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• W2004222489 abstract "SUMMARY Aspects of vitellogenesis, known to be controlled by juvenile hormone, are adversely affected by Hymenolepis diminuta infection of Tenebrio molitor , in spite of circulating titres of the hormone remaining unchanged. It has therefore been proposed that juvenile hormone binding is disrupted at the tissue site level. Juvenile hormone III binding sites were located in the nuclear, microsomal and post-microsomal supernatant fractions of the follicle cells of Tenebrio molitor . When JH-III binding was quantified for both control and Hymenolepis diminuta -infected beetles, binding in the nucleus and cytosol were found to be largely unaffected. However, microsomal binding was severely disrupted; on days 3 and 6 post-infection, binding was greatly diminished, on day 9 post-infection, binding was slightly reduced and, by day 15, binding was ‘restored’ to that of control insects. Using follicle cell microsomes at day 3 post-infection, previous Scatchard analysis revealed the presence of at least two JH-III binding sites. The first is of higher affinity, K d = 5·3 x 10 ·5 x 10 −11 mol/mg protein and the second of lower affinity K d = 7·7 x 10 −7 M, B max = 9·75 x 10 −11 mol/mg protein. A comparison with microsomal binding parameters of follicle cells from non-infected Tenebrio indicated that although the B max values were unchanged, the K d value of the higher affinity site was increased by approximately 5-fold. These data are indicative of a parasite-induced competitive binding inhibitor." @default.
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• W2004222489 date "1995-06-01" @default.
• W2004222489 modified "2023-09-27" @default.
• W2004222489 title "Hymenolepis diminuta-induced fecundity reduction may be caused by changes in hormone binding toTenebrio molitorovaries" @default.
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• W2004222489 doi "https://doi.org/10.1017/s0031182000065288" @default.
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