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• W2004223516 endingPage "678" @default.
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• W2004223516 abstract "Shigellosis is one of major foodborne pathogens in both developed and developing countries. Although antibiotic therapy is considered an effective treatment for shigellosis, the imprudent use of antibiotics has led to the increase of multiple-antibiotic-resistant Shigella species globally. In this study, we isolated a virulent Podoviridae bacteriophage (phage), pSb-1, that infects Shigella boydii. One-step growth analysis revealed that this phage has a short latent period (15 min) and a large burst size (152.63 PFU/cell), indicating that pSb-1 has good host infectivity and effective lytic activity. The double-stranded DNA genome of pSb-1 is composed of 71,629 bp with a G + C content of 42.74%. The genome encodes 103 putative ORFs, 9 putative promoters, 21 transcriptional terminators, and one tRNA region. Genome sequence analysis of pSb-1 and comparative analysis with the homologous phage EC1-UPM, N4-like phage revealed that there is a high degree of similarity (94%, nucleotide sequence identity) between pSb-1 and EC1-UPM in 73 of the 103 ORFs of pSb-1. The results of this investigation indicate that pSb-1 is a novel virulent N4-like phage infecting S. boydii and that this phage might have potential uses against shigellosis." @default.
• W2004223516 created "2016-06-24" @default.
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• W2004223516 creator A5081385708 @default.
• W2004223516 date "2014-10-01" @default.
• W2004223516 modified "2023-10-17" @default.
• W2004223516 title "Characterization and complete genome sequence of a novel N4-like bacteriophage, pSb-1 infecting Shigella boydii" @default.
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• W2004223516 doi "https://doi.org/10.1016/j.resmic.2014.09.006" @default.
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