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• W2004231633 abstract "Summary The purpose of this study was to prepare intracellular pathogen resistance 1 ( Ipr1 ) transgenic donor cells for somatic cell nuclear transfer (SCNT). Based on our current understanding of Ipr1, a macrophage special expression vector pSP–EGFP–Ipr1was constructed. Bovine fetal fibroblasts were transfected with pSP-EGFP-Ipr1. The green fluorescent protein (GFP)-expressing cells were selected and transferred into enucleated bovine oocytes. Then, the rates of oocyte cleavage and blastocyst formation of transgenic cells and non-transgenic cells were observed, respectively. The results showed that reconstructed embryos derived from transgenic cells could successfully develop into blastocysts, most of which were GFP-positive. This study may provide cloned embryos for the production of anti-tuberculosis transgenic animals." @default.
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• W2004231633 date "2011-08-26" @default.
• W2004231633 modified "2023-09-29" @default.
• W2004231633 title "Construction of Ipr1 expression vector and development of cloned embryos in vitro" @default.
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• W2004231633 doi "https://doi.org/10.1017/s0967199411000529" @default.
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