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• W2004194037 abstract "Conversion of lignocellulosic material to ethanol is a major challenge in second generation bio-fuel production by yeast Saccharomyces cerevisiae. This report describes a novel strategy named “two-stage transcriptional reprogramming (TSTR)” in which key gene expression at both glucose and xylose fermentation phases is optimized in engineered S. cerevisiae. Through a combined genome-wide screening of stage-specific promoters and the balancing of the metabolic flux, ethanol yields and productivity from mixed sugars were significantly improved. In a medium containing 50 g/L glucose and 50 g/L xylose, the top-performing strain WXY12 rapidly consumed glucose within 12 h and within 84 h it consistently achieved an ethanol yield of 0.48 g/g total sugar, which was 94% of the theoretical yield. WXY12 utilizes a KGD1 inducible promoter to drive xylose metabolism, resulting in much higher ethanol yield than a reference strain using a strong constitutive PGK1 promoter. These promising results validate the TSTR strategy by synthetically regulating the xylose assimilation pathway towards efficient xylose fermentation." @default.
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• W2004194037 date "2014-07-01" @default.
• W2004194037 modified "2023-10-16" @default.
• W2004194037 title "Two-stage transcriptional reprogramming in Saccharomyces cerevisiae for optimizing ethanol production from xylose" @default.
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• W2004194037 doi "https://doi.org/10.1016/j.ymben.2014.05.001" @default.
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