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- W2004201211 abstract "Abstract Introduction The tandem mass spectrometer is a powerful tool with which to generate peptide (tandem) mass spectrum data for the analysis of complex biological protein mixtures in genomic-related disease cell lines. However, the majority of experimental tandem mass spectra cannot be interpreted by any database search engines. One of the main reasons this happens is that majority of experimental spectra are of quality too poor to be interpretable. Interpreting these “un-interpretable” spectra is a waste of time. Therefore, it is worthwhile to determine the quality of mass spectra before any interpretation. Objectives This paper proposes an approach to classifying tandem spectra into two groups: one with high quality and one with poor quality. Methods The proposed approach has two steps. First, each spectrum is mapped to a feature vector which describes the quality of the spectrum. Then, a weighted K-means clustering method is applied in order to classify the tandem mass spectra. Results and Conclusion Computational experiments illustrate that one cluster contains the majority of the high-quality spectra, while the other contains the majority of the poor-quality spectra. This result indicates that if we just search the spectra in the high-quality cluster, we can save the time for searching the majority of poor-quality spectra while losing a minimal amount of high-quality spectra. The software created for this work is available upon request." @default.
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- W2004201211 date "2009-03-01" @default.
- W2004201211 modified "2023-10-18" @default.
- W2004201211 title "Quality Assessment of Tandem Mass Spectra by Using a Weighted K-Means" @default.
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- W2004201211 doi "https://doi.org/10.1007/s12014-009-9025-4" @default.
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