Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004201567> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2004201567 endingPage "334" @default.
- W2004201567 startingPage "325" @default.
- W2004201567 abstract "A series of group-specific modifying reagents were tested for their effects on [3H]SCH23390 binding to brain D1 dopamine receptors in order to identify amino acid residues at the ligand binding site of the D1 dopamine receptor that are critical for ligand binding. The dependence of ligand binding on the pH of the incubation medium was also examined. The histidine-selective reagent, diethylpyrocarbonate did affect ligand binding but this is probably not due to an effect at the ligand binding site. Experiments with N-acetylimidazole and ethylacetimidate indicated that modification of tyrosine and amino residues did not exert major influences at the ligand binding site. The use of the thiol dithiothreitol indicated that breakage of a disulphide bond altered ligand binding, probably by affecting the receptor conformation, and the use of the sulphydryl reagent 5,5'-dithio-bis-nitrobenzoic acid showed that modification of a sulphydryl group on the receptor inhibited ligand binding. The carboxyl reagent N,N'-dicyclohexyl carbodiimide (DCCD) potently inhibited ligand binding and the effect could be prevented by occupancy of the receptor site by an agonist or antagonist so that there is an important carboxyl group at the receptor binding site. The total number of D1 receptors was reduced after the modification by DCCD and 70% of the residual receptors showed a reduced affinity for binding [3H]SCH23390, the remainder having the same affinity as untreated receptors. [3H]SCH23390 binding is also reduced by a decrease of pH and this effect seems to depend on the protonation of a group of pKa 6.9. Saturation analysis of [3H]SCH23390 binding performed at pH 7.5 shows a single class of high affinity sites whereas at pH 6.0, two classes of sites with higher and lower affinities are seen. These studies suggested a model whereby [3H]SCH23390 binding is to two receptor isoforms with different pH dependencies for [3H]SCH23390 binding." @default.
- W2004201567 created "2016-06-24" @default.
- W2004201567 creator A5007627492 @default.
- W2004201567 creator A5069448096 @default.
- W2004201567 date "1992-07-01" @default.
- W2004201567 modified "2023-09-27" @default.
- W2004201567 title "Studies on the structure of the ligand-binding site of the brain D1 dopamine receptor" @default.
- W2004201567 cites W1548179807 @default.
- W2004201567 cites W159198934 @default.
- W2004201567 cites W1604118064 @default.
- W2004201567 cites W160805338 @default.
- W2004201567 cites W1989094128 @default.
- W2004201567 cites W1993301425 @default.
- W2004201567 cites W2001566515 @default.
- W2004201567 cites W2017549779 @default.
- W2004201567 cites W2030905853 @default.
- W2004201567 cites W2040777203 @default.
- W2004201567 cites W2043813981 @default.
- W2004201567 cites W2045060141 @default.
- W2004201567 cites W2052411826 @default.
- W2004201567 cites W2055651755 @default.
- W2004201567 cites W2058688637 @default.
- W2004201567 cites W2062366275 @default.
- W2004201567 cites W2065219516 @default.
- W2004201567 cites W2065241932 @default.
- W2004201567 cites W2113630547 @default.
- W2004201567 cites W2317705625 @default.
- W2004201567 cites W4236519787 @default.
- W2004201567 doi "https://doi.org/10.1016/0006-2952(92)90016-c" @default.
- W2004201567 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1386511" @default.
- W2004201567 hasPublicationYear "1992" @default.
- W2004201567 type Work @default.
- W2004201567 sameAs 2004201567 @default.
- W2004201567 citedByCount "10" @default.
- W2004201567 crossrefType "journal-article" @default.
- W2004201567 hasAuthorship W2004201567A5007627492 @default.
- W2004201567 hasAuthorship W2004201567A5069448096 @default.
- W2004201567 hasConcept C107824862 @default.
- W2004201567 hasConcept C116569031 @default.
- W2004201567 hasConcept C120750228 @default.
- W2004201567 hasConcept C170493617 @default.
- W2004201567 hasConcept C181199279 @default.
- W2004201567 hasConcept C185592680 @default.
- W2004201567 hasConcept C2777824588 @default.
- W2004201567 hasConcept C2778938600 @default.
- W2004201567 hasConcept C55493867 @default.
- W2004201567 hasConcept C71240020 @default.
- W2004201567 hasConceptScore W2004201567C107824862 @default.
- W2004201567 hasConceptScore W2004201567C116569031 @default.
- W2004201567 hasConceptScore W2004201567C120750228 @default.
- W2004201567 hasConceptScore W2004201567C170493617 @default.
- W2004201567 hasConceptScore W2004201567C181199279 @default.
- W2004201567 hasConceptScore W2004201567C185592680 @default.
- W2004201567 hasConceptScore W2004201567C2777824588 @default.
- W2004201567 hasConceptScore W2004201567C2778938600 @default.
- W2004201567 hasConceptScore W2004201567C55493867 @default.
- W2004201567 hasConceptScore W2004201567C71240020 @default.
- W2004201567 hasIssue "2" @default.
- W2004201567 hasLocation W20042015671 @default.
- W2004201567 hasLocation W20042015672 @default.
- W2004201567 hasOpenAccess W2004201567 @default.
- W2004201567 hasPrimaryLocation W20042015671 @default.
- W2004201567 hasRelatedWork W1992681101 @default.
- W2004201567 hasRelatedWork W1994900313 @default.
- W2004201567 hasRelatedWork W1999653674 @default.
- W2004201567 hasRelatedWork W2004201567 @default.
- W2004201567 hasRelatedWork W2044083144 @default.
- W2004201567 hasRelatedWork W2048285970 @default.
- W2004201567 hasRelatedWork W2155039967 @default.
- W2004201567 hasRelatedWork W2411226154 @default.
- W2004201567 hasRelatedWork W2411621106 @default.
- W2004201567 hasRelatedWork W2417683782 @default.
- W2004201567 hasVolume "44" @default.
- W2004201567 isParatext "false" @default.
- W2004201567 isRetracted "false" @default.
- W2004201567 magId "2004201567" @default.
- W2004201567 workType "article" @default.