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- W2004192483 abstract "We have used recombinant DNA techniques to construct a derivative of phage λ, called an excision vector, which retains only those functions necessary for conditional maintenance of lysogeny and integration/excision. The tyrA+ gene was cloned on this excision vector, integrated into the Escherichia coli chromosome, and stably maintained and expressed under permissive conditions. Upon shift to non-permissive conditions, the excision vector and its passenger gene were very efficiently excised from the chromosome and lost, leaving a culture of Tyr− bacteria. This illustrates a new class of conditional mutations in which the genotype changes in response to external stimuli." @default.
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- W2004192483 date "1988-07-01" @default.
- W2004192483 modified "2023-10-15" @default.
- W2004192483 title "Controllable alteration of cell genotype in bacterial cultures using an excision vector" @default.
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- W2004192483 doi "https://doi.org/10.1016/0378-1119(88)90012-1" @default.
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