FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004229825> ?p ?o ?g. }

• W2004229825 endingPage "e0120091" @default.
• W2004229825 startingPage "e0120091" @default.
• W2004229825 abstract "Cell-cell adhesion is paramount in providing and maintaining multicellular structure and signal transmission between cells. In the skin, disruption to desmosomal regulated intercellular connectivity may lead to disorders of keratinization and hyperproliferative disease including cancer. Recently we showed transgenic mice overexpressing desmoglein 2 (Dsg2) in the epidermis develop hyperplasia. Following microarray and gene network analysis, we demonstrate that Dsg2 caused a profound change in the transcriptome of keratinocytes in vivo and altered a number of genes important in epithelial dysplasia including: calcium-binding proteins (S100A8 and S100A9), members of the cyclin protein family, and the cysteine protease inhibitor cystatin A (CSTA). CSTA is deregulated in several skin cancers, including squamous cell carcinomas (SCC) and loss of function mutations lead to recessive skin fragility disorders. The microarray results were confirmed by qPCR, immunoblotting, and immunohistochemistry. CSTA was detected at high level throughout the newborn mouse epidermis but dramatically decreased with development and was detected predominantly in the differentiated layers. In human keratinocytes, knockdown of Dsg2 by siRNA or shRNA reduced CSTA expression. Furthermore, siRNA knockdown of CSTA resulted in cytoplasmic localization of Dsg2, perturbed cytokeratin 14 staining and reduced levels of desmoplakin in response to mechanical stretching. Both knockdown of either Dsg2 or CSTA induced loss of cell adhesion in a dispase-based assay and the effect was synergistic. Our findings here offer a novel pathway of CSTA regulation involving Dsg2 and a potential crosstalk between Dsg2 and CSTA that modulates cell adhesion. These results further support the recent human genetic findings that loss of function mutations in the CSTA gene result in skin fragility due to impaired cell-cell adhesion: autosomal-recessive exfoliative ichthyosis or acral peeling skin syndrome." @default.
• W2004229825 created "2016-06-24" @default.
• W2004229825 creator A5033238350 @default.
• W2004229825 creator A5037957293 @default.
• W2004229825 creator A5042446012 @default.
• W2004229825 creator A5056378781 @default.
• W2004229825 creator A5058022491 @default.
• W2004229825 creator A5064547820 @default.
• W2004229825 creator A5075435082 @default.
• W2004229825 date "2015-03-18" @default.
• W2004229825 modified "2023-10-16" @default.
• W2004229825 title "Cell Cycle- and Cancer-Associated Gene Networks Activated by Dsg2: Evidence of Cystatin A Deregulation and a Potential Role in Cell-Cell Adhesion" @default.
• W2004229825 cites W1555837120 @default.
• W2004229825 cites W1579139742 @default.
• W2004229825 cites W1684303833 @default.
• W2004229825 cites W1892030779 @default.
• W2004229825 cites W1963922571 @default.
• W2004229825 cites W1971386513 @default.
• W2004229825 cites W1972009876 @default.
• W2004229825 cites W1976453031 @default.
• W2004229825 cites W1976994179 @default.
• W2004229825 cites W1982493220 @default.
• W2004229825 cites W1983426701 @default.
• W2004229825 cites W1989066034 @default.
• W2004229825 cites W1989924429 @default.
• W2004229825 cites W1992165963 @default.
• W2004229825 cites W1993031861 @default.
• W2004229825 cites W1994866501 @default.
• W2004229825 cites W2002616945 @default.
• W2004229825 cites W2012278879 @default.
• W2004229825 cites W2017326894 @default.
• W2004229825 cites W2017451311 @default.
• W2004229825 cites W2020719547 @default.
• W2004229825 cites W2021722828 @default.
• W2004229825 cites W2024297705 @default.
• W2004229825 cites W2032833822 @default.
• W2004229825 cites W2033096907 @default.
• W2004229825 cites W2035099241 @default.
• W2004229825 cites W2036872340 @default.
• W2004229825 cites W2042737353 @default.
• W2004229825 cites W2043634223 @default.
• W2004229825 cites W2045622959 @default.
• W2004229825 cites W2046665391 @default.
• W2004229825 cites W2058616210 @default.
• W2004229825 cites W2060073547 @default.
• W2004229825 cites W2067365713 @default.
• W2004229825 cites W2070293972 @default.
• W2004229825 cites W2074718734 @default.
• W2004229825 cites W2075550764 @default.
• W2004229825 cites W2076636899 @default.
• W2004229825 cites W2077075786 @default.
• W2004229825 cites W2080412054 @default.
• W2004229825 cites W2084104855 @default.
• W2004229825 cites W2087716264 @default.
• W2004229825 cites W2093381141 @default.
• W2004229825 cites W2094514545 @default.
• W2004229825 cites W2097865892 @default.
• W2004229825 cites W2099156318 @default.
• W2004229825 cites W2115229273 @default.
• W2004229825 cites W2118030557 @default.
• W2004229825 cites W2135233980 @default.
• W2004229825 cites W2149957914 @default.
• W2004229825 cites W2150268846 @default.
• W2004229825 cites W2157508031 @default.
• W2004229825 cites W2159918368 @default.
• W2004229825 cites W2160728627 @default.
• W2004229825 cites W2162776161 @default.
• W2004229825 cites W2164663952 @default.
• W2004229825 cites W2319850978 @default.
• W2004229825 cites W2994158156 @default.
• W2004229825 cites W4232058283 @default.
• W2004229825 cites W4323529747 @default.
• W2004229825 doi "https://doi.org/10.1371/journal.pone.0120091" @default.
• W2004229825 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4364902" @default.
• W2004229825 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25785582" @default.
• W2004229825 hasPublicationYear "2015" @default.
• W2004229825 type Work @default.
• W2004229825 sameAs 2004229825 @default.
• W2004229825 citedByCount "20" @default.
• W2004229825 countsByYear W20042298252015 @default.
• W2004229825 countsByYear W20042298252016 @default.
• W2004229825 countsByYear W20042298252017 @default.
• W2004229825 countsByYear W20042298252018 @default.
• W2004229825 countsByYear W20042298252019 @default.
• W2004229825 countsByYear W20042298252020 @default.
• W2004229825 countsByYear W20042298252021 @default.
• W2004229825 countsByYear W20042298252022 @default.
• W2004229825 countsByYear W20042298252023 @default.
• W2004229825 crossrefType "journal-article" @default.
• W2004229825 hasAuthorship W2004229825A5033238350 @default.
• W2004229825 hasAuthorship W2004229825A5037957293 @default.
• W2004229825 hasAuthorship W2004229825A5042446012 @default.
• W2004229825 hasAuthorship W2004229825A5056378781 @default.
• W2004229825 hasAuthorship W2004229825A5058022491 @default.
• W2004229825 hasAuthorship W2004229825A5064547820 @default.
• W2004229825 hasAuthorship W2004229825A5075435082 @default.
• W2004229825 hasBestOaLocation W20042298251 @default.
• W2004229825 hasConcept C1491633281 @default.

• next