.
- W2004178930 cites W1976236482 @default.
- W2004178930 cites W1978269358 @default.
- W2004178930 cites W1980650956 @default.
- W2004178930 cites W1985941856 @default.
- W2004178930 cites W1986580592 @default.
- W2004178930 cites W1986768286 @default.
- W2004178930 cites W1987233346 @default.
- W2004178930 cites W2003917003 @default.
- W2004178930 cites W2016612023 @default.
- W2004178930 cites W2020390006 @default.
- W2004178930 cites W2023409474 @default.
- W2004178930 cites W2051756478 @default.
- W2004178930 cites W2052038646 @default.
- W2004178930 cites W2053669884 @default.
- W2004178930 cites W2065645372 @default.
- W2004178930 cites W2073092341 @default.
- W2004178930 cites W2081640562 @default.
- W2004178930 cites W2082503933 @default.
- W2004178930 cites W2083781709 @default.
- W2004178930 cites W2087591217 @default.
- W2004178930 cites W2089828431 @default.
- W2004178930 cites W2094077114 @default.
- W2004178930 cites W2106907072 @default.
- W2004178930 cites W2115786482 @default.
- W2004178930 cites W2127519720 @default.
- W2004178930 cites W2131262558 @default.
- W2004178930 cites W2138216710 @default.
- W2004178930 cites W2140712872 @default.
- W2004178930 cites W2167718987 @default.
- W2004178930 cites W4323254829 @default.
- W2004178930 doi "https://doi.org/10.1016/j.ijpharm.2013.04.054" @default.
- W2004178930 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23624083" @default.
- W2004178930 hasPublicationYear "2013" @default.
- W2004178930 type Work @default.
- W2004178930 sameAs 2004178930 @default.
- W2004178930 citedByCount "39" @default.
- W2004178930 countsByYear W20041789302014 @default.
- W2004178930 countsByYear W20041789302015 @default.
- W2004178930 countsByYear W20041789302016 @default.
- W2004178930 countsByYear W20041789302017 @default.
- W2004178930 countsByYear W20041789302018 @default.
- W2004178930 countsByYear W20041789302019 @default.
- W2004178930 countsByYear W20041789302020 @default.
- W2004178930 countsByYear W20041789302021 @default.
- W2004178930 countsByYear W20041789302022 @default.
- W2004178930 countsByYear W20041789302023 @default.
- W2004178930 crossrefType "journal-article" @default.
- W2004178930 hasAuthorship W2004178930A5005379992 @default.
- W2004178930 hasAuthorship W2004178930A5009608666 @default.
- W2004178930 hasAuthorship W2004178930A5018608963 @default.
- W2004178930 hasAuthorship W2004178930A5024184169 @default.
- W2004178930 hasAuthorship W2004178930A5027767731 @default.
- W2004178930 hasAuthorship W2004178930A5047602351 @default.
- W2004178930 hasAuthorship W2004178930A5053093351 @default.
- W2004178930 hasAuthorship W2004178930A5065721595 @default.
- W2004178930 hasConcept C127413603 @default.
- W2004178930 hasConcept C133571119 @default.
- W2004178930 hasConcept C14631669 @default.
- W2004178930 hasConcept C155672457 @default.
- W2004178930 hasConcept C178790620 @default.
- W2004178930 hasConcept C179998833 @default.
- W2004178930 hasConcept C181199279 @default.
- W2004178930 hasConcept C184651966 @default.
- W2004178930 hasConcept C185592680 @default.
- W2004178930 hasConcept C187428577 @default.
- W2004178930 hasConcept C193363816 @default.
- W2004178930 hasConcept C2777239854 @default.
- W2004178930 hasConcept C2777516009 @default.
- W2004178930 hasConcept C2780784506 @default.
- W2004178930 hasConcept C42360764 @default.
- W2004178930 hasConcept C43617362 @default.
- W2004178930 hasConcept C55493867 @default.
- W2004178930 hasConcept C77281830 @default.
- W2004178930 hasConceptScore W2004178930C127413603 @default.
- W2004178930 hasConceptScore W2004178930C133571119 @default.
- W2004178930 hasConceptScore W2004178930C14631669 @default.