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- W2004207654 abstract "A sensitive and specific high‐performance liquid chromatographic procedure for the determination of chlorpropamide or tolbutamide in plasma in the presence of their metabolites is described. The ether extract of acidified plasma is redissolved in the mobile phase, 17% acetonitrile in 0.05 M aqueous ammonium formate, and chromatographed on a reverse‐phase column on a high‐performance liquid chromatograph fitted with a UV absorbance detector. Quantitation of plasma samples containing less than 0.5 μg/ml of chlorpropamide and 5 μg/ml of tolbutamide is reported, using these drugs as mutual internal standards. The retention times of the metabolites are such that they do not interfere in the procedure. The assay method was tested in a human volunteer with both drugs and found suitable for single‐dose pharmacokinetic studies." @default.
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- W2004207654 date "1976-09-01" @default.
- W2004207654 modified "2023-10-17" @default.
- W2004207654 title "Assay of Sulfonylureas in Human Plasma by High-Performance Liquid Chromatography" @default.
- W2004207654 doi "https://doi.org/10.1002/jps.2600650924" @default.
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